Metabolites Profiling and Bioassays Reveal <i>Bassia indica</i> Ethanol Extract Protective Effect against Stomach Ulcers Development via HMGB1/TLR-4/NF-κB Pathway
Clinical manifestation of gastric ulcers is frequent, in addition to their costly drug regimens, warranting the development of novel drugs at lower costs. Although <i>Bassia indica</i> is well characterized for its anti-inflammatory and antioxidant potential, capacity of its ethanol extr...
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| Format: | Article |
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MDPI AG
2023-06-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/6/1263 |
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| author | Zeinab A. El-Gendy Rehab F. Taher Abdelbaset M. Elgamal Ahmed Serag Azza Hassan Gehad A. Abdel Jaleel Mohamed A. Farag Abdelsamed I. Elshamy |
| author_facet | Zeinab A. El-Gendy Rehab F. Taher Abdelbaset M. Elgamal Ahmed Serag Azza Hassan Gehad A. Abdel Jaleel Mohamed A. Farag Abdelsamed I. Elshamy |
| author_sort | Zeinab A. El-Gendy |
| collection | DOAJ |
| description | Clinical manifestation of gastric ulcers is frequent, in addition to their costly drug regimens, warranting the development of novel drugs at lower costs. Although <i>Bassia indica</i> is well characterized for its anti-inflammatory and antioxidant potential, capacity of its ethanol extract (BIEE) to prevent stomach ulcers’ progression has not been reported. A nuclear protein termed high-mobility group box 1 (HMGB1) plays a key role in the formation of stomach ulcers by triggering a number of inflammatory responses. The main purpose of the current investigation was to evaluate the in vivo anti-inflammatory and anti-ulcerogenic capabilities of BIEE against ethanol-induced gastric ulcers in rats via the HMGB1/TLR-4/NF-B signaling pathway. HMGB1 and Nuclear factor kappa (NF-B) expression, IL-1β and Nrf2 contents showed an increase along with ulcer development, concurrent with an increase in immunohistochemical TLR-4 level. In contrast, pre-treatment with BIEE significantly reduced HMGB1 and Nuclear factor kappa (NF-B) expression levels, IL-1β and Nrf2 contents and ulcer index value. Such protective action was further confirmed based on histological and immunohistochemical TLR-4 assays. Untargeted analysis via UPLC-ESI–Qtof-MS has allowed for the comprehensive characterization of 40 metabolites in BIEE mostly belonged to two main chemical classes, <i>viz</i>., flavonoids and lipids. These key metabolites, particularly flavonoids, suggesting a mediation for the anti-inflammatory and anti-ulcerogenic properties of BIEE, pose it as a promising natural drug regimen for treatment of stomach ulcers. |
| first_indexed | 2024-03-11T02:49:20Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-11T02:49:20Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-0e02462b64b74905a18feb3e72abbc672023-11-18T09:03:44ZengMDPI AGAntioxidants2076-39212023-06-01126126310.3390/antiox12061263Metabolites Profiling and Bioassays Reveal <i>Bassia indica</i> Ethanol Extract Protective Effect against Stomach Ulcers Development via HMGB1/TLR-4/NF-κB PathwayZeinab A. El-Gendy0Rehab F. Taher1Abdelbaset M. Elgamal2Ahmed Serag3Azza Hassan4Gehad A. Abdel Jaleel5Mohamed A. Farag6Abdelsamed I. Elshamy7Department of Pharmacology, Medical Research and Clinical Studies Institute, National Research Centre, 33 El Bohouth St., Dokki, Giza 12622, EgyptDepartment of Natural Compounds Chemistry, National Research Center, 33 El Bohouth St., Dokki, Giza 12622, EgyptDepartment of Chemistry of Microbial and Natural Products, National Research Centre, 33 El-Bohouth St., Dokki, Giza 12622, EgyptPharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, Cairo 11751, EgyptDepartment of