Comparison of acridine orange fluorescent microscopy and gram stain light microscopy for the rapid detection of bacteria in cerebrospinal fluid
Abstract Objective Bacterial meningitis is a life threatening condition that requires prompt recognition and treatment. Currently, Gram stain is widely used for the microscopic detection of bacterial pathogens in cerebrospinal fluid (CSF). In Nepal, fluorescent microscopes have been installed in lab...
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| Format: | Article |
| Language: | English |
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BMC
2020-01-01
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| Series: | BMC Research Notes |
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| Online Access: | https://doi.org/10.1186/s13104-020-4895-7 |
| _version_ | 1818383100253569024 |
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| author | Supriya Sharma Jyoti Acharya Megha Raj Banjara Prakash Ghimire Anjana Singh |
| author_facet | Supriya Sharma Jyoti Acharya Megha Raj Banjara Prakash Ghimire Anjana Singh |
| author_sort | Supriya Sharma |
| collection | DOAJ |
| description | Abstract Objective Bacterial meningitis is a life threatening condition that requires prompt recognition and treatment. Currently, Gram stain is widely used for the microscopic detection of bacterial pathogens in cerebrospinal fluid (CSF). In Nepal, fluorescent microscopes have been installed in laboratories as a part of the National tuberculosis control program. However, information on the utility of the acridine orange (AO) stain for the direct detection of bacteria in CSF samples in Nepal is not available. Therefore, this study aims to compare Gram stain and AO stain for the rapid detection of bacterial pathogens in CSF of clinically suspected meningitis cases in Kathmandu, Nepal. Results Bacterial pathogens were detected in 9.30% (36/387) by either of the three tests, 9.04% (35/387) by AO stain, 8.27% (32/387) by culture and 6.46% (25/387) by Gram’s stain. Considering culture as a gold standard, the sensitivity of AO stain was higher than Gram stain. The specificity of AO stain was 98.87%. Detection and differentiation of the bacteria was much clear in AO staining than Gram staining. AO is a better alternative to Gram stain in the rapid detection of bacterial pathogens in CSF in the setting where fluorescent microscope is available. |
| first_indexed | 2024-12-14T03:01:00Z |
| format | Article |
| id | doaj.art-0dd6b651d5904b5cb98d1088a8b2c69d |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-14T03:01:00Z |
| publishDate | 2020-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-0dd6b651d5904b5cb98d1088a8b2c69d2022-12-21T23:19:31ZengBMCBMC Research Notes1756-05002020-01-011311510.1186/s13104-020-4895-7Comparison of acridine orange fluorescent microscopy and gram stain light microscopy for the rapid detection of bacteria in cerebrospinal fluidSupriya Sharma0Jyoti Acharya1Megha Raj Banjara2Prakash Ghimire3Anjana Singh4Central Department of Microbiology, Tribhuvan UniversityNational Public Health LaboratoryCentral Department of Microbiology, Tribhuvan UniversityCentral Department of Microbiology, Tribhuvan UniversityCentral Department of Microbiology, Tribhuvan UniversityAbstract Objective Bacterial meningitis is a life threatening condition that requires prompt recognition and treatment. Currently, Gram stain is widely used for the microscopic detection of bacterial pathogens in cerebrospinal fluid (CSF). In Nepal, fluorescent microscopes have been installed in laboratories as a part of the National tuberculosis control program. However, information on the utility of the acridine orange (AO) stain for the direct detection of bacteria in CSF samples in Nepal is not available. Therefore, this study aims to compare Gram stain and AO stain for the rapid detection of bacterial pathogens in CSF of clinically suspected meningitis cases in Kathmandu, Nepal. Results Bacterial pathogens were detected in 9.30% (36/387) by either of the three tests, 9.04% (35/387) by AO stain, 8.27% (32/387) by culture and 6.46% (25/387) by Gram’s stain. Considering culture as a gold standard, the sensitivity of AO stain was higher than Gram stain. The specificity of AO stain was 98.87%. Detection and differentiation of the bacteria was much clear in AO staining than Gram staining. AO is a better alternative to Gram stain in the rapid detection of bacterial pathogens in CSF in the setting where fluorescent microscope is available.https://doi.org/10.1186/s13104-020-4895-7Bacterial meningitisCultureAcridine orange stainGram stain |
| spellingShingle | Supriya Sharma Jyoti Acharya Megha Raj Banjara Prakash Ghimire Anjana Singh Comparison of acridine orange fluorescent microscopy and gram stain light microscopy for the rapid detection of bacteria in cerebrospinal fluid BMC Research Notes Bacterial meningitis Culture Acridine orange stain Gram stain |
| title | Comparison of acridine orange fluorescent microscopy and gram stain light microscopy for the rapid detection of bacteria in cerebrospinal fluid |
| title_full | Comparison of acridine orange fluorescent microscopy and gram stain light microscopy for the rapid detection of bacteria in cerebrospinal fluid |
| title_fullStr | Comparison of acridine orange fluorescent microscopy and gram stain light microscopy for the rapid detection of bacteria in cerebrospinal fluid |
| title_full_unstemmed | Comparison of acridine orange fluorescent microscopy and gram stain light microscopy for the rapid detection of bacteria in cerebrospinal fluid |
| title_short | Comparison of acridine orange fluorescent microscopy and gram stain light microscopy for the rapid detection of bacteria in cerebrospinal fluid |
| title_sort | comparison of acridine orange fluorescent microscopy and gram stain light microscopy for the rapid detection of bacteria in cerebrospinal fluid |
| topic | Bacterial meningitis Culture Acridine orange stain Gram stain |
| url | https://doi.org/10.1186/s13104-020-4895-7 |
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