Hsa_circ_0002111/miR-557/DUSP14 axis mediates euthyrox-resistance in papillary thyroid cancer
As one of the most commonly used chemotherapeutic drug for papillary thyroid cancer (PTC), euthyrox affects the therapeutic outcome due to the resistance of euthyrox. Hsa_circ_0002111 is highly expressed in euthyrox-resistant PTC patients, and this study intends to explore its role in euthyrox drug...
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Innovation Publishing House Pte. Ltd.
2022-04-01
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| Series: | STEMedicine |
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| Online Access: | https://stemedicine.org/index.php/stem/article/view/127 |
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| author | Jing Zhou Jing Liu Weiyuan Ma Pengxin Zhao |
| author_facet | Jing Zhou Jing Liu Weiyuan Ma Pengxin Zhao |
| author_sort | Jing Zhou |
| collection | DOAJ |
| description |
As one of the most commonly used chemotherapeutic drug for papillary thyroid cancer (PTC), euthyrox affects the therapeutic outcome due to the resistance of euthyrox. Hsa_circ_0002111 is highly expressed in euthyrox-resistant PTC patients, and this study intends to explore its role in euthyrox drug resistance. PTC patient samples were used to screen for Circ_0002111 expression. TPC-1 and K1 PTC cell lines and their corresponding euthyrox-resistant cell lines (TPC-1/euthyrox and K1/euthyrox), and a benign human thyroid follicular cell line (Nthy-ori 3-1) were used in in vitro experiments. Circ_0002111 was knocked down in euthyrox-resistant cell lines, and cell viability and colony formation were detected. Caspase-3 activity assay and nucleosomal fragmentation assay were used for the detection of apoptosis. Luciferase reporter assay and biotin-labeled RNA pulldown assay were used to analyze interactions between Circ_0002111 and miR-557, or miR-557 and DUSP14. The upregulation of Circ_0002111 was found in PTC patient samples and associated with euthyrox-resistance in poor prognosis of PTC patients. Experiments in cell lines showed that Circ_0002111 regulates euthyrox-resistance in PTC cells. Mechanistic studies showed that Circ_0002111 promoted DUSP14 expression through miR-557, and euthyrox-resistance in PTC cells depended on the regulation via miR-557/DUSP14 signaling pathways. In conclusion, Hsa_circ_0002111 promotes euthyrox-resistance of PTC cells by adsorption miR-557 upregulation, suggesting Circ_0002111 might be a potential diagnostic marker and therapeutic target for euthyrox-resistant PTC patients.
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| first_indexed | 2024-04-13T10:18:08Z |
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| language | English |
| last_indexed | 2024-04-13T10:18:08Z |
| publishDate | 2022-04-01 |
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| spelling | doaj.art-0df4a530cdf1471b865dc36647f1a7e42022-12-22T02:50:35ZengInnovation Publishing House Pte. Ltd.STEMedicine2705-11882022-04-013210.37175/stemedicine.v3i2.127Hsa_circ_0002111/miR-557/DUSP14 axis mediates euthyrox-resistance in papillary thyroid cancerJing Zhou0Jing Liu1Weiyuan Ma2Pengxin Zhao3The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, ChinaThe Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, ChinaThe Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, ChinaThe Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, China As one of the most commonly used chemotherapeutic drug for papillary thyroid cancer (PTC), euthyrox affects the therapeutic outcome due to the resistance of euthyrox. Hsa_circ_0002111 is highly expressed in euthyrox-resistant PTC patients, and this study intends to explore its role in euthyrox drug resistance. PTC patient samples were used to screen for Circ_0002111 expression. TPC-1 and K1 PTC cell lines and their corresponding euthyrox-resistant cell lines (TPC-1/euthyrox and K1/euthyrox), and a benign human thyroid follicular cell line (Nthy-ori 3-1) were used in in vitro experiments. Circ_0002111 was knocked down in euthyrox-resistant cell lines, and cell viability and colony formation were detected. Caspase-3 activity assay and nucleosomal fragmentation assay were used for the detection of apoptosis. Luciferase reporter assay and biotin-labeled RNA pulldown assay were used to analyze interactions between Circ_0002111 and miR-557, or miR-557 and DUSP14. The upregulation of Circ_0002111 was found in PTC patient samples and associated with euthyrox-resistance in poor prognosis of PTC patients. Experiments in cell lines showed that Circ_0002111 regulates euthyrox-resistance in PTC cells. Mechanistic studies showed that Circ_0002111 promoted DUSP14 expression through miR-557, and euthyrox-resistance in PTC cells depended on the regulation via miR-557/DUSP14 signaling pathways. In conclusion, Hsa_circ_0002111 promotes euthyrox-resistance of PTC cells by adsorption miR-557 upregulation, suggesting Circ_0002111 might be a potential diagnostic marker and therapeutic target for euthyrox-resistant PTC patients. https://stemedicine.org/index.php/stem/article/view/127miR-557Circ_0002111Euthyrox resistantDUSP14Umael |
| spellingShingle | Jing Zhou Jing Liu Weiyuan Ma Pengxin Zhao Hsa_circ_0002111/miR-557/DUSP14 axis mediates euthyrox-resistance in papillary thyroid cancer STEMedicine miR-557 Circ_0002111 Euthyrox resistant DUSP14 Umael |
| title | Hsa_circ_0002111/miR-557/DUSP14 axis mediates euthyrox-resistance in papillary thyroid cancer |
| title_full | Hsa_circ_0002111/miR-557/DUSP14 axis mediates euthyrox-resistance in papillary thyroid cancer |
| title_fullStr | Hsa_circ_0002111/miR-557/DUSP14 axis mediates euthyrox-resistance in papillary thyroid cancer |
| title_full_unstemmed | Hsa_circ_0002111/miR-557/DUSP14 axis mediates euthyrox-resistance in papillary thyroid cancer |
| title_short | Hsa_circ_0002111/miR-557/DUSP14 axis mediates euthyrox-resistance in papillary thyroid cancer |
| title_sort | hsa circ 0002111 mir 557 dusp14 axis mediates euthyrox resistance in papillary thyroid cancer |
| topic | miR-557 Circ_0002111 Euthyrox resistant DUSP14 Umael |
| url | https://stemedicine.org/index.php/stem/article/view/127 |
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