电感耦合等离子体质谱法测定蛋黄卵磷脂中15种金属元素Determination of 15 kinds of metal elements in egg yolk lecithin by inductively coupled plasma mass spectrometry
蛋黄卵磷脂样品经微波消解，采用电感耦合等离子体质谱法测定样品中15种金属元素含量，对检测条件进行了优化，并进行了方法学考察。结果表明，在射频功率1.55 kW、载气（高纯氩气）流速 1.03 L/min、等离子气体流速15.0 L/min、蠕动泵转速 0.1 r/s、采样深度 10 mm、重复次数 3 次条件下，通过在线加入内标钪(Sc)、锗(Ge)、铟(In)、铼(Re)元素可校正基体效应和干扰，测定汞元素时，加入金元素可降低测定误差；该方法的15 种金属元素线性关系良好，线性相关系数（R）均大于0.999；方法检出限在0.03～0.65 ng/mL之间，定量限在0.10～1.97 ng/m...
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中粮工科（西安）国际工程有限公司
2022-02-01
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| Series: | Zhongguo youzhi |
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| Online Access: | http://tg.chinaoils.cn/ch/reader/create_pdf.aspx?file_no=20220223&year_id=2022&quarter_id=2&falg=1 |
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| author | 陈秋玲，祝芷琦，李咏华，王小妹，梁北梅，温恺嘉，许一靖，曾还雄，林元亨，唐顺之 CHEN Qiuling, ZHU Zhiqi, LI Yonghua,WANG Xiaomei,LIANG Beimei, WEN Kaijia,XU Yijing,ZENG Huanxiong,LIN Yuanheng,TANG Shunzhi |
| author_facet | 陈秋玲，祝芷琦，李咏华，王小妹，梁北梅，温恺嘉，许一靖，曾还雄，林元亨，唐顺之 CHEN Qiuling, ZHU Zhiqi, LI Yonghua,WANG Xiaomei,LIANG Beimei, WEN Kaijia,XU Yijing,ZENG Huanxiong,LIN Yuanheng,TANG Shunzhi |
| author_sort | 陈秋玲，祝芷琦，李咏华，王小妹，梁北梅，温恺嘉，许一靖，曾还雄，林元亨，唐顺之 CHEN Qiuling, ZHU Zhiqi, LI Yonghua,WANG Xiaomei,LIANG Beimei, WEN Kaijia,XU Yijing,ZENG Huanxiong,LIN Yuanheng,TANG Shunzhi |
| collection | DOAJ |
| description | 蛋黄卵磷脂样品经微波消解，采用电感耦合等离子体质谱法测定样品中15种金属元素含量，对检测条件进行了优化，并进行了方法学考察。结果表明，在射频功率1.55 kW、载气（高纯氩气）流速 1.03 L/min、等离子气体流速15.0 L/min、蠕动泵转速 0.1 r/s、采样深度 10 mm、重复次数 3 次条件下，通过在线加入内标钪(Sc)、锗(Ge)、铟(In)、铼(Re)元素可校正基体效应和干扰，测定汞元素时，加入金元素可降低测定误差；该方法的15 种金属元素线性关系良好，线性相关系数（R）均大于0.999；方法检出限在0.03～0.65 ng/mL之间，定量限在0.10～1.97 ng/mL之间；仪器精密度良好，RSD在0.45%～4.67%之间；重复性RSD在1.78%～8.72%之间；中间精密度RSD在2.24%～9.45%之间；加标回收率在92.67%～132.90%之间, RSD在1.42%～7.00%之间。样品溶液在2~8 ℃条件下保存72 h，稳定性良好，RSD在0.56%～3.93%之间。该方法快速、准确、灵敏度高, 可用于蛋黄卵磷脂中15种金属元素的同时测定。
Egg yolk lecithin samples were digested by microwave and 15 kinds of metal elements in it were determined by inductively coupled plasma mass spectrometry(ICP-MS). The detection conditions were optimized and the methodology was investigated. The results showed that under the conditions of RF power 1.55 kW, flow rate of carrier gas(high purity argon)1.03 L/min, flow rate of plasma gas 15.0 L/min, peristaltic pump speed 0.1 r/s, sampling depth 10 mm and 3 replications, the internal standard solution with Sc，Ge，In and Re were added online for matrix effect and interference correction, and Au was added when determining the content of Hg to reduce the test error. The linearities for the 15 elements of the method were good with the correlation coefficients（R）above 0.999. The detection limit and quantitation limit of 15 elements were 0.03-0.65 ng/mL and 0.10-1.97 ng/mL, respectively. The precisions of the instrument were