Genome-Wide Characterization of the Mitogen-Activated Protein Kinase Gene Family and Their Expression Patterns in Response to Drought and Colletotrichum Gloeosporioides in Walnut (<i>Juglans regia</i>)
Mitogen-activated protein kinases (MAPKs) are a family of Ser/Thr (serine/threonine) protein kinases that play very important roles in plant responses to biotic and abiotic stressors. However, the MAPK gene family in the important crop walnut (<i>Juglans regia</i> L.) has been less well...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-01-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/12/3/586 |
| _version_ | 1797623443488768000 |
|---|---|
| author | Kaiyu Yang Jianghao Wu Xialei Wang Han Li Peng Jia Haoan Luan Xuemei Zhang Suping Guo Minsheng Yang Qinglong Dong Guohui Qi |
| author_facet | Kaiyu Yang Jianghao Wu Xialei Wang Han Li Peng Jia Haoan Luan Xuemei Zhang Suping Guo Minsheng Yang Qinglong Dong Guohui Qi |
| author_sort | Kaiyu Yang |
| collection | DOAJ |
| description | Mitogen-activated protein kinases (MAPKs) are a family of Ser/Thr (serine/threonine) protein kinases that play very important roles in plant responses to biotic and abiotic stressors. However, the MAPK gene family in the important crop walnut (<i>Juglans regia</i> L.) has been less well studied compared with other species. We discovered 25 <i>JrMAPK</i> members in the <i>Juglans</i> genome in this study. The <i>JrMAPK</i> gene family was separated into four subfamilies based on phylogenetic analysis, and members of the same subgroup had similar motifs and exons/introns. A variety of cis-acting elements, mainly related to the light response, growth and development, stress response, and hormone responses, were detected in the <i>JrMAPK</i> gene promoters. Collinearity analysis showed that purification selection was the main driving force in <i>JrMAPK</i> gene evolution, and segmental and tandem duplications played key roles in the expansion of the <i>JrMAPK</i> gene family. The RNA-Seq (RNA Sequencing) results indicated that many of the <i>JrMAPK</i> genes were expressed in response to different levels of <i>Colletotrichum gloeosporioides</i> infection. <i>JrMAPK1</i>, <i>JrMAPK3</i>, <i>JrMAPK4</i>, <i>JrMAPK5</i>, <i>JrMAPK6</i>, <i>JrMAPK7</i>, <i>JrMAPK9</i>, <i>JrMAPK11</i>, <i>JrMAPK12</i>, <i>JrMAPK13</i>, <i>JrMAPK17</i>, <i>JrMAPK19</i>, <i>JrMAPK20</i>, and <i>JrMAPK21</i> were upregulated at the transcriptional level in response to the drought stress treatment. The results of this study will help in further investigations of the evolutionary history and biological functions of the MAPK gene family in walnut. |
| first_indexed | 2024-03-11T09:29:04Z |
| format | Article |
| id | doaj.art-0dea1b4a13424057929169f1e81edea4 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T09:29:04Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-0dea1b4a13424057929169f1e81edea42023-11-16T17:44:34ZengMDPI AGPlants2223-77472023-01-0112358610.3390/plants12030586Genome-Wide Characterization of the Mitogen-Activated Protein Kinase Gene Family and Their Expression Patterns in Response to Drought and Colletotrichum Gloeosporioides in Walnut (<i>Juglans regia</i>)Kaiyu Yang0Jianghao Wu1Xialei Wang2Han Li3Peng Jia4Haoan Luan5Xuemei Zhang6Suping Guo7Minsheng Yang8Qinglong Dong9Guohui Qi10College of Forestry, Hebei Agricultural University, Baoding 071001, ChinaCollege of Forestry, Hebei Agricultural University, Baoding 071001, ChinaCollege of Forestry, Hebei Agricultural University, Baoding 071001, ChinaCollege of Forestry, Hebei Agricultural University, Baoding 071001, ChinaCollege of Forestry, Hebei Agricultural University, Baoding 071001, ChinaCollege of Forestry, Hebei Agricultural University, Baoding 071001, ChinaCollege of Forestry, Hebei Agricultural University, Baoding 071001, ChinaCollege of Forestry, Hebei Agricultural University, Baoding 071001, ChinaCollege of Forestry, Hebei Agricultural University, Baoding 071001, ChinaCollege of Forestry, Hebei Agricultural University, Baoding 071001, ChinaCollege of Forestry, Hebei Agricultural University, Baoding 071001, ChinaMitogen-activated protein kinases (MAPKs) are a family of Ser/Thr (serine/threonine) protein kinases that play very important roles in plant responses to biotic and abiotic stressors. However, the MAPK gene family in the important crop walnut (<i>Juglans regia</i> L.) has been less well studied compared with other species. We discovered 25 <i>JrMAPK</i> members in the <i>Juglans</i> genome in this study. The <i>JrMAPK</i> gene family was separated into four subfamilies based on phylogenetic analysis, and members of the same subgroup had similar motifs and exons/introns. A variety of cis-acting elements, mainly related to the light response, growth and development, stress response, and hormone responses, were detected in the <i>JrMAPK</i> gene promoters. Collinearity analysis showed that purification selection was the main driving force in <i>JrMAPK</i> gene