Influenza A(H1N1)pdm09 Virus Alters Expression of Endothelial Factors in Pulmonary Vascular Endothelium in Rats
Influenza virus infection may cause endothelial activation and dysfunction. However, it is still not known to what extent the influenza virus can dysregulate the expression of various endothelial proteins. The aim of the study is to identify the level of expression of endothelial nitric oxide syntha...
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MDPI AG
2022-11-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/11/2518 |
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| author | Vladimir Marchenko Darya Mukhametdinova Irina Amosova Dmitry Lioznov Irina Zhilinskaya |
| author_facet | Vladimir Marchenko Darya Mukhametdinova Irina Amosova Dmitry Lioznov Irina Zhilinskaya |
| author_sort | Vladimir Marchenko |
| collection | DOAJ |
| description | Influenza virus infection may cause endothelial activation and dysfunction. However, it is still not known to what extent the influenza virus can dysregulate the expression of various endothelial proteins. The aim of the study is to identify the level of expression of endothelial nitric oxide synthase (eNOS), plasminogen activator inhibitor-1 (PAI-1), and tissue plasminogen activator (tPA) in the pulmonary vascular endothelium, as well as the concentration of PAI-1 and tPA in the blood plasma in Wistar rats. Animals were intranasally infected with rat-adapted influenza A(H1N1)pdm09 virus. The expression of eNOS, PAI-1 and tPA in the pulmonary vascular endothelium was determined by immunohistochemistry; the concentration of PAI-1 and tPA was analyzed by ELISA at 24 and 96 h post infection (hpi). Thus, the expression of eNOS in the pulmonary vascular endothelium decreased by 1.9-fold at 24 hpi and increased by 2-fold at 96 hpi. The expression of PAI-1 in the pulmonary vascular endothelium increased by 5.23-fold and 6.54-fold at 24 and 96 hpi, respectively. The concentration of PAI-1 in the blood plasma of the rats decreased by 3.84-fold at 96 hpi, but not at 24 hpi. The expression of tPA in the pulmonary vascular endothelium was increased 2.2-fold at 96 hpi. The obtained data indicate the development of endothelial dysfunction that is characterized by the dysregulation of endothelial protein expression in non-lethal and clinically non-severe experimental influenza virus infection. |
| first_indexed | 2024-03-09T17:55:51Z |
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| id | doaj.art-0e002b52e8d4439fb77261c60c322e61 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T17:55:51Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-0e002b52e8d4439fb77261c60c322e612023-11-24T10:18:30ZengMDPI AGViruses1999-49152022-11-011411251810.3390/v14112518Influenza A(H1N1)pdm09 Virus Alters Expression of Endothelial Factors in Pulmonary Vascular Endothelium in RatsVladimir Marchenko0Darya Mukhametdinova1Irina Amosova2Dmitry Lioznov3Irina Zhilinskaya4Smorodintsev Research Institute of Influenza, Russian Ministry of Health, 197376 St. Petersburg, RussiaAlmazov National Medical Research Centre, Russian Ministry of Health, 197341 St. Petersburg, RussiaSmorodintsev Research Institute of Influenza, Russian Ministry of Health, 197376 St. Petersburg, RussiaSmorodintsev Research Institute of Influenza, Russian Ministry of Health, 197376 St. Petersburg, RussiaSmorodintsev Research Institute of Influenza, Russian Ministry of Health, 197376 St. Petersburg, RussiaInfluenza virus infection may cause endothelial activation and dysfunction. However, it is still not known to what extent the influenza virus can dysregulate the expression of various endothelial proteins. The aim of the study is to identify the level of expression of endothelial nitric oxide synthase (eNOS), plasminogen activator inhibitor-1 (PAI-1), and tissue plasminogen activator (tPA) in the pulmonary vascular endothelium, as well as the concentration of PAI-1 and tPA in the blood plasma in Wistar rats. Animals were intranasally infected with rat-adapted influenza A(H1N1)pdm09 virus. The expression of eNOS, PAI-1 and tPA in the pulmonary vascular endothelium was determined by immunohistochemistry; the concentration of PAI-1 and tPA was analyzed by ELISA at 24 and 96 h post infection (hpi). Thus, the expression of eNOS in the pulmonary vascular endothelium decreased by 1.9-fold at 24 hpi and increased by 2-fold at 96 hpi. The expression of PAI-1 in the pulmonary vascular endothelium increased by 5.23-fold and 6.54-fold at 24 and 96 hpi, respectively. The concentration of PAI-1 in the blood plasma of the rats decreased by 3.84-fold at 96 hpi, but not at 24 hpi. The expression of tPA in the pulmonary vascular endothelium was increased 2.2-fold at 96 hpi. The obtained data indicate the development of endothelial dysfunction that is characterized by the dysregulation of endothelial protein expression in non-lethal and clinically non-severe experimental influenza virus infection.https://www.mdpi.com/1999-4915/14/11/2518Wistar ratsinfluenza virus A(H1N1)pdm09endothelial proteinspulmonary vascular endotheliumplasmaimmunohistochemistry |
| spellingShingle | Vladimir Marchenko Darya Mukhametdinova Irina Amosova Dmitry Lioznov Irina Zhilinskaya Influenza A(H1N1)pdm09 Virus Alters Expression of Endothelial Factors in Pulmonary Vascular Endothelium in Rats Viruses Wistar rats influenza virus A(H1N1)pdm09 endothelial proteins pulmonary vascular endothelium plasma immunohistochemistry |
| title | Influenza A(H1N1)pdm09 Virus Alters Expression of Endothelial Factors in Pulmonary Vascular Endothelium in Rats |
| title_full | Influenza A(H1N1)pdm09 Virus Alters Expression of Endothelial Factors in Pulmonary Vascular Endothelium in Rats |
| title_fullStr | Influenza A(H1N1)pdm09 Virus Alters Expression of Endothelial Factors in Pulmonary Vascular Endothelium in Rats |
| title_full_unstemmed | Influenza A(H1N1)pdm09 Virus Alters Expression of Endothelial Factors in Pulmonary Vascular Endothelium in Rats |
| title_short | Influenza A(H1N1)pdm09 Virus Alters Expression of Endothelial Factors in Pulmonary Vascular Endothelium in Rats |
| title_sort | influenza a h1n1 pdm09 virus alters expression of endothelial factors in pulmonary vascular endothelium in rats |
| topic | Wistar rats influenza virus A(H1N1)pdm09 endothelial proteins pulmonary vascular endothelium plasma immunohistochemistry |
| url | https://www.mdpi.com/1999-4915/14/11/2518 |
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