Expression Patterns of MiR-125a and MiR-223 and Their Association with Diabetes Mellitus and Survival in Patients with Non-ST-Segment Elevation Acute Coronary Syndrome
<b>Background</b>: MicroRNAs (miRNA, miR) are small, non-coding RNAs which have become increasingly relevant as diagnostic and prognostic biomarkers. The objective of this study was the investigation of blood-derived miRNAs and their link to long-term all-cause mortality in patients who...
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MDPI AG
2023-04-01
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| Online Access: | https://www.mdpi.com/2227-9059/11/4/1118 |
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| author | Gloria M. Gager Ceren Eyileten Marek Postuła Anna Nowak Aleksandra Gąsecka Bernd Jilma Jolanta M. Siller-Matula |
| author_facet | Gloria M. Gager Ceren Eyileten Marek Postuła Anna Nowak Aleksandra Gąsecka Bernd Jilma Jolanta M. Siller-Matula |
| author_sort | Gloria M. Gager |
| collection | DOAJ |
| description | <b>Background</b>: MicroRNAs (miRNA, miR) are small, non-coding RNAs which have become increasingly relevant as diagnostic and prognostic biomarkers. The objective of this study was the investigation of blood-derived miRNAs and their link to long-term all-cause mortality in patients who suffered from non-ST-segment elevation acute coronary syndrome (NSTE-ACS). <b>Methods</b>: This study was an observational prospective study, which included 109 patients with NSTE-ACS. Analysis of the expression of miR-125a and miR-223 was conducted by polymerase chain reaction (PCR). The follow-up period comprised a median of 7.5 years. Long-term all-cause mortality was considered as the primary endpoint. Adjusted Cox-regression analysis was performed for prediction of events. <b>Results</b>: Increased expression of miR-223 (>7.1) at the time point of the event was related to improved long-term all-cause survival (adjusted (adj.) hazard ratio (HR) = 0.09, 95% confidence interval (95%CI): 0.01–0.75; <i>p</i> = 0.026). The receiver operating characteristic (ROC) analysis provided sufficient c-statistics (area under the curve (AUC) = 0.73, 95%CI: 0.58–0.86; <i>p</i> = 0.034; negative predictive value of 98%) for miR-223 to predict long-term all-cause survival. The Kaplan–Meier time to event analysis showed a separation of the survival curves between the groups at an early stage (log rank <i>p</i> = 0.015). Higher plasma miR-125a levels were found in patients with diabetes mellitus vs. in those without (<i>p</i> = 0.010). Furthermore, increased miR-125a expression was associated with an elevated HbA1c concentration. <b>Conclusions</b>: In this hypothesis-generating study, higher values of miR-223 were related to improved long-term survival in patients after NSTE-ACS. Larger studies are required in order to evaluate whether miR-223 can be used as a suitable predictor for long-term all-cause mortality. |
| first_indexed | 2024-03-11T05:12:42Z |
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| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-11T05:12:42Z |
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| spelling | doaj.art-0df68896e89c42f3bbb1c49fdce13fa82023-11-17T18:26:47ZengMDPI AGBiomedicines2227-90592023-04-01114111810.3390/biomedicines11041118Expression Patterns of MiR-125a and MiR-223 and Their Association with Diabetes Mellitus and Survival in Patients with Non-ST-Segment Elevation Acute Coronary SyndromeGloria M. Gager0Ceren Eyileten1Marek Postuła2Anna Nowak3Aleksandra Gąsecka4Bernd Jilma5Jolanta M. Siller-Matula6Division of Cardiology, Department of Internal Medicine II, Medical University of Vienna, 1090 Vienna, AustriaCentre for Preclinical Research and Technology (CEPT), Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, 02-091 Warsaw, PolandCentre for Preclinical Research and Technology (CEPT), Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, 02-091 Warsaw, PolandCentre for Preclinical Research and Technology (CEPT), Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, 02-091 Warsaw, PolandDepartment of Cardiology, Medical University of Warsaw, 02-091 Warsaw, PolandDepartment of Clinical Pharmacology, Medical University of Vienna, 1090 Vienna, AustriaDivision of Cardiology, Department of Internal Medicine II, Medical University of Vienna, 1090 Vienna, Austria<b>Background</b>: MicroRNAs (miRNA, miR) are small, non-coding RNAs which have become increasingly relevant as diagnostic and prognostic biomarkers. The objective of this study was the investigation of blood-derived miRNAs and their link to long-term all-cause mortality in patients who suffered from non-ST-segment elevation acute coronary syndrome (NSTE-ACS). <b>Methods</b>: This study was an observational prospective study, which included 109 patients with NSTE-ACS. Analysis of the expression of miR-125a and miR-223 was conducted by polymerase chain reaction (PCR). The follow-up period comprised a median of 7.5 years. Long-term all-cause mortality was considered as the primary endpoint. Adjusted Cox-regression analysis was performed for prediction of events. <b>Results</b>: Increased expression of miR-223 (>7.1) at the time point of the event was related to improved long-term all-cause survival (adjusted (adj.) hazard ratio (HR) = 0.09, 95% confidence interval (95%CI): 0.01–0.75; <i>p</i> = 0.026). The receiver operating characteristic (ROC) analysis provided sufficient c-statistics (area under the curve (AUC) = 0.73, 95%CI: 0.58–0.86; <i>p</i> = 0.034; negative predictive value of 98%) for miR-223 to predict long-term all-cause survival. The Kaplan–Meier time to event analysis showed a separation of the survival curves between the groups at an early stage (log rank <i>p</i> = 0.015). Higher plasma miR-125a levels were found in patients with diabetes mellitus vs. in those without (<i>p</i> = 0.010). Furthermore, increased miR-125a expression was associated with an elevated HbA1c concentration. <b>Conclusions</b>: In this hypothesis-generating study, higher values of miR-223 were related to improved long-term survival in patients after NSTE-ACS. Larger studies are required in order to evaluate whether miR-223 can be used as a suitable predictor for long-term all-cause mortality.https://www.mdpi.com/2227-9059/11/4/1118acute coronary syndromeNSTEMINSTE-ACSmicroRNAmiRNAmiR-125a |
| spellingShingle | Gloria M. Gager Ceren Eyileten Marek Postuła Anna Nowak Aleksandra Gąsecka Bernd Jilma Jolanta M. Siller-Matula Expression Patterns of MiR-125a and MiR-223 and Their Association with Diabetes Mellitus and Survival in Patients with Non-ST-Segment Elevation Acute Coronary Syndrome Biomedicines acute coronary syndrome NSTEMI NSTE-ACS microRNA miRNA miR-125a |
| title | Expression Patterns of MiR-125a and MiR-223 and Their Association with Diabetes Mellitus and Survival in Patients with Non-ST-Segment Elevation Acute Coronary Syndrome |
| title_full | Expression Patterns of MiR-125a and MiR-223 and Their Association with Diabetes Mellitus and Survival in Patients with Non-ST-Segment Elevation Acute Coronary Syndrome |
| title_fullStr | Expression Patterns of MiR-125a and MiR-223 and Their Association with Diabetes Mellitus and Survival in Patients with Non-ST-Segment Elevation Acute Coronary Syndrome |
| title_full_unstemmed | Expression Patterns of MiR-125a and MiR-223 and Their Association with Diabetes Mellitus and Survival in Patients with Non-ST-Segment Elevation Acute Coronary Syndrome |
| title_short | Expression Patterns of MiR-125a and MiR-223 and Their Association with Diabetes Mellitus and Survival in Patients with Non-ST-Segment Elevation Acute Coronary Syndrome |
| title_sort | expression patterns of mir 125a and mir 223 and their association with diabetes mellitus and survival in patients with non st segment elevation acute coronary syndrome |
| topic | acute coronary syndrome NSTEMI NSTE-ACS microRNA miRNA miR-125a |
| url | https://www.mdpi.com/2227-9059/11/4/1118 |
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