LncRNA SNHG7 enhances the drug resistance of human acute myeloid leukemia cells through inhibiting miR-186
Objective To investigate the effect of long non-coding RNA (lncRNA) SNHG7 on cell drug resistance through inhibiting microRNA-186(miR-186). Methods Peripheral blood was taken from newly diagnosed AML patients, recurrent/refractory AML patients and healthy controls. AML adriamycin sensitive cells (...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2021-02-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a200521.pdf |
| _version_ | 1797365764924112896 |
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| author | ZHU Juan, ZHOU Ying, LI Ying-jia |
| author_facet | ZHU Juan, ZHOU Ying, LI Ying-jia |
| author_sort | ZHU Juan, ZHOU Ying, LI Ying-jia |
| collection | DOAJ |
| description | Objective To investigate the effect of long non-coding RNA (lncRNA) SNHG7 on cell drug resistance through inhibiting microRNA-186(miR-186). Methods Peripheral blood was taken from newly diagnosed AML patients, recurrent/refractory AML patients and healthy controls. AML adriamycin sensitive cells (HL60) and AML adriamycin resistant cells (HL60/ADM) were used to detect the expression of SNHG7 and miR-186 in peripheral blood and cell samples by fluorescence quantitative PCR. The sensitivity of HL60 and HL60/ADM cells to adriamycin was determined by CCK8 assay and the 50% inhibitory concentration(IC50) was calculated. Sh-SNHG7 or miR-186 mimic or miR-186 inhibitor was transfected into HL60/ADM cells. The expres- sion of SNHG7 and miR-186 was detected by fluorescence quantitative PCR, the dose response and IC50 of HL60/ADM to adriamycin were detected by CCK8. Double luciferase reporter gene assay was used to detect the targeted binding of SNHG7 to miR-186. Results The expression of SNHG7 in the serum of newly diagnosed AML and recurrent/refractory AML patients was significantly higher than that of normal controls, and the trend of miR-186 was contrary to that of SNHG7 (P＜0.01). Compared with HL60 cells, HL60/ADM cells were treated with adriamycin at different concentrations, and the IC50 value of adriamycin on HL60/ADM (3.36±0.65) cells was significantly higher than that of HL60 cells (0.43±0.16) (P＜0.001). SNHG7 expression was significantly higher(P＜0.05) in HL60/ADM cells and miR-186 was significantly lower (P＜0.05). Transfection with sh-SNHG7 or miR-186 mimic in HL60/ADM cells increased the sensitivity to adriamycin and decreased the IC50 value from 3.17±0.61 to 2.30±0.31 and 3.22±0.62 to 2.16±0.33, respectively (P＜0.05). Dual-luciferase reporter assay confirmed that SNHG7 could bind miR-186. Conclusions SNHG7 enhances drug resistance of AML cells during chemotherapy through inhibition of miR-186. |
| first_indexed | 2024-03-08T16:54:34Z |
| format | Article |
| id | doaj.art-0df52a42b9674fc0b8b2e4f5195ebf13 |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T16:54:34Z |
| publishDate | 2021-02-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-0df52a42b9674fc0b8b2e4f5195ebf132024-01-05T03:04:29ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252021-02-01412171177LncRNA SNHG7 enhances the drug resistance of human acute myeloid leukemia cells through inhibiting miR-186ZHU Juan, ZHOU Ying, LI Ying-jia01. Department of Laboratory Medicine, the Third Xiangya Hospital of Central South University, Changsha 410013; ;2. Department of Clinical Laboratory,Changsha Central Hospital,Changsha 410004, ChinaObjective To investigate the effect of long non-coding RNA (lncRNA) SNHG7 on cell drug resistance through inhibiting microRNA-186(miR-186). Methods Peripheral blood was taken from newly diagnosed AML patients, recurrent/refractory AML patients and healthy controls. AML adriamycin sensitive cells (HL60) and AML adriamycin resistant cells (HL60/ADM) were used to detect the expression of SNHG7 and miR-186 in peripheral blood and cell samples by fluorescence quantitative PCR. The sensitivity of HL60 and HL60/ADM cells to adriamycin was determined by CCK8 assay and the 50% inhibitory concentration(IC50) was calculated. Sh-SNHG7 or miR-186 mimic or miR-186 inhibitor was transfected into HL60/ADM cells. The expres- sion of SNHG7 and miR-186 was detected by fluorescence quantitative PCR, the dose response and IC50 of HL60/ADM to adriamycin were detected by CCK8. Double luciferase reporter gene assay was used to detect the targeted binding of SNHG7 to miR-186. Results The expression of SNHG7 in the serum of newly diagnosed AML and recurrent/refractory AML patients was significantly higher than that of normal controls, and the trend of miR-186 was contrary to that of SNHG7 (P＜0.01). Compared with HL60 cells, HL60/ADM cells were treated with adriamycin at different concentrations, and the IC50 value of adriamycin on HL60/ADM (3.36±0.65) cells was significantly higher than that of HL60 cells (0.43±0.16) (P＜0.001). SNHG7 expression was significantly higher(P＜0.05) in HL60/ADM cells and miR-186 was significantly lower (P＜0.05). Transfection with sh-SNHG7 or miR-186 mimic in HL60/ADM cells increased the sensitivity to adriamycin and decreased the IC50 value from 3.17±0.61 to 2.30±0.31 and 3.22±0.62 to 2.16±0.33, respectively (P＜0.05). Dual-luciferase reporter assay confirmed that SNHG7 could bind miR-186. Conclusions SNHG7 enhances drug resistance of AML cells during chemotherapy through inhibition of miR-186.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a200521.pdfsnhg7|mir-186|proliferation|drug-resistance|acute myeloid leukemia |
| spellingShingle | ZHU Juan, ZHOU Ying, LI Ying-jia LncRNA SNHG7 enhances the drug resistance of human acute myeloid leukemia cells through inhibiting miR-186 Jichu yixue yu linchuang snhg7|mir-186|proliferation|drug-resistance|acute myeloid leukemia |
| title | LncRNA SNHG7 enhances the drug resistance of human acute myeloid leukemia cells through inhibiting miR-186 |
| title_full | LncRNA SNHG7 enhances the drug resistance of human acute myeloid leukemia cells through inhibiting miR-186 |
| title_fullStr | LncRNA SNHG7 enhances the drug resistance of human acute myeloid leukemia cells through inhibiting miR-186 |
| title_full_unstemmed | LncRNA SNHG7 enhances the drug resistance of human acute myeloid leukemia cells through inhibiting miR-186 |
| title_short | LncRNA SNHG7 enhances the drug resistance of human acute myeloid leukemia cells through inhibiting miR-186 |
| title_sort | lncrna snhg7 enhances the drug resistance of human acute myeloid leukemia cells through inhibiting mir 186 |
| topic | snhg7|mir-186|proliferation|drug-resistance|acute myeloid leukemia |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a200521.pdf |
| work_keys_str_mv | AT zhujuanzhouyingliyingjia lncrnasnhg7enhancesthedrugresistanceofhumanacutemyeloidleukemiacellsthroughinhibitingmir186 |