Transcriptome dataset of Babesia bovis life stages within vertebrate and invertebrate hosts
Babesia bovis is a hemoprotozoan parasite of cattle that has a complex life cycle within vertebrate and invertebrate hosts. In the mammalian host, B. bovis undergoes asexual reproduction while in the tick midgut, gametes are induced, fuse, and form zygotes. The zygote infects tick gut epithelial cel...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-12-01
|
| Series: | Data in Brief |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352340920314153 |
| _version_ | 1819060748556435456 |
|---|---|
| author | Massaro W. Ueti Wendell C. Johnson Lowell S. Kappmeyer David R. Herndon Michelle R. Mousel Kathryn E. Reif Naomi S. Taus Olukemi O. Ifeonu Joana C. Silva Carlos E. Suarez Kelly A. Brayton |
| author_facet | Massaro W. Ueti Wendell C. Johnson Lowell S. Kappmeyer David R. Herndon Michelle R. Mousel Kathryn E. Reif Naomi S. Taus Olukemi O. Ifeonu Joana C. Silva Carlos E. Suarez Kelly A. Brayton |
| author_sort | Massaro W. Ueti |
| collection | DOAJ |
| description | Babesia bovis is a hemoprotozoan parasite of cattle that has a complex life cycle within vertebrate and invertebrate hosts. In the mammalian host, B. bovis undergoes asexual reproduction while in the tick midgut, gametes are induced, fuse, and form zygotes. The zygote infects tick gut epithelial cells and transform into kinetes that are released into the hemolymph and invade other tick tissues such as the ovaries, resulting in transovarial transmission to tick offspring. To compare gene regulation between different B. bovis life stages, we collected parasites infecting bovine erythrocytes and tick hemolymph. Total RNA samples were isolated, and multiplexed libraries sequenced using paired-end 100 cycle reads of a HiSeq 2500. The data was normalized using the TMM method and analysed for significant differential expression using the generalized linear model likelihood ratio test (GLM LRT) in edgeR. To validate our datasets, ten genes were selected using NormFinder. Genes that had no significant fold change between the blood and tick stages in the RNA-Seq datasets were tested by quantitative PCR to determine their suitability as “housekeeping” genes. The normalized RNA-Seq data revealed genes upregulated during infection of the mammalian host or tick vector and six upregulated genes were validated by quantitative PCR. These datasets can help identify useful targets for controlling bovine babesiosis. |
| first_indexed | 2024-12-21T14:31:55Z |
| format | Article |
| id | doaj.art-0df6a8d27b8049958bfe10a9ca5479d1 |
| institution | Directory Open Access Journal |
| issn | 2352-3409 |
| language | English |
| last_indexed | 2024-12-21T14:31:55Z |
| publishDate | 2020-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj.art-0df6a8d27b8049958bfe10a9ca5479d12022-12-21T19:00:27ZengElsevierData in Brief2352-34092020-12-0133106533Transcriptome dataset of Babesia bovis life stages within vertebrate and invertebrate hostsMassaro W. Ueti0Wendell C. Johnson1Lowell S. Kappmeyer2David R. Herndon3Michelle R. Mousel4Kathryn E. Reif5Naomi S. Taus6Olukemi O. Ifeonu7Joana C. Silva8Carlos E. Suarez9Kelly A. Brayton10Animal Disease Research Unit, USDA-ARS, Pullman, WA, United States; Program in Vector-borne Disease, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, United States; Paul G. Allen School for Global Animal Health, Washington State University, Pullman, WA, United States; Corresponding author at: Animal Diseases Research Unit, USDA-ARS, Pullman, WA 99164-6630, United States.Animal Disease Research Unit, USDA-ARS, Pullman, WA, United StatesAnimal Disease Research Unit, USDA-ARS, Pullman, WA, United StatesAnimal Disease Research Unit, USDA-ARS, Pullman, WA, United StatesAnimal Disease Research Unit, USDA-ARS, Pullman, WA, United States; Paul G. Allen School for Global Animal Health, Washington State University, Pullman, WA, United StatesAnimal Disease Research Unit, USDA-ARS, Pullman, WA, United States; Program in Vector-borne Disease, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, United StatesAnimal Disease Research Unit, USDA-ARS, Pullman, WA, United States; Program in Vector-borne Disease, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, United StatesInstitute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD, United StatesInstitute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD, United States; Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD, United StatesAnimal Disease Research Unit, USDA-ARS, Pullman, WA, United States; Program in Vector-borne Disease, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, United StatesProgram in Vector-borne Disease, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, United States; Paul G. Allen School for Global Animal Health, Washington State University, Pullman, WA, United States; Corresponding author at: Program in Vector-borne Disease, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040, United States.Babesia bovis is a hemoprotozoan parasite of cattle that has a complex life cycle within vertebrate and invertebrate hosts. In the mammalian host, B. bovis undergoes asexual reproduction while in the tick midgut, gametes are induced, fuse, and form zygotes. The zygote infects tick gut epithelial cells and transform into kinetes that are released into the hemolymph and invade other tick tissues such as the ovaries, resulting in transovarial transmission to tick offspring. To compare gene regulation between different B. bovis life stages, we collected parasites infecting bovine erythrocytes and tick hemolymph. Total RNA samples were isolated, and multiplexed libraries sequenced using paired-end 100 cycle reads of a HiSeq 2500. The data was normalized using the TMM method and analysed for significant differential expression using the generalized linear model likelihood ratio test (GLM LRT) in edgeR. To validate our datasets, ten genes were selected using NormFinder. Genes that had no significant fold change between the blood and tick stages in the RNA-Seq datasets were tested by quantitative PCR to determine their suitability as “housekeeping” genes. The normalized RNA-Seq data revealed genes upregulated during infection of the mammalian host or tick vector and six upregulated genes were validated by quantitative PCR. These datasets can help identify useful targets for controlling bovine babesiosis.http://www.sciencedirect.com/science/article/pii/S2352340920314153Bovine babesiosisBabesiaBovineGene expressionKinetesRhipicephalus microplus |
| spellingShingle | Massaro W. Ueti Wendell C. Johnson Lowell S. Kappmeyer David R. Herndon Michelle R. Mousel Kathryn E. Reif Naomi S. Taus Olukemi O. Ifeonu Joana C. Silva Carlos E. Suarez Kelly A. Brayton Transcriptome dataset of Babesia bovis life stages within vertebrate and invertebrate hosts Data in Brief Bovine babesiosis Babesia Bovine Gene expression Kinetes Rhipicephalus microplus |
| title | Transcriptome dataset of Babesia bovis life stages within vertebrate and invertebrate hosts |
| title_full | Transcriptome dataset of Babesia bovis life stages within vertebrate and invertebrate hosts |
| title_fullStr | Transcriptome dataset of Babesia bovis life stages within vertebrate and invertebrate hosts |
| title_full_unstemmed | Transcriptome dataset of Babesia bovis life stages within vertebrate and invertebrate hosts |
| title_short | Transcriptome dataset of Babesia bovis life stages within vertebrate and invertebrate hosts |
| title_sort | transcriptome dataset of babesia bovis life stages within vertebrate and invertebrate hosts |
| topic | Bovine babesiosis Babesia Bovine Gene expression Kinetes Rhipicephalus microplus |
| url | http://www.sciencedirect.com/science/article/pii/S2352340920314153 |
| work_keys_str_mv | AT massarowueti transcriptomedatasetofbabesiabovislifestageswithinvertebrateandinvertebratehosts AT wendellcjohnson transcriptomedatasetofbabesiabovislifestageswithinvertebrateandinvertebratehosts AT lowellskappmeyer transcriptomedatasetofbabesiabovislifestageswithinvertebrateandinvertebratehosts AT davidrherndon transcriptomedatasetofbabesiabovislifestageswithinvertebrateandinvertebratehosts AT michellermousel transcriptomedatasetofbabesiabovislifestageswithinvertebrateandinvertebratehosts AT kathrynereif transcriptomedatasetofbabesiabovislifestageswithinvertebrateandinvertebratehosts AT naomistaus transcriptomedatasetofbabesiabovislifestageswithinvertebrateandinvertebratehosts AT olukemioifeonu transcriptomedatasetofbabesiabovislifestageswithinvertebrateandinvertebratehosts AT joanacsilva transcriptomedatasetofbabesiabovislifestageswithinvertebrateandinvertebratehosts AT carlosesuarez transcriptomedatasetofbabesiabovislifestageswithinvertebrateandinvertebratehosts AT kellyabrayton transcriptomedatasetofbabesiabovislifestageswithinvertebrateandinvertebratehosts |