Molecular and Cytogenetic Analysis of rDNA Evolution in Crepis Sensu Lato
Although Crepis was the first model plant group in which chromosomal changes were considered to play an important role in speciation, their chromosome structure and evolution have been barely investigated using molecular cytogenetic methods. The aim of the study was to provide a better understanding...
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MDPI AG
2022-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/7/3643 |
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| author | Magdalena Senderowicz Teresa Nowak Hanna Weiss-Schneeweiss Laszlo Papp Bozena Kolano |
| author_facet | Magdalena Senderowicz Teresa Nowak Hanna Weiss-Schneeweiss Laszlo Papp Bozena Kolano |
| author_sort | Magdalena Senderowicz |
| collection | DOAJ |
| description | Although Crepis was the first model plant group in which chromosomal changes were considered to play an important role in speciation, their chromosome structure and evolution have been barely investigated using molecular cytogenetic methods. The aim of the study was to provide a better understanding of the patterns and directions of Crepis chromosome evolution, using comparative analyses of rDNA loci number and localisation. The chromosome base number and chromosomal organisation of 5S and 35S rDNA loci were analysed in the phylogenetic background for 39 species of Crepis, which represent the evolutionary lineages of Crepis sensu stricto and Lagoseris, including <i>Lapsana communis</i>. The phylogenetic relationships among all the species were inferred from nrITS and newly obtained 5S rDNA NTS sequences. Despite high variations in rDNA loci chromosomal organisation, most species had a chromosome with both rDNA loci within the same (usually short) chromosomal arm. The comparative analyses revealed several independent rDNA loci number gains and loci repositioning that accompanied diversification and speciation in Crepis. Some of the changes in rDNA loci patterns were reconstructed for the same evolutionary lineages as descending dysploidy. |
| first_indexed | 2024-03-09T11:47:08Z |
| format | Article |
| id | doaj.art-0ddcfe4a211b4890a3e11f7e00fe147d |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T11:47:08Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-0ddcfe4a211b4890a3e11f7e00fe147d2023-11-30T23:19:56ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-03-01237364310.3390/ijms23073643Molecular and Cytogenetic Analysis of rDNA Evolution in Crepis Sensu LatoMagdalena Senderowicz0Teresa Nowak1Hanna Weiss-Schneeweiss2Laszlo Papp3Bozena Kolano4Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, 40-007 Katowice, PolandInstitute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, 40-007 Katowice, PolandDepartment of Botany and Biodiversity Research, University of Vienna, Rennweg 14, A-1030 Vienna, AustriaEötvös Loránd University Botanical Garden, Illés u. 25, 1083 Budapest, HungaryInstitute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, 40-007 Katowice, PolandAlthough Crepis was the first model plant group in which chromosomal changes were considered to play an important role in speciation, their chromosome structure and evolution have been barely investigated using molecular cytogenetic methods. The aim of the study was to provide a better understanding of the patterns and directions of Crepis chromosome evolution, using comparative analyses of rDNA loci number and localisation. The chromosome base number and chromosomal organisation of 5S and 35S rDNA loci were analysed in the phylogenetic background for 39 species of Crepis, which represent the evolutionary lineages of Crepis sensu stricto and Lagoseris, including <i>Lapsana communis</i>. The phylogenetic relationships among all the species were inferred from nrITS and newly obtained 5S rDNA NTS sequences. Despite high variations in rDNA loci chromosomal organisation, most species had a chromosome with both rDNA loci within the same (usually short) chromosomal arm. The comparative analyses revealed several independent rDNA loci number gains and loci repositioning that accompanied diversification and speciation in Crepis. Some of the changes in rDNA loci patterns were reconstructed for the same evolutionary lineages as descending dysploidy.https://www.mdpi.com/1422-0067/23/7/3643rDNA lociCrepis5S rDNA NTSnrITSchromosomesFISH |
| spellingShingle | Magdalena Senderowicz Teresa Nowak Hanna Weiss-Schneeweiss Laszlo Papp Bozena Kolano Molecular and Cytogenetic Analysis of rDNA Evolution in Crepis Sensu Lato International Journal of Molecular Sciences rDNA loci Crepis 5S rDNA NTS nrITS chromosomes FISH |
| title | Molecular and Cytogenetic Analysis of rDNA Evolution in Crepis Sensu Lato |
| title_full | Molecular and Cytogenetic Analysis of rDNA Evolution in Crepis Sensu Lato |
| title_fullStr | Molecular and Cytogenetic Analysis of rDNA Evolution in Crepis Sensu Lato |
| title_full_unstemmed | Molecular and Cytogenetic Analysis of rDNA Evolution in Crepis Sensu Lato |
| title_short | Molecular and Cytogenetic Analysis of rDNA Evolution in Crepis Sensu Lato |
| title_sort | molecular and cytogenetic analysis of rdna evolution in crepis sensu lato |
| topic | rDNA loci Crepis 5S rDNA NTS nrITS chromosomes FISH |
| url | https://www.mdpi.com/1422-0067/23/7/3643 |
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