Development of Improved Process with Treatment of Cellulase for Isolation of Ampelopsin from Dried Fruits of Ampelopsis grossedentata
The commercial method for isolation of ampelopsin, one of the most common flavonoids isolated from the plant species Ampelopsis grossedentata, is a simple hydrothermal extraction at high temperature. To develop an improved process to isolate ampelopsin, the effects of treatment of cellulase on hydro...
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| Format: | Article |
| Language: | English |
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North Carolina State University
2016-02-01
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| Series: | BioResources |
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| Online Access: | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_11_1_2712_Gao_Cellulase_Isolation_Ampelopsin_Dried_Fruits |
| _version_ | 1817994939318927360 |
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| author | Wa Gao Sang-Un Lee Jianghong Li Jin-Woo Lee |
| author_facet | Wa Gao Sang-Un Lee Jianghong Li Jin-Woo Lee |
| author_sort | Wa Gao |
| collection | DOAJ |
| description | The commercial method for isolation of ampelopsin, one of the most common flavonoids isolated from the plant species Ampelopsis grossedentata, is a simple hydrothermal extraction at high temperature. To develop an improved process to isolate ampelopsin, the effects of treatment of cellulase on hydrolysis of the dried fruit of A. grossedentata were investigated. The treatment of cellulase was found to decrease the temperature and time for hydrolysis of the dried fruit of A. grossedentata. The conditions of the filter press and continuous flow centrifuge for removal of insoluble materials from the hydrolysate of the dried fruit of A. grossedentata were optimized. The recovery yield of ampelopsin from the dried fruits of A. grossedentata was 39.4%, as determined by HPLC chromatographic analysis. A safe and economical process at low temperature with treatment of cellulase for the isolation of ampelopsin was developed in this study. |
| first_indexed | 2024-04-14T01:58:38Z |
| format | Article |
| id | doaj.art-0ddb9f9907ea4853b2c550acbe39805e |
| institution | Directory Open Access Journal |
| issn | 1930-2126 1930-2126 |
| language | English |
| last_indexed | 2024-04-14T01:58:38Z |
| publishDate | 2016-02-01 |
| publisher | North Carolina State University |
| record_format | Article |
| series | BioResources |
| spelling | doaj.art-0ddb9f9907ea4853b2c550acbe39805e2022-12-22T02:18:54ZengNorth Carolina State UniversityBioResources1930-21261930-21262016-02-011112712272210.15376/biores.11.1.2712-2722Development of Improved Process with Treatment of Cellulase for Isolation of Ampelopsin from Dried Fruits of Ampelopsis grossedentataWa Gao0Sang-Un Lee1Jianghong Li2Jin-Woo Lee3Dong-A University; Korea, Republic OfDong-A University; Korea, Republic OfHuazhong Agricultural University; ChinaDong-A University; Korea, Republic OfThe commercial method for isolation of ampelopsin, one of the most common flavonoids isolated from the plant species Ampelopsis grossedentata, is a simple hydrothermal extraction at high temperature. To develop an improved process to isolate ampelopsin, the effects of treatment of cellulase on hydrolysis of the dried fruit of A. grossedentata were investigated. The treatment of cellulase was found to decrease the temperature and time for hydrolysis of the dried fruit of A. grossedentata. The conditions of the filter press and continuous flow centrifuge for removal of insoluble materials from the hydrolysate of the dried fruit of A. grossedentata were optimized. The recovery yield of ampelopsin from the dried fruits of A. grossedentata was 39.4%, as determined by HPLC chromatographic analysis. A safe and economical process at low temperature with treatment of cellulase for the isolation of ampelopsin was developed in this study.http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_11_1_2712_Gao_Cellulase_Isolation_Ampelopsin_Dried_FruitsAmpelopsinIsolationCellulaseAmpelopsis grossedentata |
| spellingShingle | Wa Gao Sang-Un Lee Jianghong Li Jin-Woo Lee Development of Improved Process with Treatment of Cellulase for Isolation of Ampelopsin from Dried Fruits of Ampelopsis grossedentata BioResources Ampelopsin Isolation Cellulase Ampelopsis grossedentata |
| title | Development of Improved Process with Treatment of Cellulase for Isolation of Ampelopsin from Dried Fruits of Ampelopsis grossedentata |
| title_full | Development of Improved Process with Treatment of Cellulase for Isolation of Ampelopsin from Dried Fruits of Ampelopsis grossedentata |
| title_fullStr | Development of Improved Process with Treatment of Cellulase for Isolation of Ampelopsin from Dried Fruits of Ampelopsis grossedentata |
| title_full_unstemmed | Development of Improved Process with Treatment of Cellulase for Isolation of Ampelopsin from Dried Fruits of Ampelopsis grossedentata |
| title_short | Development of Improved Process with Treatment of Cellulase for Isolation of Ampelopsin from Dried Fruits of Ampelopsis grossedentata |
| title_sort | development of improved process with treatment of cellulase for isolation of ampelopsin from dried fruits of ampelopsis grossedentata |
| topic | Ampelopsin Isolation Cellulase Ampelopsis grossedentata |
| url | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_11_1_2712_Gao_Cellulase_Isolation_Ampelopsin_Dried_Fruits |
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