Gut microbiome and serum amino acid metabolome alterations in autism spectrum disorder
Abstract Gut microbiota and their metabolic products might play important roles in regulating the pathogenesis of autism spectrum disorder (ASD). The purpose of this study was to characterize gut microbiota and serum amino acid metabolome profiles in children with ASD. A non-randomized controlled st...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-02-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-54717-2 |
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| author | Xuening Chang Yuchen Zhang Xue Chen Shihan Li Hong Mei Han Xiao Xinyu Ma Zhisheng Liu Ruizhen Li |
| author_facet | Xuening Chang Yuchen Zhang Xue Chen Shihan Li Hong Mei Han Xiao Xinyu Ma Zhisheng Liu Ruizhen Li |
| author_sort | Xuening Chang |
| collection | DOAJ |
| description | Abstract Gut microbiota and their metabolic products might play important roles in regulating the pathogenesis of autism spectrum disorder (ASD). The purpose of this study was to characterize gut microbiota and serum amino acid metabolome profiles in children with ASD. A non-randomized controlled study was carried out to analyze the alterations in the intestinal microbiota and their metabolites in patients with ASD (n = 30) compared with neurotypical controls (NC) (n = 30) by metagenomic sequencing to define the gut microbiota community and liquid chromatography/mass spectrometry (LC/MS) analysis to characterize the metabolite profiles. Compared with children in the NC group, those in the ASD group showed lower richness, higher evenness, and an altered microbial community structure. At the class level, Deinococci and Holophagae were significantly lower in children with ASD compared with TD. At the phylum level, Deinococcus-Thermus was significantly lower in children with ASD compared with TD. In addition, the functional properties (such as galactose metabolism) displayed significant differences between the ASD and NC groups. Five dominant altered species were identified and analyzed (LDA score > 2.0, P < 0.05), including Subdoligranulum, Faecalibacterium_praushitzii, Faecalibacterium, Veillonellaceae, and Rumminococcaceae. The peptides/nickel transport system was the main metabolic pathway involved in the differential species in the ASD group. Decreased ornithine levels and elevated valine levels may increase the risk of ASD through a metabolic pathway known as the nickel transport system. The microbial metabolism in diverse environments was negatively correlated with phascolarctobacterium succinatutens. Our study provides novel insights into compositional and functional alterations in the gut microbiome and metabolite profiles in ASD and the underlying mechanisms between metabolite and ASD. |
| first_indexed | 2024-03-07T15:06:51Z |
| format | Article |
| id | doaj.art-0e06edbb6de345a6bcce5bec5098564b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-07T15:06:51Z |
| publishDate | 2024-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-0e06edbb6de345a6bcce5bec5098564b2024-03-05T18:51:51ZengNature PortfolioScientific Reports2045-23222024-02-0114111910.1038/s41598-024-54717-2Gut microbiome and serum amino acid metabolome alterations in autism spectrum disorderXuening Chang0Yuchen Zhang1Xue Chen2Shihan Li3Hong Mei4Han Xiao5Xinyu Ma6Zhisheng Liu7Ruizhen Li8Department of Child Health Care, Wuhan Children’s Hospital, Tongji Medical College, Huazhong University of Science and TechnologySchool of Medicine, Wuhan University of Science and TechnologySchool of Medicine, Wuhan University of Science and TechnologyDepartment of Child Health Care, Wuhan Children’s Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Maternal and Child Health, Wuhan Children’s Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Maternal and Child Health, Wuhan Children’s Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Radiology, Wuhan Children’s Hospital, Tongji Medical College, Huazhong University of Science and TechnologyWuhan Children’s Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Child Health Care, Wuhan Children’s Hospital, Tongji Medical College, Huazhong University of Science and TechnologyAbstract Gut microbiota and their metabolic products might play important roles in regulating the pathogenesis of autism spectrum disorder (ASD). The purpose of this study was to characterize gut microbiota and serum amino acid metabolome profiles in children with ASD. A non-randomized controlled study was carried out to analyze the alterations in the intestinal microbiota and their metabolites in patients with ASD (n = 30) compared with neurotypical controls (NC) (n = 30) by metagenomic sequencing to define the gut microbiota community and liquid chromatography/mass spectrometry (LC/MS) analysis to characterize the metabolite profiles. Compared with children in the NC group, those in the ASD group showed lower richness, higher evenness, and an altered microbial community structure. At the class level, Deinococci and Holophagae were significantly lower in children with ASD compared with TD. At the phylum level, Deinococcus-Thermus was significantly lower in children with ASD compared with TD. In addition, the functional properties (such as galactose metabolism) displayed significant differences between the ASD and NC groups. Five dominant altered species were identified and analyzed (LDA score > 2.0, P < 0.05), including Subdoligranulum, Faecalibacterium_praushitzii, Faecalibacterium, Veillonellaceae, and Rumminococcaceae. The peptides/nickel transport system was the main metabolic pathway involved in the differential species in the ASD group. Decreased ornithine levels and elevated valine levels may increase the risk of ASD through a metabolic pathway known as the nickel transport system. The microbial metabolism in diverse environments was negatively correlated with phascolarctobacterium succinatutens. Our study provides novel insights into compositional and functional alterations in the gut microbiome and metabolite profiles in ASD and the underlying mechanisms between metabolite and ASD.https://doi.org/10.1038/s41598-024-54717-2ASDGut microbiotaMetagenomicsMetabolism |
| spellingShingle | Xuening Chang Yuchen Zhang Xue Chen Shihan Li Hong Mei Han Xiao Xinyu Ma Zhisheng Liu Ruizhen Li Gut microbiome and serum amino acid metabolome alterations in autism spectrum disorder Scientific Reports ASD Gut microbiota Metagenomics Metabolism |
| title | Gut microbiome and serum amino acid metabolome alterations in autism spectrum disorder |
| title_full | Gut microbiome and serum amino acid metabolome alterations in autism spectrum disorder |
| title_fullStr | Gut microbiome and serum amino acid metabolome alterations in autism spectrum disorder |
| title_full_unstemmed | Gut microbiome and serum amino acid metabolome alterations in autism spectrum disorder |
| title_short | Gut microbiome and serum amino acid metabolome alterations in autism spectrum disorder |
| title_sort | gut microbiome and serum amino acid metabolome alterations in autism spectrum disorder |
| topic | ASD Gut microbiota Metagenomics Metabolism |
| url | https://doi.org/10.1038/s41598-024-54717-2 |
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