Two Distinct Soil Disinfestations Differently Modify the Bacterial Communities in a Tomato Field
Reductive soil disinfestation (RSD) and soil solarization (SS) were evaluated based on environmental factors, microbiome, and suppression of <i>Fusarium oxysporum</i> in a tomato field soil. Soil environmental factors (moisture content, electric conductivity, pH, and redox potential (RP)...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-07-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/11/7/1375 |
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| author | Masaru Nakayasu Kyoko Ikeda Shinichi Yamazaki Yuichi Aoki Kazufumi Yazaki Haruhiko Washida Akifumi Sugiyama |
| author_facet | Masaru Nakayasu Kyoko Ikeda Shinichi Yamazaki Yuichi Aoki Kazufumi Yazaki Haruhiko Washida Akifumi Sugiyama |
| author_sort | Masaru Nakayasu |
| collection | DOAJ |
| description | Reductive soil disinfestation (RSD) and soil solarization (SS) were evaluated based on environmental factors, microbiome, and suppression of <i>Fusarium oxysporum</i> in a tomato field soil. Soil environmental factors (moisture content, electric conductivity, pH, and redox potential (RP)) were measured during soil disinfestations. All factors were more strongly influenced by RSD than SS. 16S rRNA amplicon sequencing of RSD- and SS-treated soils was performed. The bacterial communities were taxonomically and functionally distinct depending on treatment methods and periods and significantly correlated with pH and RP. Fifty-four pathways predicted by PICRUSt2 (third level in MetaCyc hierarchy) were significantly different between RSD and SS. Quantitative polymerase chain reaction demonstrated that both treatments equally suppressed <i>F. oxysporum</i>. The growth and yield of tomato cultivated after treatments were similar between RSD and SS. RSD and SS shaped different soil bacterial communities, although the effects on pathogen suppression and tomato plant growth were comparable between treatments. The existence of pathogen-suppressive microbes, other than Clostridia previously reported to have an effect, was suggested. Comparison between RSD and SS provides new aspects of unknown disinfestation patterns and the usefulness of SS as an alternative to RSD. |
| first_indexed | 2024-03-10T09:48:32Z |
| format | Article |
| id | doaj.art-0dfcb44f664b48b58d904284e767047b |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T09:48:32Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-0dfcb44f664b48b58d904284e767047b2023-11-22T02:58:10ZengMDPI AGAgronomy2073-43952021-07-01117137510.3390/agronomy11071375Two Distinct Soil Disinfestations Differently Modify the Bacterial Communities in a Tomato FieldMasaru Nakayasu0Kyoko Ikeda1Shinichi Yamazaki2Yuichi Aoki3Kazufumi Yazaki4Haruhiko Washida5Akifumi Sugiyama6Research Institute for Sustainable Humanosphere, Kyoto University, Gokasho, Uji 611-0011, JapanOrganic Nico Co., Ltd., Oharanohaigata, Nishikyo-ku, Kyoto 610-1132, JapanTohoku Medical Megabank Organization, Tohoku University, Sendai 980-8573, JapanTohoku Medical Megabank Organization, Tohoku University, Sendai 980-8573, JapanResearch Institute for Sustainable Humanosphere, Kyoto University, Gokasho, Uji 611-0011, JapanOrganic Nico Co., Ltd., Oharanohaigata, Nishikyo-ku, Kyoto 610-1132, JapanResearch Institute for Sustainable Humanosphere, Kyoto University, Gokasho, Uji 611-0011, JapanReductive soil disinfestation (RSD) and soil solarization (SS) were evaluated based on environmental factors, microbiome, and suppression of <i>Fusarium oxysporum</i> in a tomato field soil. Soil environmental factors (moisture content, electric conductivity, pH, and redox potential (RP)) were measured during soil disinfestations. All factors were more strongly influenced by RSD than SS. 16S rRNA amplicon sequencing of RSD- and SS-treated soils was performed. The bacterial communities were taxonomically and functionally distinct depending on treatment methods and periods and significantly correlated with pH and RP. Fifty-four pathways predicted by PICRUSt2 (third level in MetaCyc hierarchy) were significantly different between RSD and SS. Quantitative polymerase chain reaction demonstrated that both treatments equally suppressed <i>F. oxysporum</i>. The growth and yield of tomato cultivated after treatments were similar between RSD and SS. RSD and SS shaped different soil bacterial communities, although the effects on pathogen suppression and tomato plant growth were comparable between treatments. The existence of pathogen-suppressive microbes, other than Clostridia previously reported to have an effect, was suggested. Comparison between RSD and SS provides new aspects of unknown disinfestation patterns and the usefulness of SS as an alternative to RSD.https://www.mdpi.com/2073-4395/11/7/1375bacterial communities<i>Fusarium oxysporum</i>reductive soil infestationsoil solarizationtomato |
| spellingShingle | Masaru Nakayasu Kyoko Ikeda Shinichi Yamazaki Yuichi Aoki Kazufumi Yazaki Haruhiko Washida Akifumi Sugiyama Two Distinct Soil Disinfestations Differently Modify the Bacterial Communities in a Tomato Field Agronomy bacterial communities <i>Fusarium oxysporum</i> reductive soil infestation soil solarization tomato |
| title | Two Distinct Soil Disinfestations Differently Modify the Bacterial Communities in a Tomato Field |
| title_full | Two Distinct Soil Disinfestations Differently Modify the Bacterial Communities in a Tomato Field |
| title_fullStr | Two Distinct Soil Disinfestations Differently Modify the Bacterial Communities in a Tomato Field |
| title_full_unstemmed | Two Distinct Soil Disinfestations Differently Modify the Bacterial Communities in a Tomato Field |
| title_short | Two Distinct Soil Disinfestations Differently Modify the Bacterial Communities in a Tomato Field |
| title_sort | two distinct soil disinfestations differently modify the bacterial communities in a tomato field |
| topic | bacterial communities <i>Fusarium oxysporum</i> reductive soil infestation soil solarization tomato |
| url | https://www.mdpi.com/2073-4395/11/7/1375 |
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