Regulation of eukaryotic initiation factor 4AII by MyoD during murine myogenic cell differentiation.
Gene expression during muscle cell differentiation is tightly regulated at multiple levels, including translation initiation. The PI3K/mTOR signalling pathway exerts control over protein synthesis by regulating assembly of eukaryotic initiation factor (eIF) 4F, a heterotrimeric complex that stimulat...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3900710?pdf=render |
| _version_ | 1818040276360364032 |
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| author | Gabriela Galicia-Vázquez Sergio Di Marco Xian J Lian Jennifer F Ma Imed E Gallouzi Jerry Pelletier |
| author_facet | Gabriela Galicia-Vázquez Sergio Di Marco Xian J Lian Jennifer F Ma Imed E Gallouzi Jerry Pelletier |
| author_sort | Gabriela Galicia-Vázquez |
| collection | DOAJ |
| description | Gene expression during muscle cell differentiation is tightly regulated at multiple levels, including translation initiation. The PI3K/mTOR signalling pathway exerts control over protein synthesis by regulating assembly of eukaryotic initiation factor (eIF) 4F, a heterotrimeric complex that stimulates recruitment of ribosomes to mRNA templates. One of the subunits of eIF4F, eIF4A, supplies essential helicase function during this phase of translation. The presence of two cellular eIF4A isoforms, eIF4AI and eIF4AII, has long thought to impart equivalent functions to eIF4F. However, recent experiments have alluded to distinct activities between them. Herein, we characterize distinct regulatory mechanisms between the eIF4A isoforms during muscle cell differentiation. We find that eIF4AI levels decrease during differentiation whereas eIF4AII levels increase during myofiber formation in a MyoD-dependent manner. This study characterizes a previously undefined mechanism for eIF4AII regulation in differentiation and highlights functional differences between eIF4AI and eIF4AII. Finally, RNAi-mediated alterations in eIF4AI and eIF4AII levels indicate that the myogenic process can tolerate short term reductions in eIF4AI or eIF4AII levels, but not both. |
| first_indexed | 2024-12-10T08:11:57Z |
| format | Article |
| id | doaj.art-0df4c202c8244fadac522a12b61024a8 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T08:11:57Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-0df4c202c8244fadac522a12b61024a82022-12-22T01:56:33ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0191e8723710.1371/journal.pone.0087237Regulation of eukaryotic initiation factor 4AII by MyoD during murine myogenic cell differentiation.Gabriela Galicia-VázquezSergio Di MarcoXian J LianJennifer F MaImed E GallouziJerry PelletierGene expression during muscle cell differentiation is tightly regulated at multiple levels, including translation initiation. The PI3K/mTOR signalling pathway exerts control over protein synthesis by regulating assembly of eukaryotic initiation factor (eIF) 4F, a heterotrimeric complex that stimulates recruitment of ribosomes to mRNA templates. One of the subunits of eIF4F, eIF4A, supplies essential helicase function during this phase of translation. The presence of two cellular eIF4A isoforms, eIF4AI and eIF4AII, has long thought to impart equivalent functions to eIF4F. However, recent experiments have alluded to distinct activities between them. Herein, we characterize distinct regulatory mechanisms between the eIF4A isoforms during muscle cell differentiation. We find that eIF4AI levels decrease during differentiation whereas eIF4AII levels increase during myofiber formation in a MyoD-dependent manner. This study characterizes a previously undefined mechanism for eIF4AII regulation in differentiation and highlights functional differences between eIF4AI and eIF4AII. Finally, RNAi-mediated alterations in eIF4AI and eIF4AII levels indicate that the myogenic process can tolerate short term reductions in eIF4AI or eIF4AII levels, but not both.http://europepmc.org/articles/PMC3900710?pdf=render |
| spellingShingle | Gabriela Galicia-Vázquez Sergio Di Marco Xian J Lian Jennifer F Ma Imed E Gallouzi Jerry Pelletier Regulation of eukaryotic initiation factor 4AII by MyoD during murine myogenic cell differentiation. PLoS ONE |
| title | Regulation of eukaryotic initiation factor 4AII by MyoD during murine myogenic cell differentiation. |
| title_full | Regulation of eukaryotic initiation factor 4AII by MyoD during murine myogenic cell differentiation. |
| title_fullStr | Regulation of eukaryotic initiation factor 4AII by MyoD during murine myogenic cell differentiation. |
| title_full_unstemmed | Regulation of eukaryotic initiation factor 4AII by MyoD during murine myogenic cell differentiation. |
| title_short | Regulation of eukaryotic initiation factor 4AII by MyoD during murine myogenic cell differentiation. |
| title_sort | regulation of eukaryotic initiation factor 4aii by myod during murine myogenic cell differentiation |
| url | http://europepmc.org/articles/PMC3900710?pdf=render |
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