Evaluation of the efficacy of different intracanal medicaments against Candida albicans and Enterococcus faecalis - An In-Vitro study
Aim: This study aims to evaluate the antimicrobial efficacy of certain intracanal medicaments against Candida albicans and Enterococcus faecalis. Methods: Freshly extracted 120 single rooted mandibular premolars were selected for the study. Teeth were decoronated and cleaning and shaping was done up...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2022-01-01
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| Series: | Indian Journal of Dental Research |
| Subjects: | |
| Online Access: | http://www.ijdr.in/article.asp?issn=0970-9290;year=2022;volume=33;issue=4;spage=440;epage=444;aulast=Mathew |
| _version_ | 1827944892801744896 |
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| author | Rohit Mathew Ashok S Sukumaran Prabath Singh Anju V Varughese |
| author_facet | Rohit Mathew Ashok S Sukumaran Prabath Singh Anju V Varughese |
| author_sort | Rohit Mathew |
| collection | DOAJ |
| description | Aim: This study aims to evaluate the antimicrobial efficacy of certain intracanal medicaments against Candida albicans and Enterococcus faecalis. Methods: Freshly extracted 120 single rooted mandibular premolars were selected for the study. Teeth were decoronated and cleaning and shaping was done up to F3 universal protaper system and were divided mainly into two groups: Candida albicans (C. albicans) (n = 60) and Enterococcus faecalis (E. faecalis) (n = 60). The medicaments include: G1: chlorhexidine + calcium hydroxide, G2: sodium hypochlorite + calcium hydroxide, G3: 2% chlorhexidine gel, G4: octenisept, G5: 0.1% octenisept solution + calcium hydroxide, and G6: physiologic saline (n = 5). Teeth were contaminated with Enterococcus faecalis and Candida albicans which were cultured, respectively, in brain heart infusion and Sabouraud's dextrose agar for 21 days followed by intracanal medication and colony forming units were counted on the second and seventh day. Statistical analysis was done using Analysis of Variance (ANOVA) and Tukey's post hoc test. Results: Against C. albicans, CHX + CH, 2% CHX gel, 0.1% octenidine (OCT) gel and OCT + CH showed statistically significant differences on the 2nd and 7th day. But against Enterococcus faecalis, only 0.1% OCT gel and 2% CHX gel showed statistically significant differences on the 2nd and 7th day. Among all the groups, 0.1% OCT gel and 2% CHX gel showed predominant antimicrobial efficacy. Conclusion: From the limitations of the current study, all the medicaments showed antimicrobial effect against Candida albicans and Enterococcus faecalis on the 2nd and 7th day with higher microbial inhibition on the 7th day. |
| first_indexed | 2024-03-13T10:37:41Z |
| format | Article |
| id | doaj.art-0de6913ba3bf4f2096fe1ba042b80d20 |
| institution | Directory Open Access Journal |
| issn | 0970-9290 1998-3603 |
| language | English |
| last_indexed | 2024-03-13T10:37:41Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Indian Journal of Dental Research |
| spelling | doaj.art-0de6913ba3bf4f2096fe1ba042b80d202023-05-18T05:27:17ZengWolters Kluwer Medknow PublicationsIndian Journal of Dental Research0970-92901998-36032022-01-0133444044410.4103/ijdr.ijdr_10_22Evaluation of the efficacy of different intracanal medicaments against Candida albicans and Enterococcus faecalis - An In-Vitro studyRohit MathewAshok S SukumaranPrabath SinghAnju V VarugheseAim: This study aims to evaluate the antimicrobial efficacy of certain intracanal medicaments against Candida albicans and Enterococcus faecalis. Methods: Freshly extracted 120 single rooted mandibular premolars were selected for the study. Teeth were decoronated and cleaning and shaping was done up to F3 universal protaper system and were divided mainly into two groups: Candida albicans (C. albicans) (n = 60) and Enterococcus faecalis (E. faecalis) (n = 60). The medicaments include: G1: chlorhexidine + calcium hydroxide, G2: sodium hypochlorite + calcium hydroxide, G3: 2% chlorhexidine gel, G4: octenisept, G5: 0.1% octenisept solution + calcium hydroxide, and G6: physiologic saline (n = 5). Teeth were contaminated with Enterococcus faecalis and Candida albicans which were cultured, respectively, in brain heart infusion and Sabouraud's dextrose agar for 21 days followed by intracanal medication and colony forming units were counted on the second and seventh day. Statistical analysis was done using Analysis of Variance (ANOVA) and Tukey's post hoc test. Results: Against C. albicans, CHX + CH, 2% CHX gel, 0.1% octenidine (OCT) gel and OCT + CH showed statistically significant differences on the 2nd and 7th day. But against Enterococcus faecalis, only 0.1% OCT gel and 2% CHX gel showed statistically significant differences on the 2nd and 7th day. Among all the groups, 0.1% OCT gel and 2% CHX gel showed predominant antimicrobial efficacy. Conclusion: From the limitations of the current study, all the medicaments showed antimicrobial effect against Candida albicans and Enterococcus faecalis on the 2nd and 7th day with higher microbial inhibition on the 7th day.http://www.ijdr.in/article.asp?issn=0970-9290;year=2022;volume=33;issue=4;spage=440;epage=444;aulast=Mathewcalcium hydroxidecandida albicanschlorhexidineenterococcus faecalisoctenidinesodium hypochlorite |
| spellingShingle | Rohit Mathew Ashok S Sukumaran Prabath Singh Anju V Varughese Evaluation of the efficacy of different intracanal medicaments against Candida albicans and Enterococcus faecalis - An In-Vitro study Indian Journal of Dental Research calcium hydroxide candida albicans chlorhexidine enterococcus faecalis octenidine sodium hypochlorite |
| title | Evaluation of the efficacy of different intracanal medicaments against Candida albicans and Enterococcus faecalis - An In-Vitro study |
| title_full | Evaluation of the efficacy of different intracanal medicaments against Candida albicans and Enterococcus faecalis - An In-Vitro study |
| title_fullStr | Evaluation of the efficacy of different intracanal medicaments against Candida albicans and Enterococcus faecalis - An In-Vitro study |
| title_full_unstemmed | Evaluation of the efficacy of different intracanal medicaments against Candida albicans and Enterococcus faecalis - An In-Vitro study |
| title_short | Evaluation of the efficacy of different intracanal medicaments against Candida albicans and Enterococcus faecalis - An In-Vitro study |
| title_sort | evaluation of the efficacy of different intracanal medicaments against candida albicans and enterococcus faecalis an in vitro study |
| topic | calcium hydroxide candida albicans chlorhexidine enterococcus faecalis octenidine sodium hypochlorite |
| url | http://www.ijdr.in/article.asp?issn=0970-9290;year=2022;volume=33;issue=4;spage=440;epage=444;aulast=Mathew |
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