A key regulatory loop AK4P1/miR-375/SP1 in pancreatic adenocarcinoma
Pancreatic adenocarcinoma is one of the leading lethal human cancer types and is notorious for its poor prognosis. A series of bioinformatic analyses and experimental validations were employed to explore the role and mechanism of pseudogene-derived RNAs in pancreatic adenocarcinoma. Consequently, a...
| 主要な著者: | , , |
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| フォーマット: | 論文 |
| 言語: | English |
| 出版事項: |
Taylor & Francis Group
2023-12-01
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| シリーズ: | Epigenetics |
| 主題: | |
| オンライン･アクセス: | http://dx.doi.org/10.1080/15592294.2022.2148433 |
| _version_ | 1827811434448289792 |
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| author | Wangjin Xu Weiyang Lou Linhang Mei |
| author_facet | Wangjin Xu Weiyang Lou Linhang Mei |
| author_sort | Wangjin Xu |
| collection | DOAJ |
| description | Pancreatic adenocarcinoma is one of the leading lethal human cancer types and is notorious for its poor prognosis. A series of bioinformatic analyses and experimental validations were employed to explore the role and mechanism of pseudogene-derived RNAs in pancreatic adenocarcinoma. Consequently, a total of 13 upregulated and 7 downregulated pseudogene-derived RNAs in pancreatic adenocarcinoma were identified. Survival analysis revealed a statistically predictive role of AK4P1 for unfavourable prognosis of patients with pancreatic adenocarcinoma. Subcellular location analysis indicated that AK4P1 was mainly located in cytoplasm, in which AK4P1 might competitively bind to tumour suppressive miR-375 in pancreatic adenocarcinoma. Further analysis showed that SP1 was a potential downstream target gene of miR-375 in pancreatic adenocarcinoma. Intriguingly, expression determination validated that SP1 could positively regulate AK4P1 levels in pancreatic adenocarcinoma. Finally, AK4P1 might also exert its effects by interacting with oncogenic parental gene AK4 in pancreatic adenocarcinoma. Conclusively, the present study elucidated a key regulatory loop AK4P1/miR-375/SP1 in pancreatic adenocarcinoma. |
| first_indexed | 2024-03-11T23:05:10Z |
| format | Article |
| id | doaj.art-0dd52ad8739e462a8b45fc975ab901b2 |
| institution | Directory Open Access Journal |
| issn | 1559-2294 1559-2308 |
| language | English |
| last_indexed | 2024-03-11T23:05:10Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Epigenetics |
| spelling | doaj.art-0dd52ad8739e462a8b45fc975ab901b22023-09-21T13:23:12ZengTaylor & Francis GroupEpigenetics1559-22941559-23082023-12-0118110.1080/15592294.2022.21484332148433A key regulatory loop AK4P1/miR-375/SP1 in pancreatic adenocarcinomaWangjin Xu0Weiyang Lou1Linhang Mei2Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical UniversityThe First Affiliated Hospital, College of Medicine, Zhejiang UniversityTaizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical UniversityPancreatic adenocarcinoma is one of the leading lethal human cancer types and is notorious for its poor prognosis. A series of bioinformatic analyses and experimental validations were employed to explore the role and mechanism of pseudogene-derived RNAs in pancreatic adenocarcinoma. Consequently, a total of 13 upregulated and 7 downregulated pseudogene-derived RNAs in pancreatic adenocarcinoma were identified. Survival analysis revealed a statistically predictive role of AK4P1 for unfavourable prognosis of patients with pancreatic adenocarcinoma. Subcellular location analysis indicated that AK4P1 was mainly located in cytoplasm, in which AK4P1 might competitively bind to tumour suppressive miR-375 in pancreatic adenocarcinoma. Further analysis showed that SP1 was a potential downstream target gene of miR-375 in pancreatic adenocarcinoma. Intriguingly, expression determination validated that SP1 could positively regulate AK4P1 levels in pancreatic adenocarcinoma. Finally, AK4P1 might also exert its effects by interacting with oncogenic parental gene AK4 in pancreatic adenocarcinoma. Conclusively, the present study elucidated a key regulatory loop AK4P1/miR-375/SP1 in pancreatic adenocarcinoma.http://dx.doi.org/10.1080/15592294.2022.2148433ak4p1mir-375sp1pancreatic adenocarcinomaprognosis |
| spellingShingle | Wangjin Xu Weiyang Lou Linhang Mei A key regulatory loop AK4P1/miR-375/SP1 in pancreatic adenocarcinoma Epigenetics ak4p1 mir-375 sp1 pancreatic adenocarcinoma prognosis |
| title | A key regulatory loop AK4P1/miR-375/SP1 in pancreatic adenocarcinoma |
| title_full | A key regulatory loop AK4P1/miR-375/SP1 in pancreatic adenocarcinoma |
| title_fullStr | A key regulatory loop AK4P1/miR-375/SP1 in pancreatic adenocarcinoma |
| title_full_unstemmed | A key regulatory loop AK4P1/miR-375/SP1 in pancreatic adenocarcinoma |
| title_short | A key regulatory loop AK4P1/miR-375/SP1 in pancreatic adenocarcinoma |
| title_sort | key regulatory loop ak4p1 mir 375 sp1 in pancreatic adenocarcinoma |
| topic | ak4p1 mir-375 sp1 pancreatic adenocarcinoma prognosis |
| url | http://dx.doi.org/10.1080/15592294.2022.2148433 |
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