Tumor HPV Status, Level of Regulatory T Cells and Macrophage Infiltration Predict up to 20-Year Non-Disease-Specific Survival in Oropharynx Squamous Cell Carcinoma Patients
Oropharynx squamous cell carcinoma (OPSCC) is of special interest because human papilloma virus (HPV) and/or smoking cause this disease. Influxes of inflammatory cells into such tumors are known to vary with prognoses. Aims: To study whether the density of tumor-infiltrating T lymphocytes and tumor-...
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MDPI AG
2022-10-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/10/10/2484 |
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| author | Hilde Haave Borghild Ljokjel Helene Lybak Svein E. Moe Jan E. Berge Olav K. Vintermyr Lars Helgeland Hans J. Aarstad |
| author_facet | Hilde Haave Borghild Ljokjel Helene Lybak Svein E. Moe Jan E. Berge Olav K. Vintermyr Lars Helgeland Hans J. Aarstad |
| author_sort | Hilde Haave |
| collection | DOAJ |
| description | Oropharynx squamous cell carcinoma (OPSCC) is of special interest because human papilloma virus (HPV) and/or smoking cause this disease. Influxes of inflammatory cells into such tumors are known to vary with prognoses. Aims: To study whether the density of tumor-infiltrating T lymphocytes and tumor-infiltrating macrophages predicted general 20-year overall survival (OS), as well as OS with only disease-specific survival (DSS) patients included. Methods: Biopsies from patients treated for OPSCC (<i>n</i> = 180) were stained by immunohistochemistry and the tumor cell macrophage (CD68), pan T lymphocytes (CD3), and regulatory T lymphocytes (Foxp3) densities were determined. The HE-determined percentage of matured tumor cells and the rate of invasion were calculated, and stromal desmoplasia were performed. Tumor HPV presence was studied by PCR. Twenty-year OS and five-year DSS patients were determined. Results: Tumor HPV status strongly predicted survival. High tumor infiltration of CD3, Foxp3 and CD68-positive cells predicted better twenty-year OS, with and without HPV stratification. Foxp3 and CD68 levels predicted OS, and 20-year among DSS patients, primarily among HPV(+) patients. Tumor HE-derived variables did not predict such survival. Conclusions: Tumor HPV status, level of Foxp3 tumor-infiltrating lymphocytes and CD68 tumor-infiltrating macrophages predicted up to 20-year OS of both all patients and disease-specific survived patients. |
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| institution | Directory Open Access Journal |
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| last_indexed | 2024-03-09T20:38:33Z |
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| series | Biomedicines |
| spelling | doaj.art-0dff1e7f106d407d928f029229c807802023-11-23T23:03:48ZengMDPI AGBiomedicines2227-90592022-10-011010248410.3390/biomedicines10102484Tumor HPV Status, Level of Regulatory T Cells and Macrophage Infiltration Predict up to 20-Year Non-Disease-Specific Survival in Oropharynx Squamous Cell Carcinoma PatientsHilde Haave0Borghild Ljokjel1Helene Lybak2Svein E. Moe3Jan E. Berge4Olav K. Vintermyr5Lars Helgeland6Hans J. Aarstad7Department of Otolaryngology/Head and Neck Surgery, Haukeland University Hospital, 5020 Bergen, NorwayDepartment of Otolaryngology/Head and Neck Surgery, Haukeland University Hospital, 5020 Bergen, NorwayDepartment of Otolaryngology/Head and Neck Surgery, Haukeland University Hospital, 5020 Bergen, NorwayDepartment of Otolaryngology/Head and Neck Surgery, Haukeland University Hospital, 5020 Bergen, NorwayDepartment of Otolaryngology/Head and Neck Surgery, Haukeland University Hospital, 5020 Bergen, NorwayDepartment of Pathology, Haukeland University Hospital, 5020 Bergen, NorwayDepartment of Pathology, Haukeland University Hospital, 5020 Bergen, NorwayDepartment of Otolaryngology/Head and Neck Surgery, Haukeland University Hospital, 5020 Bergen, NorwayOropharynx squamous cell carcinoma (OPSCC) is of special interest because human papilloma virus (HPV) and/or smoking cause this disease. Influxes of inflammatory cells into such tumors are known to vary with prognoses. Aims: To study whether the density of tumor-infiltrating T lymphocytes and tumor-infiltrating macrophages predicted general 20-year overall survival (OS), as well as OS with only disease-specific survival (DSS) patients included. Methods: Biopsies from patients treated for OPSCC (<i>n</i> = 180) were stained by immunohistochemistry and the tumor cell macrophage (CD68), pan T lymphocytes (CD3), and regulatory T lymphocytes (Foxp3) densities were determined. The HE-determined percentage of matured tumor cells and the rate of invasion were calculated, and stromal desmoplasia were performed. Tumor HPV presence was studied by PCR. Twenty-year OS and five-year DSS patients were determined. Results: Tumor HPV status strongly predicted survival. High tumor infiltration of CD3, Foxp3 and CD68-positive cells predicted better twenty-year OS, with and without HPV stratification. Foxp3 and CD68 levels predicted OS, and 20-year among DSS patients, primarily among HPV(+) patients. Tumor HE-derived variables did not predict such survival. Conclusions: Tumor HPV status, level of Foxp3 tumor-infiltrating lymphocytes and CD68 tumor-infiltrating macrophages predicted up to 20-year OS of both all patients and disease-specific survived patients.https://www.mdpi.com/2227-9059/10/10/2484cancerimmunologyoropharynx |
| spellingShingle | Hilde Haave Borghild Ljokjel Helene Lybak Svein E. Moe Jan E. Berge Olav K. Vintermyr Lars Helgeland Hans J. Aarstad Tumor HPV Status, Level of Regulatory T Cells and Macrophage Infiltration Predict up to 20-Year Non-Disease-Specific Survival in Oropharynx Squamous Cell Carcinoma Patients Biomedicines cancer immunology oropharynx |
| title | Tumor HPV Status, Level of Regulatory T Cells and Macrophage Infiltration Predict up to 20-Year Non-Disease-Specific Survival in Oropharynx Squamous Cell Carcinoma Patients |
| title_full | Tumor HPV Status, Level of Regulatory T Cells and Macrophage Infiltration Predict up to 20-Year Non-Disease-Specific Survival in Oropharynx Squamous Cell Carcinoma Patients |
| title_fullStr | Tumor HPV Status, Level of Regulatory T Cells and Macrophage Infiltration Predict up to 20-Year Non-Disease-Specific Survival in Oropharynx Squamous Cell Carcinoma Patients |
| title_full_unstemmed | Tumor HPV Status, Level of Regulatory T Cells and Macrophage Infiltration Predict up to 20-Year Non-Disease-Specific Survival in Oropharynx Squamous Cell Carcinoma Patients |
| title_short | Tumor HPV Status, Level of Regulatory T Cells and Macrophage Infiltration Predict up to 20-Year Non-Disease-Specific Survival in Oropharynx Squamous Cell Carcinoma Patients |
| title_sort | tumor hpv status level of regulatory t cells and macrophage infiltration predict up to 20 year non disease specific survival in oropharynx squamous cell carcinoma patients |
| topic | cancer immunology oropharynx |
| url | https://www.mdpi.com/2227-9059/10/10/2484 |
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