Identification of mcr-1-positive multidrug-resistant Escherichia coli isolates from clinical samples in Shanghai, China
ABSTRACT: Objectives: Since the gene encoding mobilised colistin resistance (mcr-1) was first reported in China in 2015, it has been reported in various Enterobacteriaceae worldwide. Escherichia coli, one of the main pathogens causing diarrhoea, is the most prevalent, clinically identified species...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | Journal of Global Antimicrobial Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S221371652200042X |
| _version_ | 1818230791138705408 |
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| author | Jing Xie Beibei Liang Xuebin Xu Lang Yang Huan Li Peng Li Shaofu Qiu Hongbin Song |
| author_facet | Jing Xie Beibei Liang Xuebin Xu Lang Yang Huan Li Peng Li Shaofu Qiu Hongbin Song |
| author_sort | Jing Xie |
| collection | DOAJ |
| description | ABSTRACT: Objectives: Since the gene encoding mobilised colistin resistance (mcr-1) was first reported in China in 2015, it has been reported in various Enterobacteriaceae worldwide. Escherichia coli, one of the main pathogens causing diarrhoea, is the most prevalent, clinically identified species carrying mcr-1. This study aimed to investigate the epidemiologic and genomic characteristics of mcr-1 in Escherichia coli from patients in Shanghai. Methods: Faecal samples were collected from hospitals in Shanghai between 2012 and 2015. Polymerase chain reaction was performed to detect mcr-1, and molecular characteristics of the mcr-1-positive E. coli was determined by antimicrobial susceptibility testing and whole-genome sequencing. Results: We detected 40 (3.9%) mcr-1-positive E. coli strains from faecal samples in clinical settings between 2012 and 2015 in Shanghai. Mcr-1 was detected in 4 types of E. coli, including atypical enteropathogenic E. coli (aEPEC), enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC), and enteroaggregative E. coli (EAEC). Most strains harbouring mcr-1 were isolated from children aged <7 years. Whole-genome sequencing revealed that nearly half of the strains that carried quinolone resistance- or β-lactam resistance-related genes were multidrug-resistant. IncX4 was the predominant type in mcr-1-positive E. coli in Shanghai, but the other types of mcr-1-harbouring plasmids are highly diverse in genetic context. Conclusion: These data suggest that mcr-1 is prevalent in E. coli strains, especially those identified in diarrhoeal patients in Shanghai, and strengthening the surveillance of mcr-1 transmission, especially in children, is essential. |
| first_indexed | 2024-12-12T10:40:06Z |
| format | Article |
| id | doaj.art-0de0abc402e9421b89b3d0f8d64ea841 |
| institution | Directory Open Access Journal |
| issn | 2213-7165 |
| language | English |
| last_indexed | 2024-12-12T10:40:06Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj.art-0de0abc402e9421b89b3d0f8d64ea8412022-12-22T00:27:04ZengElsevierJournal of Global Antimicrobial Resistance2213-71652022-06-01298896Identification of mcr-1-positive multidrug-resistant Escherichia coli isolates from clinical samples in Shanghai, ChinaJing Xie0Beibei Liang1Xuebin Xu2Lang Yang3Huan Li4Peng Li5Shaofu Qiu6Hongbin Song7Center for Disease Control and Prevention of PLA, Beijing, ChinaCenter for Disease Control and Prevention of PLA, Beijing, China; School and Hospital of Stomatology, Hebei Medical University, ChinaShanghai Municipal Center for Disease Control and Prevention, Shanghai, ChinaCenter for Disease Control and Prevention of PLA, Beijing, ChinaCenter for Disease Control and Prevention of PLA, Beijing, ChinaCenter for Disease Control and Prevention of PLA, Beijing, ChinaCenter for Disease Control and Prevention of PLA, Beijing, ChinaCenter for Disease Control and Prevention of PLA, Beijing, China; Corresponding author. Center for Disease Control and Prevention of PLA, 20 Dongda Street, Fengtai District, Beijing 100071, China.ABSTRACT: Objectives: Since the gene encoding mobilised colistin resistance (mcr-1) was first reported in China in 2015, it has been reported in various Enterobacteriaceae worldwide. Escherichia coli, one of the main pathogens causing diarrhoea, is the most prevalent, clinically identified species carrying mcr-1. This study aimed to investigate the epidemiologic and genomic characteristics of mcr-1 in Escherichia coli from patients in Shanghai. Methods: Faecal samples were collected from hospitals in Shanghai between 2012 and 2015. Polymerase chain reaction was performed to detect mcr-1, and molecular characteristics of the mcr-1-positive E. coli was determined by antimicrobial susceptibility testing and whole-genome sequencing. Results: We detected 40 (3.9%) mcr-1-positive E. coli strains from faecal samples in clinical settings between 2012 and 2015 in Shanghai. Mcr-1 was detected in 4 types of E. coli, including atypical enteropathogenic E. coli (aEPEC), enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC), and enteroaggregative E. coli (EAEC). Most strains harbouring mcr-1 were isolated from children aged <7 years. Whole-genome sequencing revealed that nearly half of the strains that carried quinolone resistance- or β-lactam resistance-related genes were multidrug-resistant. IncX4 was the predominant type in mcr-1-positive E. coli in Shanghai, but the other types of mcr-1-harbouring plasmids are highly diverse in genetic context. Conclusion: These data suggest that mcr-1 is prevalent in E. coli strains, especially those identified in diarrhoeal patients in Shanghai, and strengthening the surveillance of mcr-1 transmission, especially in children, is essential.http://www.sciencedirect.com/science/article/pii/S221371652200042Xmcr-1E. coliFaecal samplesMultidrug-resistant bacteriaShanghai |
| spellingShingle | Jing Xie Beibei Liang Xuebin Xu Lang Yang Huan Li Peng Li Shaofu Qiu Hongbin Song Identification of mcr-1-positive multidrug-resistant Escherichia coli isolates from clinical samples in Shanghai, China Journal of Global Antimicrobial Resistance mcr-1 E. coli Faecal samples Multidrug-resistant bacteria Shanghai |
| title | Identification of mcr-1-positive multidrug-resistant Escherichia coli isolates from clinical samples in Shanghai, China |
| title_full | Identification of mcr-1-positive multidrug-resistant Escherichia coli isolates from clinical samples in Shanghai, China |
| title_fullStr | Identification of mcr-1-positive multidrug-resistant Escherichia coli isolates from clinical samples in Shanghai, China |
| title_full_unstemmed | Identification of mcr-1-positive multidrug-resistant Escherichia coli isolates from clinical samples in Shanghai, China |
| title_short | Identification of mcr-1-positive multidrug-resistant Escherichia coli isolates from clinical samples in Shanghai, China |
| title_sort | identification of mcr 1 positive multidrug resistant escherichia coli isolates from clinical samples in shanghai china |
| topic | mcr-1 E. coli Faecal samples Multidrug-resistant bacteria Shanghai |
| url | http://www.sciencedirect.com/science/article/pii/S221371652200042X |
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