Seqüenciamento de peptídeos usando espectrometria de massas: um guia prático Peptide sequencing using mass spectrometry: a practical guide

<abstract language="eng">This paper introduces the basics of peptide mass spectra interpretation applied to proteomics and is directed to chemists, biochemists and biologists. The manuscript presents a well detailed protocol aiming to serve as a first choice guide for understanding p...

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Bibliographic Details
Main Authors: Marcelo Delmar Cantú, Emanuel Carrilho, Nelson Arno Wulff, Mario Sérgio Palma
Format: Article
Language:English
Published: Sociedade Brasileira de Química 2008-01-01
Series:Química Nova
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422008000300034
Description
Summary:<abstract language="eng">This paper introduces the basics of peptide mass spectra interpretation applied to proteomics and is directed to chemists, biochemists and biologists. The manuscript presents a well detailed protocol aiming to serve as a first choice guide for understanding peptide sequencing. The tutorial was elaborated based on both a thorough bibliographic revision and the author's experience. In order to prove the applicability of the proposed guide, spectra obtained on different instruments have been successfully interpreted by applying the presented rational.
ISSN:0100-4042
1678-7064