good with RSD in the range of 0.45%-4.67%. The RSD of the repeatability test was in the range of 1.78%-8.72%.The RSD of the intermediate precision was in the range of 2.24%-9.45%. The recovery rate of the 15 elements was in the range of 92.67%-132.90% with RSD in the range of 1.42%-7.00%. The stability of the sample solution was good and when it was stored at 2-8 ℃ for 72 h, RSD was in the range of 0.56%-3.93%. The method had advantages of fastness, accuracy and high sensitiveness, and it could be used for simultaneous determination of 15 kinds of metal elements in egg yolk lecithin. |
| first_indexed | 2024-03-12T17:03:33Z |
| format | Article |
| id | doaj.art-0de4d6b4b84b4ff8b5e61cfdc9749ff8 |
| institution | Directory Open Access Journal |
| issn | 1003-7969 |
| language | English |
| last_indexed | 2024-03-12T17:03:33Z |
| publishDate | 2022-02-01 |
| publisher | 中粮工科（西安）国际工程有限公司 |
| record_format | Article |
| series | Zhongguo youzhi |
| spelling | doaj.art-0de4d6b4b84b4ff8b5e61cfdc9749ff82023-08-07T06:39:15Zeng中粮工科（西安）国际工程有限公司Zhongguo youzhi1003-79692022-02-0147212913510.19902/j.cnki.zgyz.1003-7969.210060电感耦合等离子体质谱法测定蛋黄卵磷脂中15种金属元素Determination of 15 kinds of metal elements in egg yolk lecithin by inductively coupled plasma mass spectrometry陈秋玲，祝芷琦，李咏华，王小妹，梁北梅，温恺嘉，许一靖，曾还雄，林元亨，唐顺之 CHEN Qiuling, ZHU Zhiqi, LI Yonghua,WANG Xiaomei,LIANG Beimei, WEN Kaijia,XU Yijing,ZENG Huanxiong,LIN Yuanheng,TANG Shunzhi0广州白云山汉方现代药业有限公司 中药提取分离过程现代化国家工程研究中心， 广东省药用脂质重点实验室， 广州 510240(Guangdong Key Laboratory of Medicinal Lipids，National Engineering Research Center (NERC) for Modernization of Extraction and Separation Process of TCM, Guangzhou Hanfang Pharmaceutical Co.,Ltd.，Guangzhou 510240, China蛋黄卵磷脂样品经微波消解，采用电感耦合等离子体质谱法测定样品中15种金属元素含量，对检测条件进行了优化，并进行了方法学考察。结果表明，在射频功率1.55 kW、载气（高纯氩气）流速 1.03 L/min、等离子气体流速15.0 L/min、蠕动泵转速 0.1 r/s、采样深度 10 mm、重复次数 3 次条件下，通过在线加入内标钪(Sc)、锗(Ge)、铟(In)、铼(Re)元素可校正基体效应和干扰，测定汞元素时，加入金元素可降低测定误差；该方法的15 种金属元素线性关系良好，线性相关系数（R）均大于0.999；方法检出限在0.03～0.65 ng/mL之间，定量限在0.10～1.97 ng/mL之间；仪器精密度良好，RSD在0.45%～4.67%之间；重复性RSD在1.78%～8.72%之间；中间精密度RSD在2.24%～9.45%之间；加标回收率在92.67%～132.90%之间, RSD在1.42%～7.00%之间。样品溶液在2~8 ℃条件下保存72 h，稳定性良好，RSD在0.56%～3.93%之间。该方法快速、准确、灵敏度高, 可用于蛋黄卵磷脂中15种金属元素的同时测定。 Egg yolk lecithin samples were digested by microwave and 15 kinds of metal elements in it were determined by inductively coupled plasma mass spectrometry(ICP-MS). The detection conditions were optimized and the methodology was investigated. The results showed that under the conditions of RF power 1.55 kW, flow rate of carrier gas(high purity argon)1.03 L/min, flow rate of plasma gas 15.0 L/min, peristaltic pump speed 0.1 r/s, sampling depth 10 mm and 3 replications, the internal standard solution with Sc，Ge，In and Re were added online for matrix effect and interference correction, and Au was added when determining the