Effect of UBE2Q on BECN and CEA Protein in Colorectal Cancer Cells
Background: Expression of UBE2Q1, UBE2Q2, and members of the ubiquitin-conjugating enzyme family (E2) are affected in colorectal cancer (CRC). The BECN gene plays a key role in CRC cells. In gastrointestinal carcinoma therapy, tumor-associated antigens such as CEA are typically used.To investigate t...
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| Format: | Article |
| Language: | English |
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Mazandaran University of Medical Sciences and Health Services
2018-12-01
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| Series: | Research in Molecular Medicine |
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| Online Access: | https://doi.org/10.18502/rmm.v6i2.4535 |
| _version_ | 1818281052998729728 |
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| author | Niloofar Boroumand |
| author_facet | Niloofar Boroumand |
| author_sort | Niloofar Boroumand |
| collection | DOAJ |
| description | Background: Expression of UBE2Q1, UBE2Q2, and members of the ubiquitin-conjugating enzyme family (E2) are affected in colorectal cancer (CRC). The BECN gene plays a key role in CRC cells. In gastrointestinal carcinoma therapy, tumor-associated antigens such as CEA are typically used.To investigate the association between UBE2Q1 and Beclin1 autophagy marker and CEA protein expression in LS180 CRC cell line.Materials and Methods: In this study, changes in the expression of BECN marker in LS180 cell lines with the vector containing UBE2Q1 were investigated using real-time PCR. The expression of CEA protein was also evaluated by western blotting. Statistical analyses were performed with Graph Pad Prism software.Results: The results indicated reduced expression of BECN autophagy marker (P=0). Therefore, in the presence of UBE2Q1, cancer cells have less ability to induce autophagy. However, CEA protein levels in LS180 transfected cells with a UBE2Q1-ORF-containing plasmid decreased when compared to non-transfected cells.Conclusion: The use of pharmacologic inhibitors related to the autophagy mechanism can be a novel approach in cancer therapy. |
| first_indexed | 2024-12-12T23:59:00Z |
| format | Article |
| id | doaj.art-0dfe0aed6b27423199e07525ebc3f776 |
| institution | Directory Open Access Journal |
| issn | 2322-1348 |
| language | English |
| last_indexed | 2024-12-12T23:59:00Z |
| publishDate | 2018-12-01 |
| publisher | Mazandaran University of Medical Sciences and Health Services |
| record_format | Article |
| series | Research in Molecular Medicine |
| spelling | doaj.art-0dfe0aed6b27423199e07525ebc3f7762022-12-22T00:06:28ZengMazandaran University of Medical Sciences and Health ServicesResearch in Molecular Medicine2322-13482018-12-0120171710.18502/rmm.v6i2.4535rmm.v6i2.4535Effect of UBE2Q on BECN and CEA Protein in Colorectal Cancer CellsNiloofar Boroumand0Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, IranBackground: Expression of UBE2Q1, UBE2Q2, and members of the ubiquitin-conjugating enzyme family (E2) are affected in colorectal cancer (CRC). The BECN gene plays a key role in CRC cells. In gastrointestinal carcinoma therapy, tumor-associated antigens such as CEA are typically used.To investigate the association between UBE2Q1 and Beclin1 autophagy marker and CEA protein expression in LS180 CRC cell line.Materials and Methods: In this study, changes in the expression of BECN marker in LS180 cell lines with the vector containing UBE2Q1 were investigated using real-time PCR. The expression of CEA protein was also evaluated by western blotting. Statistical analyses were performed with Graph Pad Prism software.Results: The results indicated reduced expression of BECN autophagy marker (P=0). Therefore, in the presence of UBE2Q1, cancer cells have less ability to induce autophagy. However, CEA protein levels in LS180 transfected cells with a UBE2Q1-ORF-containing plasmid decreased when compared to non-transfected cells.Conclusion: The use of pharmacologic inhibitors related to the autophagy mechanism can be a novel approach in cancer therapy.https://doi.org/10.18502/rmm.v6i2.4535BECN, CEA protein, colorectal cancer |
| spellingShingle | Niloofar Boroumand Effect of UBE2Q on BECN and CEA Protein in Colorectal Cancer Cells Research in Molecular Medicine BECN, CEA protein, colorectal cancer |
| title | Effect of UBE2Q on BECN and CEA Protein in Colorectal Cancer Cells |
| title_full | Effect of UBE2Q on BECN and CEA Protein in Colorectal Cancer Cells |
| title_fullStr | Effect of UBE2Q on BECN and CEA Protein in Colorectal Cancer Cells |
| title_full_unstemmed | Effect of UBE2Q on BECN and CEA Protein in Colorectal Cancer Cells |
| title_short | Effect of UBE2Q on BECN and CEA Protein in Colorectal Cancer Cells |
| title_sort | effect of ube2q on becn and cea protein in colorectal cancer cells |
| topic | BECN, CEA protein, colorectal cancer |
| url | https://doi.org/10.18502/rmm.v6i2.4535 |
| work_keys_str_mv | AT niloofarboroumand effectofube2qonbecnandceaproteinincolorectalcancercells |