Pan-cancer analysis and in vitro validation of the oncogenic and prognostic roles of AURKA in human cancers
BackgroundAurora kinase A (AURKA) plays a pivotal role in regulating cell mitosis and tumor progression. However, its prognostic significance across diverse cancer types remains relatively unexplored.MethodsWe conducted a comprehensive analysis of AURKA expression in various cancers using data from...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-10-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2023.1186101/full |
| _version_ | 1797646895648079872 |
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| author | Chuang Yang Patrick Sven Plum Ines Gockel René Thieme |
| author_facet | Chuang Yang Patrick Sven Plum Ines Gockel René Thieme |
| author_sort | Chuang Yang |
| collection | DOAJ |
| description | BackgroundAurora kinase A (AURKA) plays a pivotal role in regulating cell mitosis and tumor progression. However, its prognostic significance across diverse cancer types remains relatively unexplored.MethodsWe conducted a comprehensive analysis of AURKA expression in various cancers using data from The Cancer Genome Atlas, Genotype-Tissue Expression, and The Human Protein Atlas databases. Our investigation encompassed an exploration of the associations between AURKA expression and clinical characteristics, shedding light on potential functional roles of AURKA. Additionally, we delved into the relationship between AURKA and the tumor microenvironment. To substantiate the role of AURKA, we carried out in vitro experiments in esophageal adenocarcinoma (EAC), prostate cancer (PRAD), and pancreatic cancer (PAAD) cells.ResultsOur analysis revealed that AURKA is prominently overexpressed in a majority of the cancer types under investigation. Elevated AURKA expression correlated closely with poorer prognosis and advanced tumor stages. AURKA was found to be associated with key pathways involved in the cell cycle and arachidonic acid metabolism. Moreover, AURKA expression exhibited significant correlations with immunoregulatory genes and immune cell profiles. Notably, in vitro experiments demonstrated that silencing AURKA expression resulted in reduced cell viability in EAC, PRAD, and PAAD cells, as well as a decrease in clone formation, cell cycle elongation, diminished cell invasion and reduced spheroid size in EAC cells (OE33 and OE19).ConclusionOur study elucidates the oncogenic role of AURKA and underscores its prognostic value across a spectrum of cancers, including EAC. These findings suggest that AURKA holds promise as a predictive biomarker for EAC and various other tumor types. |
| first_indexed | 2024-03-11T15:09:27Z |
| format | Article |
| id | doaj.art-0dfd07a4a6704ebe81c3e31c96aee53b |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-03-11T15:09:27Z |
| publishDate | 2023-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-0dfd07a4a6704ebe81c3e31c96aee53b2023-10-29T17:13:26ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2023-10-011310.3389/fonc.2023.11861011186101Pan-cancer analysis and in vitro validation of the oncogenic and prognostic roles of AURKA in human cancersChuang YangPatrick Sven PlumInes GockelRené ThiemeBackgroundAurora kinase A (AURKA) plays a pivotal role in regulating cell mitosis and tumor progression. However, its prognostic significance across diverse cancer types remains relatively unexplored.MethodsWe conducted a comprehensive analysis of AURKA expression in various cancers using data from The Cancer Genome Atlas, Genotype-Tissue Expression, and The Human Protein Atlas databases. Our investigation encompassed an exploration of the associations between AURKA expression and clinical characteristics, shedding light on potential functional roles of AURKA. Additionally, we delved into the relationship between AURKA and the tumor microenvironment. To substantiate the role of AURKA, we carried out in vitro experiments in esophageal adenocarcinoma (EAC), prostate cancer (PRAD), and pancreatic cancer (PAAD) cells.ResultsOur analysis revealed that AURKA is prominently overexpressed in a majority of the cancer types under investigation. Elevated AURKA expression correlated closely with poorer prognosis and advanced tumor stages. AURKA was found to be associated with key pathways involved in the cell cycle and arachidonic acid metabolism. Moreover, AURKA expression exhibited significant correlations with immunoregulatory genes and immune cell profiles. Notably, in vitro experiments demonstrated that silencing AURKA expression resulted in reduced cell viability in EAC, PRAD, and PAAD cells, as well as a decrease in clone formation, cell cycle elongation, diminished cell invasion and reduced spheroid size in EAC cells (OE33 and OE19).ConclusionOur study elucidates the oncogenic role of AURKA and underscores its prognostic value across a spectrum of cancers, including EAC. These findings suggest that AURKA holds promise as a predictive biomarker for EAC and various other tumor types.https://www.frontiersin.org/articles/10.3389/fonc.2023.1186101/fullAURKApan-canceresophageal adenocarcinoma (EAC)biomarkerspheroids |
| spellingShingle | Chuang Yang Patrick Sven Plum Ines Gockel René Thieme Pan-cancer analysis and in vitro validation of the oncogenic and prognostic roles of AURKA in human cancers Frontiers in Oncology AURKA pan-cancer esophageal adenocarcinoma (EAC) biomarker spheroids |
| title | Pan-cancer analysis and in vitro validation of the oncogenic and prognostic roles of AURKA in human cancers |
| title_full | Pan-cancer analysis and in vitro validation of the oncogenic and prognostic roles of AURKA in human cancers |
| title_fullStr | Pan-cancer analysis and in vitro validation of the oncogenic and prognostic roles of AURKA in human cancers |
| title_full_unstemmed | Pan-cancer analysis and in vitro validation of the oncogenic and prognostic roles of AURKA in human cancers |
| title_short | Pan-cancer analysis and in vitro validation of the oncogenic and prognostic roles of AURKA in human cancers |
| title_sort | pan cancer analysis and in vitro validation of the oncogenic and prognostic roles of aurka in human cancers |
| topic | AURKA pan-cancer esophageal adenocarcinoma (EAC) biomarker spheroids |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2023.1186101/full |
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