Bacterial Community Structure and Dynamic Changes in Different Functional Areas of a Piggery Wastewater Treatment System
Chemicals of emerging concern (CEC) in pig farm breeding wastewater, such as antibiotics, will soon pose a serious threat to public health. It is therefore essential to consider improving the treatment efficiency of piggery wastewater in terms of microorganisms. In order to optimize the overall pigg...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-10-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/9/10/2134 |
| _version_ | 1797513735340818432 |
|---|---|
| author | Lin Shi Naiyuan Liu Gang Liu Jun Fang |
| author_facet | Lin Shi Naiyuan Liu Gang Liu Jun Fang |
| author_sort | Lin Shi |
| collection | DOAJ |
| description | Chemicals of emerging concern (CEC) in pig farm breeding wastewater, such as antibiotics, will soon pose a serious threat to public health. It is therefore essential to consider improving the treatment efficiency of piggery wastewater in terms of microorganisms. In order to optimize the overall piggery wastewater treatment system from the perspective of the bacterial community structure and its response to environmental factors, five samples were randomly taken from each area of a piggery’s wastewater treatment system using a random sampling method. The bacterial communities’ composition and their correlation with wastewater quality were then analyzed using Illumina MiSeq high-throughput sequencing. The results showed that the bacterial community composition of each treatment unit was similar. However, differences in abundance were significant, and the bacterial community structure gradually changed with the process. <i>Proteobacteria</i> showed more adaptability to an anaerobic environment than <i>Firmicutes</i>, and the abundance of <i>Tissierella</i> in anaerobic zones was low. The abundance of <i>Clostridial</i> (39.02%) and <i>Bacteroides</i> (20.6%) in the inlet was significantly higher than it was in the aerobic zone and the anoxic zone (<i>p</i> < 0.05). <i>Rhodocyclaceae</i> is a key functional microbial group in a wastewater treatment system, and it is a dominant microbial group in activated sludge. Redundancy analysis (RDA) showed that chemical oxygen demand (COD) had the greatest impact on bacterial community structure. Total phosphorus (TP), total nitrogen (TN), PH and COD contents were significantly negatively correlated with <i>Sphingobacteriia</i>, <i>Betaproteobacteria</i> and <i>Gammaproteobacteria</i>, and significantly positively correlated with <i>Bacteroidia</i> and <i>Clostridia</i>. These results offer basic data and theoretical support for optimizing livestock wastewater treatment systems using bacterial community structures. |
| first_indexed | 2024-03-10T06:21:43Z |
| format | Article |
| id | doaj.art-0dd7265ad08f49b5b2be93ba4aa3eaca |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T06:21:43Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-0dd7265ad08f49b5b2be93ba4aa3eaca2023-11-22T19:14:52ZengMDPI AGMicroorganisms2076-26072021-10-01910213410.3390/microorganisms9102134Bacterial Community Structure and Dynamic Changes in Different Functional Areas of a Piggery Wastewater Treatment SystemLin Shi0Naiyuan Liu1Gang Liu2Jun Fang3Hunan Provincial Engineering Research Center of Applied Microbial Resources Development for Livestock and Poultry, College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410125, ChinaHunan Provincial Engineering Research Center of Applied Microbial Resources Development for Livestock and Poultry, College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410125, ChinaHunan Provincial Engineering Research Center of Applied Microbial Resources Development for Livestock and Poultry, College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410125, ChinaHunan Provincial Engineering Research Center of Applied Microbial Resources Development for Livestock and Poultry, College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410125, ChinaChemicals of emerging concern (CEC) in pig farm breeding wastewater, such as antibiotics, will soon pose a serious threat to public health. It is therefore essential to consider improving the treatment efficiency of piggery wastewater in terms of microorganisms. In order to optimize the overall piggery wastewater treatment system from the perspective of the bacterial community structure and its response to environmental factors, five samples were randomly taken from each area of a piggery’s wastewater treatment system using a random sampling method. The bacterial communities’ composition and their correlation with wastewater quality were then analyzed using Illumina MiSeq high-throughput sequencing. The results showed that the bacterial community composition of each treatment unit was similar. However, differences in abundance were significant, and the bacterial community structure gradually changed with the process. <i>Proteobacteria</i> showed more adaptability to an anaerobic environment than <i>Firmicutes</i>, and the abundance of <i>Tissierella</i> in anaerobic zones was low. The abundance of <i>Clostridial</i> (39.02%) and <i>Bacteroides</i> (20.6%) in the inlet was significantly higher than it was in the aerobic zone and the anoxic zone (<i>p</i> < 0.05). <i>Rhodocyclaceae</i> is a key functional microbial group in a wastewater treatment system, and it is a dominant microbial group in activated sludge. Redundancy analysis (RDA) showed that chemical oxygen demand (COD) had the greatest impact on bacterial community structure. Total phosphorus (TP), total nitrogen (TN), PH and COD contents were significantly negatively correlated with <i>Sphingobacteriia</i>, <i>Betaproteobacteria</i> and <i>Gammaproteobacteria</i>, and significantly positively correlated with <i>Bacteroidia</i> and <i>Clostridia</i>. These results offer basic data and theoretical support for optimizing livestock wastewater treatment systems using bacterial community structures.https://www.mdpi.com/2076-2607/9/10/2134microbial diversityswine wastewateractivated sludgewastewater quality |
| spellingShingle | Lin Shi Naiyuan Liu Gang Liu Jun Fang Bacterial Community Structure and Dynamic Changes in Different Functional Areas of a Piggery Wastewater Treatment System Microorganisms microbial diversity swine wastewater activated sludge wastewater quality |
| title | Bacterial Community Structure and Dynamic Changes in Different Functional Areas of a Piggery Wastewater Treatment System |
| title_full | Bacterial Community Structure and Dynamic Changes in Different Functional Areas of a Piggery Wastewater Treatment System |
| title_fullStr | Bacterial Community Structure and Dynamic Changes in Different Functional Areas of a Piggery Wastewater Treatment System |
| title_full_unstemmed | Bacterial Community Structure and Dynamic Changes in Different Functional Areas of a Piggery Wastewater Treatment System |
| title_short | Bacterial Community Structure and Dynamic Changes in Different Functional Areas of a Piggery Wastewater Treatment System |
| title_sort | bacterial community structure and dynamic changes in different functional areas of a piggery wastewater treatment system |
| topic | microbial diversity swine wastewater activated sludge wastewater quality |
| url | https://www.mdpi.com/2076-2607/9/10/2134 |
| work_keys_str_mv | AT linshi bacterialcommunitystructureanddynamicchangesindifferentfunctionalareasofapiggerywastewatertreatmentsystem AT naiyuanliu bacterialcommunitystructureanddynamicchangesindifferentfunctionalareasofapiggerywastewatertreatmentsystem AT gangliu bacterialcommunitystructureanddynamicchangesindifferentfunctionalareasofapiggerywastewatertreatmentsystem AT junfang bacterialcommunitystructureanddynamicchangesindifferentfunctionalareasofapiggerywastewatertreatmentsystem |