A Community-Based Culture Collection for Targeting Novel Plant Growth-Promoting Bacteria from the Sugarcane Microbiome
The soil-plant ecosystem harbors an immense microbial diversity that challenges investigative approaches to study traits underlying plant-microbe association. Studies solely based on culture-dependent techniques have overlooked most microbial diversity. Here we describe the concomitant use of cultur...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-01-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2017.02191/full |
| _version_ | 1818060547925475328 |
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| author | Jaderson Silveira Leite Armanhi Jaderson Silveira Leite Armanhi Rafael Soares Correa de Souza Rafael Soares Correa de Souza Natália de Brito Damasceno Natália de Brito Damasceno Laura M. de Araújo Laura M. de Araújo Juan Imperial Juan Imperial Paulo Arruda Paulo Arruda |
| author_facet | Jaderson Silveira Leite Armanhi Jaderson Silveira Leite Armanhi Rafael Soares Correa de Souza Rafael Soares Correa de Souza Natália de Brito Damasceno Natália de Brito Damasceno Laura M. de Araújo Laura M. de Araújo Juan Imperial Juan Imperial Paulo Arruda Paulo Arruda |
| author_sort | Jaderson Silveira Leite Armanhi |
| collection | DOAJ |
| description | The soil-plant ecosystem harbors an immense microbial diversity that challenges investigative approaches to study traits underlying plant-microbe association. Studies solely based on culture-dependent techniques have overlooked most microbial diversity. Here we describe the concomitant use of culture-dependent and -independent techniques to target plant-beneficial microbial groups from the sugarcane microbiome. The community-based culture collection (CBC) approach was used to access microbes from roots and stalks. The CBC recovered 399 unique bacteria representing 15.9% of the rhizosphere core microbiome and 61.6–65.3% of the endophytic core microbiomes of stalks. By cross-referencing the CBC (culture-dependent) with the sugarcane microbiome profile (culture-independent), we designed a synthetic community comprised of naturally occurring highly abundant bacterial groups from roots and stalks, most of which has been poorly explored so far. We then used maize as a model to probe the abundance-based synthetic inoculant. We show that when inoculated in maize plants, members of the synthetic community efficiently colonize plant organs, displace the natural microbiota and dominate at 53.9% of the rhizosphere microbial abundance. As a result, inoculated plants increased biomass by 3.4-fold as compared to uninoculated plants. The results demonstrate that abundance-based synthetic inoculants can be successfully applied to recover beneficial plant microbes from plant microbiota. |
| first_indexed | 2024-12-10T13:34:10Z |
| format | Article |
| id | doaj.art-0e034adbd5174155b0d71f52b26eda21 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-10T13:34:10Z |
| publishDate | 2018-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-0e034adbd5174155b0d71f52b26eda212022-12-22T01:46:52ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2018-01-01810.3389/fpls.2017.02191317588A Community-Based Culture Collection for Targeting Novel Plant Growth-Promoting Bacteria from the Sugarcane MicrobiomeJaderson Silveira Leite Armanhi0Jaderson Silveira Leite Armanhi1Rafael Soares Correa de Souza2Rafael Soares Correa de Souza3Natália de Brito Damasceno4Natália de Brito Damasceno5Laura M. de Araújo6Laura M. de Araújo7Juan Imperial8Juan Imperial9Paulo Arruda10Paulo Arruda11Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Campinas, BrazilDepartamento de Genética e Evolução, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, BrazilCentro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Campinas, BrazilDepartamento de Genética e Evolução, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, BrazilCentro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Campinas, BrazilDepartamento de Genética e Evolução, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, BrazilCentro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Campinas, BrazilDepartamento de Genética e Evolução, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, BrazilCentro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid, SpainConsejo Superior de Investigaciones Científicas, Madrid, SpainCentro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Campinas, BrazilDepartamento de Genética e Evolução, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, BrazilThe soil-plant ecosystem harbors an immense microbial diversity that challenges investigative approaches to study traits underlying plant-microbe association. Studies solely based on culture-dependent techniques have overlooked most microbial diversity. Here we describe the concomitant use of culture-dependent and -independent techniques to target plant-beneficial microbial groups from the sugarcane microbiome. The community-based culture collection (CBC) approach was used to access microbes from roots and stalks. The CBC recovered 399 unique bacteria representing 15.9% of the rhizosphere core microbiome and 61.6–65.3% of the endophytic core microbiomes of stalks. By cross-referencing the CBC (culture-dependent) with the sugarcane microbiome profile (culture-independent), we designed a synthetic community comprised of naturally occurring highly abundant bacterial groups from roots and stalks, most of which has been poorly explored so far. We then used maize as a model to probe the abundance-based synthetic inoculant. We show that when inoculated in maize plants, members of the synthetic community efficiently colonize plant organs, displace the natural microbiota and dominate at 53.9% of the rhizosphere microbial abundance. As a result, inoculated plants increased biomass by 3.4-fold as compared to uninoculated plants. The results demonstrate that abundance-based synthetic inoculants can be successfully applied to recover beneficial plant microbes from plant microbiota.http://journal.frontiersin.org/article/10.3389/fpls.2017.02191/fullcommunity-based culture collection (CBC)maizemicrobiomeplant growth-promoting (PGP)sugarcanesynthetic community |
| spellingShingle | Jaderson Silveira Leite Armanhi Jaderson Silveira Leite Armanhi Rafael Soares Correa de Souza Rafael Soares Correa de Souza Natália de Brito Damasceno Natália de Brito Damasceno Laura M. de Araújo Laura M. de Araújo Juan Imperial Juan Imperial Paulo Arruda Paulo Arruda A Community-Based Culture Collection for Targeting Novel Plant Growth-Promoting Bacteria from the Sugarcane Microbiome Frontiers in Plant Science community-based culture collection (CBC) maize microbiome plant growth-promoting (PGP) sugarcane synthetic community |
| title | A Community-Based Culture Collection for Targeting Novel Plant Growth-Promoting Bacteria from the Sugarcane Microbiome |
| title_full | A Community-Based Culture Collection for Targeting Novel Plant Growth-Promoting Bacteria from the Sugarcane Microbiome |
| title_fullStr | A Community-Based Culture Collection for Targeting Novel Plant Growth-Promoting Bacteria from the Sugarcane Microbiome |
| title_full_unstemmed | A Community-Based Culture Collection for Targeting Novel Plant Growth-Promoting Bacteria from the Sugarcane Microbiome |
| title_short | A Community-Based Culture Collection for Targeting Novel Plant Growth-Promoting Bacteria from the Sugarcane Microbiome |
| title_sort | community based culture collection for targeting novel plant growth promoting bacteria from the sugarcane microbiome |
| topic | community-based culture collection (CBC) maize microbiome plant growth-promoting (PGP) sugarcane synthetic community |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2017.02191/full |
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