evolution, and segmental and tandem duplications played key roles in the expansion of the <i>JrMAPK</i> gene family. The RNA-Seq (RNA Sequencing) results indicated that many of the <i>JrMAPK</i> genes were expressed in response to different levels of <i>Colletotrichum gloeosporioides</i> infection. <i>JrMAPK1</i>, <i>JrMAPK3</i>, <i>JrMAPK4</i>, <i>JrMAPK5</i>, <i>JrMAPK6</i>, <i>JrMAPK7</i>, <i>JrMAPK9</i>, <i>JrMAPK11</i>, <i>JrMAPK12</i>, <i>JrMAPK13</i>, <i>JrMAPK17</i>, <i>JrMAPK19</i>, <i>JrMAPK20</i>, and <i>JrMAPK21</i> were upregulated at the transcriptional level in response to the drought stress treatment. The results of this study will help in further investigations of the evolutionary history and biological functions of the MAPK gene family in walnut.https://www.mdpi.com/2223-7747/12/3/586MAPK gene family<i>Juglans regia</i>genome-wide analysis<i>Colletotrichum gloeosporioides</i>drought stressexpression analysis |
| spellingShingle | Kaiyu Yang Jianghao Wu Xialei Wang Han Li Peng Jia Haoan Luan Xuemei Zhang Suping Guo Minsheng Yang Qinglong Dong Guohui Qi Genome-Wide Characterization of the Mitogen-Activated Protein Kinase Gene Family and Their Expression Patterns in Response to Drought and Colletotrichum Gloeosporioides in Walnut (<i>Juglans regia</i>) Plants MAPK gene family <i>Juglans regia</i> genome-wide analysis <i>Colletotrichum gloeosporioides</i> drought stress expression analysis |
| title | Genome-Wide Characterization of the Mitogen-Activated Protein Kinase Gene Family and Their Expression Patterns in Response to Drought and Colletotrichum Gloeosporioides in Walnut (<i>Juglans regia</i>) |
| title_full | Genome-Wide Characterization of the Mitogen-Activated Protein Kinase Gene Family and Their Expression Patterns in Response to Drought and Colletotrichum Gloeosporioides in Walnut (<i>Juglans regia</i>) |
| title_fullStr | Genome-Wide Characterization of the Mitogen-Activated Protein Kinase Gene Family and Their Expression Patterns in Response to Drought and Colletotrichum Gloeosporioides in Walnut (<i>Juglans regia</i>) |
| title_full_unstemmed | Genome-Wide Characterization of the Mitogen-Activated Protein Kinase Gene Family and Their Expression Patterns in Response to Drought and Colletotrichum Gloeosporioides in Walnut (<i>Juglans regia</i>) |
| title_short | Genome-Wide Characterization of the Mitogen-Activated Protein Kinase Gene Family and Their Expression Patterns in Response to Drought and Colletotrichum Gloeosporioides in Walnut (<i>Juglans regia</i>) |
| title_sort | genome wide characterization of the mitogen activated protein kinase gene family and their expression patterns in response to drought and colletotrichum gloeosporioides in walnut i juglans regia i |
| topic | MAPK gene family <i>Juglans regia</i> genome-wide analysis <i>Colletotrichum gloeosporioides</i> drought stress expression analysis |
| url | https://www.mdpi.com/2223-7747/12/3/586 |
| work_keys_str_mv | AT kaiyuyang genomewidecharacterizationofthemitogenactivatedproteinkinasegenefamilyandtheirexpressionpatternsinresponsetodroughtandcolletotrichumgloeosporioidesinwalnutijuglansregiai AT jianghaowu genomewidecharacterizationofthemitogenactivatedproteinkinasegenefamilyandtheirexpressionpatternsinresponsetodroughtandcolletotrichumgloeosporioidesinwalnutijuglansregiai AT xialeiwang genomewidecharacterizationofthemitogenactivatedproteinkinasegenefamilyandtheirexpressionpatternsinresponsetodroughtandcolletotrichumgloeosporioidesinwalnutijuglansregiai AT hanli genomewidecharacterizationofthemitogenactivatedproteinkinasegenefamilyandtheirexpressionpatternsinresponsetodroughtandcolletotrichumgloeosporioidesinwalnutijuglansregiai AT pengjia genomewidecharacterizationofthemitogenactivatedproteinkinasegenefamilyandtheirexpressionpatternsinresponsetodroughtandcolletotrichumgloeosporioidesinwalnutijuglansregiai AT haoanluan genomewidecharacterizationofthemitogenactivatedproteinkinasegenefamilyandtheirexpressionpatternsinresponsetodroughtandcolletotrichumgloeosporioidesinwalnutijuglansregiai AT xuemeizhang genomewidecharacterizationofthemitogenactivatedproteinkinasegenefamilyandtheirexpressionpatternsinresponsetodroughtandcolletotrichumgloeosporioidesinwalnutijuglansregiai AT supingguo genomewidecharacterizationofthemitogenactivatedproteinkinasegenefamilyandtheirexpressionpatternsinresponsetodroughtandcolletotrichumgloeosporioidesinwalnutijuglansregiai AT minshengyang genomewidecharacterizationofthemitogenactivatedproteinkinasegenefamilyandtheirexpressionpatternsinresponsetodroughtandcolletotrichumgloeosporioidesinwalnutijuglansregiai AT qinglongdong genomewidecharacterizationofthemitogenactivatedproteinkinasegenefamilyandtheirexpressionpatternsinresponsetodroughtandcolletotrichumgloeosporioidesinwalnutijuglansregiai AT guohuiqi genomewidecharacterizationofthemitogenactivatedproteinkinasegenefamilyandtheirexpressionpatternsinresponsetodroughtandcolletotrichumgloeosporioidesinwalnutijuglansregiai |