Larvicidal effect of an N-isobutyl-(2E,4E,8Z,10E/Z)-dodecatetraenamides-rich extract of Salmea scandens on fall armyworm
Aim of study: To determine if secondary metabolites present in an ethanolic extract of Salmea scandens could be considered as a viable alternative for the control of fall armyworm (Spodoptera frugiperda larvae), as this is the most important maize pest in terms of economic losses to agriculture wor...
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| Format: | Article |
| Language: | English |
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Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
2023-10-01
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| Series: | Spanish Journal of Agricultural Research |
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| Online Access: | https://sjar.revistas.csic.es/index.php/sjar/article/view/20275 |
| _version_ | 1797627645538598912 |
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| author | Mary Carmen PACHECO-ESTEVA Delia SOTO-CASTRO Jaime RUIZ-VEGA María Eugenia OCHOA Pedro MONTES-GARCÍA |
| author_facet | Mary Carmen PACHECO-ESTEVA Delia SOTO-CASTRO Jaime RUIZ-VEGA María Eugenia OCHOA Pedro MONTES-GARCÍA |
| author_sort | Mary Carmen PACHECO-ESTEVA |
| collection | DOAJ |
| description |
Aim of study: To determine if secondary metabolites present in an ethanolic extract of Salmea scandens could be considered as a viable alternative for the control of fall armyworm (Spodoptera frugiperda larvae), as this is the most important maize pest in terms of economic losses to agriculture worldwide.
Area of study: S. scandens shrubs were collected in San Rafael Toltepec, Oaxaca, Mexico. The laboratory assays were conducted at CIIDIR Oaxaca, and preliminary field assay was carried out in Zaachila Oaxaca.
Material and methods: N-isobutyl-(2E,4E,8Z,10E/Z)-dodecatetraenamides-rich extract of S. scandens, corroborated by nuclear magnetic resonance (1H NMR and 13C NMR) and Fourier transform infrared (FTIR), was obtained from S. scandens by 10-day maceration in ethanol. The effect of the extract on the mortality of S. frugiperda larvae was investigated in the laboratory (in vitro) by topical application, and in the field (in situ), testing both topical and spraying applications.
Main results: The 1H NMR, 13C NMR and FTIR spectra evidenced the obtention of the alkylamides-rich ethanol extract of S. scandens. Mortality of S. frugiperda in vitro increased with dose and monitoring time, reaching up to 80%. Under field conditions 63% of mortality was recorded at a dose of 0.30 mg µL-1 per larva by topical application, and 49% by spray application at a dose of 0.15 mg µL-1 per larva.
Research highlights: The ethanolic extract of S. scandens can be considered a viable alternative for controlling fall armyworm S. frugiperda.
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| first_indexed | 2024-03-11T10:27:17Z |
| format | Article |
| id | doaj.art-0df5106b951844c2b721c2b79ffa1ecd |
| institution | Directory Open Access Journal |
| issn | 1695-971X 2171-9292 |
| language | English |
| last_indexed | 2024-03-11T10:27:17Z |
| publishDate | 2023-10-01 |
| publisher | Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria |
| record_format | Article |
| series | Spanish Journal of Agricultural Research |