Pathology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, EgyptDepartment of Pharmacology, Medical Research and Clinical Studies Institute, National Research Centre, 33 El Bohouth St., Dokki, Giza 12622, EgyptPharmacognosy Department, Faculty of Pharmacy, Cairo University, Kasr el Aini St., Cairo 11562, EgyptDepartment of Natural Compounds Chemistry, National Research Center, 33 El Bohouth St., Dokki, Giza 12622, EgyptClinical manifestation of gastric ulcers is frequent, in addition to their costly drug regimens, warranting the development of novel drugs at lower costs. Although <i>Bassia indica</i> is well characterized for its anti-inflammatory and antioxidant potential, capacity of its ethanol extract (BIEE) to prevent stomach ulcers’ progression has not been reported. A nuclear protein termed high-mobility group box 1 (HMGB1) plays a key role in the formation of stomach ulcers by triggering a number of inflammatory responses. The main purpose of the current investigation was to evaluate the in vivo anti-inflammatory and anti-ulcerogenic capabilities of BIEE against ethanol-induced gastric ulcers in rats via the HMGB1/TLR-4/NF-B signaling pathway. HMGB1 and Nuclear factor kappa (NF-B) expression, IL-1β and Nrf2 contents showed an increase along with ulcer development, concurrent with an increase in immunohistochemical TLR-4 level. In contrast, pre-treatment with BIEE significantly reduced HMGB1 and Nuclear factor kappa (NF-B) expression levels, IL-1β and Nrf2 contents and ulcer index value. Such protective action was further confirmed based on histological and immunohistochemical TLR-4 assays. Untargeted analysis via UPLC-ESI–Qtof-MS has allowed for the comprehensive characterization of 40 metabolites in BIEE mostly belonged to two main chemical classes, <i>viz</i>., flavonoids and lipids. These key metabolites, particularly flavonoids, suggesting a mediation for the anti-inflammatory and anti-ulcerogenic properties of BIEE, pose it as a promising natural drug regimen for treatment of stomach ulcers.https://www.mdpi.com/2076-3921/12/6/1263gastroprotection<i>Bassia indica</i>HMGB1NF-κBTLR-4IL-1β |
| spellingShingle | Zeinab A. El-Gendy Rehab F. Taher Abdelbaset M. Elgamal Ahmed Serag Azza Hassan Gehad A. Abdel Jaleel Mohamed A. Farag Abdelsamed I. Elshamy Metabolites Profiling and Bioassays Reveal <i>Bassia indica</i> Ethanol Extract Protective Effect against Stomach Ulcers Development via HMGB1/TLR-4/NF-κB Pathway Antioxidants gastroprotection <i>Bassia indica</i> HMGB1 NF-κB TLR-4 IL-1β |
| title | Metabolites Profiling and Bioassays Reveal <i>Bassia indica</i> Ethanol Extract Protective Effect against Stomach Ulcers Development via HMGB1/TLR-4/NF-κB Pathway |
| title_full | Metabolites Profiling and Bioassays Reveal <i>Bassia indica</i> Ethanol Extract Protective Effect against Stomach Ulcers Development via HMGB1/TLR-4/NF-κB Pathway |
| title_fullStr | Metabolites Profiling and Bioassays Reveal <i>Bassia indica</i> Ethanol Extract Protective Effect against Stomach Ulcers Development via HMGB1/TLR-4/NF-κB Pathway |
| title_full_unstemmed | Metabolites Profiling and Bioassays Reveal <i>Bassia indica</i> Ethanol Extract Protective Effect against Stomach Ulcers Development via HMGB1/TLR-4/NF-κB Pathway |
| title_short | Metabolites Profiling and Bioassays Reveal <i>Bassia indica</i> Ethanol Extract Protective Effect against Stomach Ulcers Development via HMGB1/TLR-4/NF-κB Pathway |
| title_sort | metabolites profiling and bioassays reveal i bassia indica i ethanol extract protective effect against stomach ulcers development via hmgb1 tlr 4 nf κb pathway |
| topic | gastroprotection <i>Bassia indica</i> HMGB1 NF-κB TLR-4 IL-1β |
| url | https://www.mdpi.com/2076-3921/12/6/1263 |
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