Effect of CO2 concentration on phosphatidylcholine and phosphatidylglycerol metabolism in surfactant and residual lung fractions
An investigation of the effect of change of total CO2 concentration from 7 to 43 mM at pH 7.35 in the medium perfusing isolated rat lungs on [U-14C]glucose incorporation into lung phospholipids has been carried out. The incorporation of [U-14C]glucose into phosphatidylcholine and phosphatidylglycero...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
1977-05-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520416803 |
| _version_ | 1818596164241457152 |
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| author | W J Longmore J T Mourning |
| author_facet | W J Longmore J T Mourning |
| author_sort | W J Longmore |
| collection | DOAJ |
| description | An investigation of the effect of change of total CO2 concentration from 7 to 43 mM at pH 7.35 in the medium perfusing isolated rat lungs on [U-14C]glucose incorporation into lung phospholipids has been carried out. The incorporation of [U-14C]glucose into phosphatidylcholine and phosphatidylglycerol of the surfactant fraction and of the remaining lung tissue (residual fraction) was observed. Increased CO2 concentration increased [U-14C]glucose incorporation into phosphatidylcholine of the surfactant fraction and residual fraction by 43 and 50%, respectively, during a 2 hr perfusion. Likewise, incorporation of [U-14C]glucose into phosphatidylglycerol was increased 22 and 34% into the surfactant and residual fractions, respectively. The percentage of [U-14C]glucose incorporated into the fatty acid moieties of phosphatidylcholine of both fractions increased as a result of increased CO2 concentration. The increase in the incorporation of [U-14C]glucose into the fatty acid moieties of phosphatidylcholine was confirmed by an average increase of 56 and 77% in the specific activity of palmitic acid isolated from phosphatidylcholine of the surfactant and residual fraction, respectively, as a result of increased CO2 concentration. The results suggest that alteration in extracellular CO2 concentration affects the de novo synthesis from glucose of phosphatidylcholine and phosphatidylglycerol of the surfactant-lipoprotein fraction of lung. |
| first_indexed | 2024-12-16T11:27:33Z |
| format | Article |
| id | doaj.art-0dd13555a38d487393732fad3c98ec5b |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-16T11:27:33Z |
| publishDate | 1977-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-0dd13555a38d487393732fad3c98ec5b2022-12-21T22:33:19ZengElsevierJournal of Lipid Research0022-22751977-05-01183309313Effect of CO2 concentration on phosphatidylcholine and phosphatidylglycerol metabolism in surfactant and residual lung fractionsW J Longmore0J T Mourning1Department of Biochemistry, St. Louis University School of Medicine, St. Louis, MO 63104Department of Biochemistry, St. Louis University School of Medicine, St. Louis, MO 63104An investigation of the effect of change of total CO2 concentration from 7 to 43 mM at pH 7.35 in the medium perfusing isolated rat lungs on [U-14C]glucose incorporation into lung phospholipids has been carried out. The incorporation of [U-14C]glucose into phosphatidylcholine and phosphatidylglycerol of the surfactant fraction and of the remaining lung tissue (residual fraction) was observed. Increased CO2 concentration increased [U-14C]glucose incorporation into phosphatidylcholine of the surfactant fraction and residual fraction by 43 and 50%, respectively, during a 2 hr perfusion. Likewise, incorporation of [U-14C]glucose into phosphatidylglycerol was increased 22 and 34% into the surfactant and residual fractions, respectively. The percentage of [U-14C]glucose incorporated into the fatty acid moieties of phosphatidylcholine of both fractions increased as a result of increased CO2 concentration. The increase in the incorporation of [U-14C]glucose into the fatty acid moieties of phosphatidylcholine was confirmed by an average increase of 56 and 77% in the specific activity of palmitic acid isolated from phosphatidylcholine of the surfactant and residual fraction, respectively, as a result of increased CO2 concentration. The results suggest that alteration in extracellular CO2 concentration affects the de novo synthesis from glucose of phosphatidylcholine and phosphatidylglycerol of the surfactant-lipoprotein fraction of lung.http://www.sciencedirect.com/science/article/pii/S0022227520416803[U-14C]glucosepalmitic aciddiplamitoyl phosphatidylcholine |
| spellingShingle | W J Longmore J T Mourning Effect of CO2 concentration on phosphatidylcholine and phosphatidylglycerol metabolism in surfactant and residual lung fractions Journal of Lipid Research [U-14C]glucose palmitic acid diplamitoyl phosphatidylcholine |
| title | Effect of CO2 concentration on phosphatidylcholine and phosphatidylglycerol metabolism in surfactant and residual lung fractions |
| title_full | Effect of CO2 concentration on phosphatidylcholine and phosphatidylglycerol metabolism in surfactant and residual lung fractions |
| title_fullStr | Effect of CO2 concentration on phosphatidylcholine and phosphatidylglycerol metabolism in surfactant and residual lung fractions |
| title_full_unstemmed | Effect of CO2 concentration on phosphatidylcholine and phosphatidylglycerol metabolism in surfactant and residual lung fractions |
| title_short | Effect of CO2 concentration on phosphatidylcholine and phosphatidylglycerol metabolism in surfactant and residual lung fractions |
| title_sort | effect of co2 concentration on phosphatidylcholine and phosphatidylglycerol metabolism in surfactant and residual lung fractions |
| topic | [U-14C]glucose palmitic acid diplamitoyl phosphatidylcholine |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520416803 |
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