DNA display I. Sequence-encoded routing of DNA populations.
Recently reported technologies for DNA-directed organic synthesis and for DNA computing rely on routing DNA populations through complex networks. The reduction of these ideas to practice has been limited by a lack of practical experimental tools. Here we describe a modular design for DNA routing gen...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2004-07-01
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| Series: | PLoS Biology |
| Online Access: | https://doi.org/10.1371/journal.pbio.0020173 |
| _version_ | 1819021064100904960 |
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| author | David R Halpin Pehr B Harbury |
| author_facet | David R Halpin Pehr B Harbury |
| author_sort | David R Halpin |
| collection | DOAJ |
| description | Recently reported technologies for DNA-directed organic synthesis and for DNA computing rely on routing DNA populations through complex networks. The reduction of these ideas to practice has been limited by a lack of practical experimental tools. Here we describe a modular design for DNA routing genes, and routing machinery made from oligonucleotides and commercially available chromatography resins. The routing machinery partitions nanomole quantities of DNA into physically distinct subpools based on sequence. Partitioning steps can be iterated indefinitely, with worst-case yields of 85% per step. These techniques facilitate DNA-programmed chemical synthesis, and thus enable a materials biology that could revolutionize drug discovery. |
| first_indexed | 2024-12-21T04:01:09Z |
| format | Article |
| id | doaj.art-0de05a8065f54e97b98d5e37c1f07a5b |
| institution | Directory Open Access Journal |
| issn | 1544-9173 1545-7885 |
| language | English |
| last_indexed | 2024-12-21T04:01:09Z |
| publishDate | 2004-07-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Biology |
| spelling | doaj.art-0de05a8065f54e97b98d5e37c1f07a5b2022-12-21T19:16:43ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852004-07-0127E17310.1371/journal.pbio.0020173DNA display I. Sequence-encoded routing of DNA populations.David R HalpinPehr B HarburyRecently reported technologies for DNA-directed organic synthesis and for DNA computing rely on routing DNA populations through complex networks. The reduction of these ideas to practice has been limited by a lack of practical experimental tools. Here we describe a modular design for DNA routing genes, and routing machinery made from oligonucleotides and commercially available chromatography resins. The routing machinery partitions nanomole quantities of DNA into physically distinct subpools based on sequence. Partitioning steps can be iterated indefinitely, with worst-case yields of 85% per step. These techniques facilitate DNA-programmed chemical synthesis, and thus enable a materials biology that could revolutionize drug discovery.https://doi.org/10.1371/journal.pbio.0020173 |
| spellingShingle | David R Halpin Pehr B Harbury DNA display I. Sequence-encoded routing of DNA populations. PLoS Biology |
| title | DNA display I. Sequence-encoded routing of DNA populations. |
| title_full | DNA display I. Sequence-encoded routing of DNA populations. |
| title_fullStr | DNA display I. Sequence-encoded routing of DNA populations. |
| title_full_unstemmed | DNA display I. Sequence-encoded routing of DNA populations. |
| title_short | DNA display I. Sequence-encoded routing of DNA populations. |
| title_sort | dna display i sequence encoded routing of dna populations |
| url | https://doi.org/10.1371/journal.pbio.0020173 |
| work_keys_str_mv | AT davidrhalpin dnadisplayisequenceencodedroutingofdnapopulations AT pehrbharbury dnadisplayisequenceencodedroutingofdnapopulations |