Visualizing Cellular Dynamics and Protein Localization in 3D Collagen
Summary: Immune cells migrate and communicate through cell-to-cell interactions and cytokines to coordinate the specificity and timing of the immune response. While studying these events in cell culture are standard procedure, spatiotemporal dynamics of cell-to-cell interactions within three-dimensi...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166720301908 |
| _version_ | 1819077411257450496 |
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| author | Wan Hon Koh Romaniya Zayats Paul Lopez Thomas T. Murooka |
| author_facet | Wan Hon Koh Romaniya Zayats Paul Lopez Thomas T. Murooka |
| author_sort | Wan Hon Koh |
| collection | DOAJ |
| description | Summary: Immune cells migrate and communicate through cell-to-cell interactions and cytokines to coordinate the specificity and timing of the immune response. While studying these events in cell culture are standard procedure, spatiotemporal dynamics of cell-to-cell interactions within three-dimensional (3D) environments are critical in generating appropriate effector functions. Here, we present a detailed protocol to study cells within an all-in-one 3D collagen matrix that is amenable to live-cell microscopy and immunohistochemistry. This approach facilitates analyses of dynamic cellular events in 3D settings.For complete details on the use and execution of this protocol, please refer to Koh et al. (2020). |
| first_indexed | 2024-12-21T18:56:46Z |
| format | Article |
| id | doaj.art-0df31cb2a2a44fd7951e9409034edb37 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-21T18:56:46Z |
| publishDate | 2020-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-0df31cb2a2a44fd7951e9409034edb372022-12-21T18:53:35ZengElsevierSTAR Protocols2666-16672020-12-0113100203Visualizing Cellular Dynamics and Protein Localization in 3D CollagenWan Hon Koh0Romaniya Zayats1Paul Lopez2Thomas T. Murooka3University of Manitoba, Rady Faculty of Health Sciences, Department of Immunology, Winnipeg, CanadaUniversity of Manitoba, Rady Faculty of Health Sciences, Department of Immunology, Winnipeg, CanadaUniversity of Manitoba, Rady Faculty of Health Sciences, Department of Immunology, Winnipeg, CanadaUniversity of Manitoba, Rady Faculty of Health Sciences, Department of Immunology, Winnipeg, Canada; University of Manitoba, Rady Faculty of Health Science, Department of Medical Microbiology and Infectious Diseases, Winnipeg, Canada; Corresponding authorSummary: Immune cells migrate and communicate through cell-to-cell interactions and cytokines to coordinate the specificity and timing of the immune response. While studying these events in cell culture are standard procedure, spatiotemporal dynamics of cell-to-cell interactions within three-dimensional (3D) environments are critical in generating appropriate effector functions. Here, we present a detailed protocol to study cells within an all-in-one 3D collagen matrix that is amenable to live-cell microscopy and immunohistochemistry. This approach facilitates analyses of dynamic cellular events in 3D settings.For complete details on the use and execution of this protocol, please refer to Koh et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166720301908Cell BiologyCell-based AssaysImmunologyMicroscopy |
| spellingShingle | Wan Hon Koh Romaniya Zayats Paul Lopez Thomas T. Murooka Visualizing Cellular Dynamics and Protein Localization in 3D Collagen STAR Protocols Cell Biology Cell-based Assays Immunology Microscopy |
| title | Visualizing Cellular Dynamics and Protein Localization in 3D Collagen |
| title_full | Visualizing Cellular Dynamics and Protein Localization in 3D Collagen |
| title_fullStr | Visualizing Cellular Dynamics and Protein Localization in 3D Collagen |
| title_full_unstemmed | Visualizing Cellular Dynamics and Protein Localization in 3D Collagen |
| title_short | Visualizing Cellular Dynamics and Protein Localization in 3D Collagen |
| title_sort | visualizing cellular dynamics and protein localization in 3d collagen |
| topic | Cell Biology Cell-based Assays Immunology Microscopy |
| url | http://www.sciencedirect.com/science/article/pii/S2666166720301908 |
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