Resveratrol promotes secretion of wound healing related growth factors of mesenchymal stem cells originated from adult and fetal tissues
AbstractNon-healing wounds have demonstrated aberrant regulation of several growth factors, thus using exogenous growth factors and cytokines in the clinical setting may improve the outcomes of non-healing wounds. Mesenchymal stem cells (MSCs) are the source of growth factors that show beneficial ef...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2020.1817057 |
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| author | Cita R. S. Prakoeswa Yuyun Rindiastuti Yohanes Widodo Wirohadidjojo Evelyn Komaratih Nurwasis Aristika Dinaryati Ni Made Inten Lestari Fedik A. Rantam |
| author_facet | Cita R. S. Prakoeswa Yuyun Rindiastuti Yohanes Widodo Wirohadidjojo Evelyn Komaratih Nurwasis Aristika Dinaryati Ni Made Inten Lestari Fedik A. Rantam |
| author_sort | Cita R. S. Prakoeswa |
| collection | DOAJ |
| description | AbstractNon-healing wounds have demonstrated aberrant regulation of several growth factors, thus using exogenous growth factors and cytokines in the clinical setting may improve the outcomes of non-healing wounds. Mesenchymal stem cells (MSCs) are the source of growth factors that show beneficial effect in promoting impaired wound healing. Certain culture condition should be developed to stimulate growth factor secretion from stem cell. Resveratrol, a small molecule found to increase MSCs therapeutic effectiveness. This study aims to investigate the effect of RV on secretion of wound healing related growth factors. We isolated and characterised MSCs from wharton’s jelly (WJ), amniotic membrane (AM), and adipose tissue. We treated MSCs with serum deprived medium (SDM) supplemented with RV at 0.1 µM, 0.5 µM, 0.8 µM concentration. Our study revealed that RV at 0.1 µM was more effective to increase cell proliferation rate. Resveratrol at 0.1 µM promoted EGF, HGF, PDGF, and TGF-β1 secretion from MSCs. AD-MSCs showed the greatest response to RV stimulation in the term of cell proliferation and growth factors secretion. As conclusion, RV can facilitate cell proliferation and wound healing related growth factors secretion at dosage dependent manner. |
| first_indexed | 2024-03-13T01:40:22Z |
| format | Article |
| id | doaj.art-0df2434d977b46ef9e75dcb402381240 |
| institution | Directory Open Access Journal |
| issn | 2169-1401 2169-141X |
| language | English |
| last_indexed | 2024-03-13T01:40:22Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj.art-0df2434d977b46ef9e75dcb4023812402023-07-03T14:04:57ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2020-01-014811159116610.1080/21691401.2020.1817057Resveratrol promotes secretion of wound healing related growth factors of mesenchymal stem cells originated from adult and fetal tissuesCita R. S. Prakoeswa0Yuyun Rindiastuti1Yohanes Widodo Wirohadidjojo2Evelyn Komaratih3Nurwasis4Aristika Dinaryati5Ni Made Inten Lestari6Fedik A. Rantam7Department of Dermatovenerology, Faculty of Medicine Universitas Airlangga/Dr Soetomo, General Academic Hospital, Surabaya, IndonesiaDepartment of Ophthalmology, Faculty of Medicine Universitas Airlangga/Dr Soetomo, General Academic Hospital, Surabaya, IndonesiaDepartment of Dermatovenerology, Faculty of Medicine Universitas Gadjah Mada/Dr Sardjito, General Academic Hospital, Yogyakarta, IndonesiaDepartment of Ophthalmology, Faculty of Medicine Universitas Airlangga/Dr Soetomo, General Academic Hospital, Surabaya, IndonesiaDepartment of Ophthalmology, Faculty of Medicine Universitas Airlangga/Dr Soetomo, General Academic Hospital, Surabaya, IndonesiaStem Cell Research and Development Center, Universitas Airlangga, Surabaya, IndonesiaDepartment of Dermatovenerology, Faculty of Medicine Universitas Airlangga/Dr Soetomo, General Academic Hospital, Surabaya, IndonesiaStem Cell Research and Development Center, Universitas Airlangga, Surabaya, IndonesiaAbstractNon-healing wounds have demonstrated aberrant regulation of several growth factors, thus using exogenous growth factors and cytokines in the clinical setting may improve the outcomes of non-healing wounds. Mesenchymal stem cells (MSCs) are the source of growth factors that show beneficial effect in promoting impaired wound healing. Certain culture condition should be developed to stimulate growth factor secretion from stem cell. Resveratrol, a small molecule found to increase MSCs therapeutic effectiveness. This study aims to investigate the effect of RV on secretion of wound healing related growth factors. We isolated and characterised MSCs from wharton’s jelly (WJ), amniotic membrane (AM), and adipose tissue. We treated MSCs with serum deprived medium (SDM) supplemented with RV at 0.1 µM, 0.5 µM, 0.8 µM concentration. Our study revealed that RV at 0.1 µM was more effective to increase cell proliferation rate. Resveratrol at 0.1 µM promoted EGF, HGF, PDGF, and TGF-β1 secretion from MSCs. AD-MSCs showed the greatest response to RV stimulation in the term of cell proliferation and growth factors secretion. As conclusion, RV can facilitate cell proliferation and wound healing related growth factors secretion at dosage dependent manner.https://www.tandfonline.com/doi/10.1080/21691401.2020.1817057Wound healingstem cellresveratrolsecretome |
| spellingShingle | Cita R. S. Prakoeswa Yuyun Rindiastuti Yohanes Widodo Wirohadidjojo Evelyn Komaratih Nurwasis Aristika Dinaryati Ni Made Inten Lestari Fedik A. Rantam Resveratrol promotes secretion of wound healing related growth factors of mesenchymal stem cells originated from adult and fetal tissues Artificial Cells, Nanomedicine, and Biotechnology Wound healing stem cell resveratrol secretome |
| title | Resveratrol promotes secretion of wound healing related growth factors of mesenchymal stem cells originated from adult and fetal tissues |
| title_full | Resveratrol promotes secretion of wound healing related growth factors of mesenchymal stem cells originated from adult and fetal tissues |
| title_fullStr | Resveratrol promotes secretion of wound healing related growth factors of mesenchymal stem cells originated from adult and fetal tissues |
| title_full_unstemmed | Resveratrol promotes secretion of wound healing related growth factors of mesenchymal stem cells originated from adult and fetal tissues |
| title_short | Resveratrol promotes secretion of wound healing related growth factors of mesenchymal stem cells originated from adult and fetal tissues |
| title_sort | resveratrol promotes secretion of wound healing related growth factors of mesenchymal stem cells originated from adult and fetal tissues |
| topic | Wound healing stem cell resveratrol secretome |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2020.1817057 |
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