| spelling | doaj.art-0df5106b951844c2b721c2b79ffa1ecd2023-11-15T08:51:44ZengInstituto Nacional de Investigación y Tecnología Agraria y AlimentariaSpanish Journal of Agricultural Research1695-971X2171-92922023-10-0121410.5424/sjar/2023214-20275Larvicidal effect of an N-isobutyl-(2E,4E,8Z,10E/Z)-dodecatetraenamides-rich extract of Salmea scandens on fall armywormMary Carmen PACHECO-ESTEVA0Delia SOTO-CASTRO1Jaime RUIZ-VEGA2María Eugenia OCHOA3Pedro MONTES-GARCÍA4Instituto Politécnico Nacional, CIIDIR Unidad Oaxaca, Hornos 1003, Santa Cruz Xoxocotlán, Oaxaca, 771230 MexicoCONACyT-Instituto Politécnico Nacional, CIIDIR Unidad Oaxaca, Hornos 1003, Santa Cruz Xoxocotlán, Oaxaca 771230 MexicoInstituto Politécnico Nacional, CIIDIR Unidad Oaxaca, Hornos 1003, Santa Cruz Xoxocotlán, Oaxaca, 771230 MexicoDepartamento de Química, Centro de Investigación y de Estudios Avanzados del IPN, Mexico D.F, Apdo. Postal 14-740, 07000 Ciudad de México, MexicoInstituto Politécnico Nacional, CIIDIR Unidad Oaxaca, Hornos 1003, Santa Cruz Xoxocotlán, Oaxaca, 771230 Mexico Aim of study: To determine if secondary metabolites present in an ethanolic extract of Salmea scandens could be considered as a viable alternative for the control of fall armyworm (Spodoptera frugiperda larvae), as this is the most important maize pest in terms of economic losses to agriculture worldwide. Area of study: S. scandens shrubs were collected in San Rafael Toltepec, Oaxaca, Mexico. The laboratory assays were conducted at CIIDIR Oaxaca, and preliminary field assay was carried out in Zaachila Oaxaca. Material and methods: N-isobutyl-(2E,4E,8Z,10E/Z)-dodecatetraenamides-rich extract of S. scandens, corroborated by nuclear magnetic resonance (1H NMR and 13C NMR) and Fourier transform infrared (FTIR), was obtained from S. scandens by 10-day maceration in ethanol. The effect of the extract on the mortality of S. frugiperda larvae was investigated in the laboratory (in vitro) by topical application, and in the field (in situ), testing both topical and spraying applications. Main results: The 1H NMR, 13C NMR and FTIR spectra evidenced the obtention of the alkylamides-rich ethanol extract of S. scandens. Mortality of S. frugiperda in vitro increased with dose and monitoring time, reaching up to 80%. Under field conditions 63% of mortality was recorded at a dose of 0.30 mg µL-1 per larva by topical application, and 49% by spray application at a dose of 0.15 mg µL-1 per larva. Research highlights: The ethanolic extract of S. scandens can be considered a viable alternative for controlling fall armyworm S. frugiperda. https://sjar.revistas.csic.es/index.php/sjar/article/view/20275bio-controllersmacerationN-isobutyl-(2E,4E,8Z,10E/Z)-dodecatetraenamidespest controlplant extract |
| spellingShingle | Mary Carmen PACHECO-ESTEVA Delia SOTO-CASTRO Jaime RUIZ-VEGA María Eugenia OCHOA Pedro MONTES-GARCÍA Larvicidal effect of an N-isobutyl-(2E,4E,8Z,10E/Z)-dodecatetraenamides-rich extract of Salmea scandens on fall armyworm Spanish Journal of Agricultural Research bio-controllers maceration N-isobutyl-(2E,4E,8Z,10E/Z)-dodecatetraenamides pest control plant extract |
| title | Larvicidal effect of an N-isobutyl-(2E,4E,8Z,10E/Z)-dodecatetraenamides-rich extract of Salmea scandens on fall armyworm |
| title_full | Larvicidal effect of an N-isobutyl-(2E,4E,8Z,10E/Z)-dodecatetraenamides-rich extract of Salmea scandens on fall armyworm |
| title_fullStr | Larvicidal effect of an N-isobutyl-(2E,4E,8Z,10E/Z)-dodecatetraenamides-rich extract of Salmea scandens on fall armyworm |
| title_full_unstemmed | Larvicidal effect of an N-isobutyl-(2E,4E,8Z,10E/Z)-dodecatetraenamides-rich extract of Salmea scandens on fall armyworm |
| title_short | Larvicidal effect of an N-isobutyl-(2E,4E,8Z,10E/Z)-dodecatetraenamides-rich extract of Salmea scandens on fall armyworm |
| title_sort | larvicidal effect of an n isobutyl 2e 4e 8z 10e z dodecatetraenamides rich extract of salmea scandens on fall armyworm |
| topic | bio-controllers maceration N-isobutyl-(2E,4E,8Z,10E/Z)-dodecatetraenamides pest control plant extract |
| url | https://sjar.revistas.csic.es/index.php/sjar/article/view/20275 |
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