| Summary: | This study aimed to determine the effects of bovine amniotic fluid combined with bone
graft in treating peri-implant bone defects with guided bone regeneration. Twenty female
Sprague–Dawley rats were divided into two groups. Bone sockets with a diameter of 4
mm in the coronal part and a diameter of 2.5 mm in the apical part of the implant were
created into the corticocancellous bone in the metaphyseal parts of the right tibia bones
of all subjects. Implants with a length of 4 mm and a diameter of 2.5 mm were placed in
the bone sockets. In the sham surgery group (n = 10) was the circumferential bone defect
equivalent to half of the 4-mm implant length, which occurred between the implant
and the bone, filled with bovine xenograft. Bovine xenografts were filled with amniotic
fluid mixture in the experimental group (n = 10). After 8 weeks of recovery, all rats were
sacrificed. The implants were extracted from the soft tissues and the surrounding bone.
Subsequently, the bones were decalcified and prepared for histological analysis. The
percentage of newly regenerated bone (NRB) formation and fibrosis in the bone defect
area around the implant was calculated from all sections. NRB was found in 37.4±4.4%
of controls and 41.4±2.63% of test animals (P<0.05 and P=0.024, respectively). Fibrosis
formation was found at a rate of 38.6±5.06% in the control group and 33.2±5.38% in the
test group (P<0.05 and P=0.033, respectively). It was considered that combining bovine
amniotic fluid with bone transplant could be a useful way of treating bone abnormalities.
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