Control of meiotic entry by dual inhibition of a key mitotic transcription factor
The mitosis to meiosis transition requires dynamic changes in gene expression, but whether and how the mitotic transcriptional machinery is regulated during this transition is unknown. In budding yeast, SBF and MBF transcription factors initiate the mitotic gene expression program. Here, we report t...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2024-02-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/90425 |
| _version_ | 1797262420721270784 |
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| author | Amanda J Su Siri C Yendluri Elçin Ünal |
| author_facet | Amanda J Su Siri C Yendluri Elçin Ünal |
| author_sort | Amanda J Su |
| collection | DOAJ |
| description | The mitosis to meiosis transition requires dynamic changes in gene expression, but whether and how the mitotic transcriptional machinery is regulated during this transition is unknown. In budding yeast, SBF and MBF transcription factors initiate the mitotic gene expression program. Here, we report two mechanisms that work together to restrict SBF activity during meiotic entry: repression of the SBF-specific Swi4 subunit through LUTI-based regulation and inhibition of SBF by Whi5, a functional homolog of the Rb tumor suppressor. We find that untimely SBF activation causes downregulation of early meiotic genes and delays meiotic entry. These defects are largely driven by the SBF-target G1 cyclins, which block the interaction between the central meiotic regulator Ime1 and its cofactor Ume6. Our study provides insight into the role of SWI4LUTI in establishing the meiotic transcriptional program and demonstrates how the LUTI-based regulation is integrated into a larger regulatory network to ensure timely SBF activity. |
| first_indexed | 2024-03-07T21:20:35Z |
| format | Article |
| id | doaj.art-0ded6447d09f46b79b0458e565096454 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-24T23:56:50Z |
| publishDate | 2024-02-01 |
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| spelling | doaj.art-0ded6447d09f46b79b0458e5650964542024-03-14T11:03:23ZengeLife Sciences Publications LtdeLife2050-084X2024-02-011210.7554/eLife.90425Control of meiotic entry by dual inhibition of a key mitotic transcription factorAmanda J Su0https://orcid.org/0009-0002-4203-1713Siri C Yendluri1Elçin Ünal2https://orcid.org/0000-0002-6768-609XDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United StatesDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United StatesDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United StatesThe mitosis to meiosis transition requires dynamic changes in gene expression, but whether and how the mitotic transcriptional machinery is regulated during this transition is unknown. In budding yeast, SBF and MBF transcription factors initiate the mitotic gene expression program. Here, we report two mechanisms that work together to restrict SBF activity during meiotic entry: repression of the SBF-specific Swi4 subunit through LUTI-based regulation and inhibition of SBF by Whi5, a functional homolog of the Rb tumor suppressor. We find that untimely SBF activation causes downregulation of early meiotic genes and delays meiotic entry. These defects are largely driven by the SBF-target G1 cyclins, which block the interaction between the central meiotic regulator Ime1 and its cofactor Ume6. Our study provides insight into the role of SWI4LUTI in establishing the meiotic transcriptional program and demonstrates how the LUTI-based regulation is integrated into a larger regulatory network to ensure timely SBF activity.https://elifesciences.org/articles/90425meiosismitosistranscription factorcell cycleLUTIuORF |
| spellingShingle | Amanda J Su Siri C Yendluri Elçin Ünal Control of meiotic entry by dual inhibition of a key mitotic transcription factor eLife meiosis mitosis transcription factor cell cycle LUTI uORF |
| title | Control of meiotic entry by dual inhibition of a key mitotic transcription factor |
| title_full | Control of meiotic entry by dual inhibition of a key mitotic transcription factor |
| title_fullStr | Control of meiotic entry by dual inhibition of a key mitotic transcription factor |
| title_full_unstemmed | Control of meiotic entry by dual inhibition of a key mitotic transcription factor |
| title_short | Control of meiotic entry by dual inhibition of a key mitotic transcription factor |
| title_sort | control of meiotic entry by dual inhibition of a key mitotic transcription factor |
| topic | meiosis mitosis transcription factor cell cycle LUTI uORF |
| url | https://elifesciences.org/articles/90425 |
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