Juvenile depletion of microglia reduces orientation but not high spatial frequency selectivity in mouse V1
Abstract Microglia contain multiple mechanisms that shape the synaptic landscape during postnatal development. Whether the synaptic changes mediated by microglia reflect the developmental refinement of neuronal responses in sensory cortices, however, remains poorly understood. In postnatal life, the...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-15503-0 |
| _version_ | 1818162971669430272 |
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| author | Dario X. Figueroa Velez Miguel Arreola Carey Y. L. Huh Kim Green Sunil P. Gandhi |
| author_facet | Dario X. Figueroa Velez Miguel Arreola Carey Y. L. Huh Kim Green Sunil P. Gandhi |
| author_sort | Dario X. Figueroa Velez |
| collection | DOAJ |
| description | Abstract Microglia contain multiple mechanisms that shape the synaptic landscape during postnatal development. Whether the synaptic changes mediated by microglia reflect the developmental refinement of neuronal responses in sensory cortices, however, remains poorly understood. In postnatal life, the development of increased orientation and spatial frequency selectivity of neuronal responses in primary visual cortex (V1) supports the emergence of high visual acuity. Here, we used the colony-stimulating factor 1 receptor (CSF1R) inhibitor PLX5622 to rapidly and durably deplete microglia in mice during the juvenile period in which increased orientation and spatial frequency selectivity emerge. Excitatory and inhibitory tuning properties were measured simultaneously using multi-photon calcium imaging in layer II/III of mouse V1. We found that microglia depletion generally increased evoked activity which, in turn, reduced orientation selectivity. Surprisingly, microglia were not required for the emergence of high spatial frequency tuned responses. In addition, microglia depletion did not perturb cortical binocularity, suggesting normal depth processing. Together, our finding that orientation and high spatial frequency selectivity in V1 are differentially supported by microglia reveal that microglia are required normal sensory processing, albeit selectively. |
| first_indexed | 2024-12-11T16:42:09Z |
| format | Article |
| id | doaj.art-0e050df615c24f5285908d2097c9b7c7 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-11T16:42:09Z |
| publishDate | 2022-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-0e050df615c24f5285908d2097c9b7c72022-12-22T00:58:17ZengNature PortfolioScientific Reports2045-23222022-07-0112111210.1038/s41598-022-15503-0Juvenile depletion of microglia reduces orientation but not high spatial frequency selectivity in mouse V1Dario X. Figueroa Velez0Miguel Arreola1Carey Y. L. Huh2Kim Green3Sunil P. Gandhi4Department of Pathology, Children’s Hospital BostonDepartment of Neurobiology and Behavior, University of CaliforniaDepartment of Neurobiology and Behavior, University of CaliforniaDepartment of Neurobiology and Behavior, University of CaliforniaDepartment of Neurobiology and Behavior, University of CaliforniaAbstract Microglia contain multiple mechanisms that shape the synaptic landscape during postnatal development. Whether the synaptic changes mediated by microglia reflect the developmental refinement of neuronal responses in sensory cortices, however, remains poorly understood. In postnatal life, the development of increased orientation and spatial frequency selectivity of neuronal responses in primary visual cortex (V1) supports the emergence of high visual acuity. Here, we used the colony-stimulating factor 1 receptor (CSF1R) inhibitor PLX5622 to rapidly and durably deplete microglia in mice during the juvenile period in which increased orientation and spatial frequency selectivity emerge. Excitatory and inhibitory tuning properties were measured simultaneously using multi-photon calcium imaging in layer II/III of mouse V1. We found that microglia depletion generally increased evoked activity which, in turn, reduced orientation selectivity. Surprisingly, microglia were not required for the emergence of high spatial frequency tuned responses. In addition, microglia depletion did not perturb cortical binocularity, suggesting normal depth processing. Together, our finding that orientation and high spatial frequency selectivity in V1 are differentially supported by microglia reveal that microglia are required normal sensory processing, albeit selectively.https://doi.org/10.1038/s41598-022-15503-0 |
| spellingShingle | Dario X. Figueroa Velez Miguel Arreola Carey Y. L. Huh Kim Green Sunil P. Gandhi Juvenile depletion of microglia reduces orientation but not high spatial frequency selectivity in mouse V1 Scientific Reports |
| title | Juvenile depletion of microglia reduces orientation but not high spatial frequency selectivity in mouse V1 |
| title_full | Juvenile depletion of microglia reduces orientation but not high spatial frequency selectivity in mouse V1 |
| title_fullStr | Juvenile depletion of microglia reduces orientation but not high spatial frequency selectivity in mouse V1 |
| title_full_unstemmed | Juvenile depletion of microglia reduces orientation but not high spatial frequency selectivity in mouse V1 |
| title_short | Juvenile depletion of microglia reduces orientation but not high spatial frequency selectivity in mouse V1 |
| title_sort | juvenile depletion of microglia reduces orientation but not high spatial frequency selectivity in mouse v1 |
| url | https://doi.org/10.1038/s41598-022-15503-0 |
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