content of Hg to reduce the test error. The linearities for the 15 elements of the method were good with the correlation coefficients（R）above 0.999. The detection limit and quantitation limit of 15 elements were 0.03-0.65 ng/mL and 0.10-1.97 ng/mL, respectively. The precisions of the instrument were good with RSD in the range of 0.45%-4.67%. The RSD of the repeatability test was in the range of 1.78%-8.72%.The RSD of the intermediate precision was in the range of 2.24%-9.45%. The recovery rate of the 15 elements was in the range of 92.67%-132.90% with RSD in the range of 1.42%-7.00%. The stability of the sample solution was good and when it was stored at 2-8 ℃ for 72 h, RSD was in the range of 0.56%-3.93%. The method had advantages of fastness, accuracy and high sensitiveness, and it could be used for simultaneous determination of 15 kinds of metal elements in egg yolk lecithin.http://tg.chinaoils.cn/ch/reader/create_pdf.aspx?file_no=20220223&year_id=2022&quarter_id=2&falg=1蛋黄卵磷脂; 电感耦合等离子体质谱法; 微波消解; 金属元素egg yolk lecithin; inductively coupled plasma-mass spectrometry; microwave digestion; metal element |
| spellingShingle | 陈秋玲，祝芷琦，李咏华，王小妹，梁北梅，温恺嘉，许一靖，曾还雄，林元亨，唐顺之 CHEN Qiuling, ZHU Zhiqi, LI Yonghua,WANG Xiaomei,LIANG Beimei, WEN Kaijia,XU Yijing,ZENG Huanxiong,LIN Yuanheng,TANG Shunzhi 电感耦合等离子体质谱法测定蛋黄卵磷脂中15种金属元素Determination of 15 kinds of metal elements in egg yolk lecithin by inductively coupled plasma mass spectrometry Zhongguo youzhi 蛋黄卵磷脂; 电感耦合等离子体质谱法; 微波消解; 金属元素 egg yolk lecithin; inductively coupled plasma-mass spectrometry; microwave digestion; metal element |
| title | 电感耦合等离子体质谱法测定蛋黄卵磷脂中15种金属元素Determination of 15 kinds of metal elements in egg yolk lecithin by inductively coupled plasma mass spectrometry |
| title_full | 电感耦合等离子体质谱法测定蛋黄卵磷脂中15种金属元素Determination of 15 kinds of metal elements in egg yolk lecithin by inductively coupled plasma mass spectrometry |
| title_fullStr | 电感耦合等离子体质谱法测定蛋黄卵磷脂中15种金属元素Determination of 15 kinds of metal elements in egg yolk lecithin by inductively coupled plasma mass spectrometry |
| title_full_unstemmed | 电感耦合等离子体质谱法测定蛋黄卵磷脂中15种金属元素Determination of 15 kinds of metal elements in egg yolk lecithin by inductively coupled plasma mass spectrometry |
| title_short | 电感耦合等离子体质谱法测定蛋黄卵磷脂中15种金属元素Determination of 15 kinds of metal elements in egg yolk lecithin by inductively coupled plasma mass spectrometry |
| title_sort | 电感耦合等离子体质谱法测定蛋黄卵磷脂中15种金属元素determination of 15 kinds of metal elements in egg yolk lecithin by inductively coupled plasma mass spectrometry |
| topic | 蛋黄卵磷脂; 电感耦合等离子体质谱法; 微波消解; 金属元素 egg yolk lecithin; inductively coupled plasma-mass spectrometry; microwave digestion; metal element |
| url | http://tg.chinaoils.cn/ch/reader/create_pdf.aspx?file_no=20220223&year_id=2022&quarter_id=2&falg=1 |
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