Involvement of Mycoplasma synoviae in Respiratory Distress Cases of Broilers
Mycoplasma synoviae (MS) is an important pathogen of poultry worldwide, causing respiratory tract infection and infectious synovitis in chickens and turkeys. The study was designed to detect M. synoviae through serology, culture isolation and polymerase chain reaction (PCR) assay to document the inv...
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| Format: | Article |
| Language: | English |
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University of Agriculture, Faisalabad
2011-04-01
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| Series: | Pakistan Veterinary Journal |
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| Online Access: | http://www.pvj.com.pk/pdf-files/31_2/117-119.pdf |
| _version_ | 1811232362635198464 |
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| author | S. Ehtisham-ul-Haque*, S. U. Rahman, M. Siddique and A. S. Qureshi1 |
| author_facet | S. Ehtisham-ul-Haque*, S. U. Rahman, M. Siddique and A. S. Qureshi1 |
| author_sort | S. Ehtisham-ul-Haque*, S. U. Rahman, M. Siddique and A. S. Qureshi1 |
| collection | DOAJ |
| description | Mycoplasma synoviae (MS) is an important pathogen of poultry worldwide, causing respiratory tract infection and infectious synovitis in chickens and turkeys. The study was designed to detect M. synoviae through serology, culture isolation and polymerase chain reaction (PCR) assay to document the involvement of MS infection in respiratory distress cases of broiler birds. The validated PCR assay amplifying the conserved gene region of 16SrRNA gene was applied for the detection of M. synoviae from culture as well as in clinical samples. The results indicated that 04 out of total 17 commercial broiler flocks showing respiratory distress signs were found positive with M. synoviae infection indicating 76.57% sero-positivity as, determined with rapid serum agglutination (RSA) test. Out of 85 clinical specimens (collected from sero-positive birds); M. synoviae culture isolation was successfully attained in 36 (42.35%) samples. Whereas, PCR test has detected 84 (98.82%) positive cases. The prevalence of MS in broiler birds was observed maximum as measured through PCR. It is suggested that the true prevalence of MS may best be reflected by combining RSA and PCR test findings. |
| first_indexed | 2024-04-12T11:01:57Z |
| format | Article |
| id | doaj.art-0e0736f747d24377a54afbd1732cd8aa |
| institution | Directory Open Access Journal |
| issn | 0253-8318 |
| language | English |
| last_indexed | 2024-04-12T11:01:57Z |
| publishDate | 2011-04-01 |
| publisher | University of Agriculture, Faisalabad |
| record_format | Article |
| series | Pakistan Veterinary Journal |
| spelling | doaj.art-0e0736f747d24377a54afbd1732cd8aa2022-12-22T03:35:56ZengUniversity of Agriculture, FaisalabadPakistan Veterinary Journal0253-83182011-04-01312117119Involvement of Mycoplasma synoviae in Respiratory Distress Cases of BroilersS. Ehtisham-ul-Haque*, S. U. Rahman, M. Siddique and A. S. Qureshi1Mycoplasma synoviae (MS) is an important pathogen of poultry worldwide, causing respiratory tract infection and infectious synovitis in chickens and turkeys. The study was designed to detect M. synoviae through serology, culture isolation and polymerase chain reaction (PCR) assay to document the involvement of MS infection in respiratory distress cases of broiler birds. The validated PCR assay amplifying the conserved gene region of 16SrRNA gene was applied for the detection of M. synoviae from culture as well as in clinical samples. The results indicated that 04 out of total 17 commercial broiler flocks showing respiratory distress signs were found positive with M. synoviae infection indicating 76.57% sero-positivity as, determined with rapid serum agglutination (RSA) test. Out of 85 clinical specimens (collected from sero-positive birds); M. synoviae culture isolation was successfully attained in 36 (42.35%) samples. Whereas, PCR test has detected 84 (98.82%) positive cases. The prevalence of MS in broiler birds was observed maximum as measured through PCR. It is suggested that the true prevalence of MS may best be reflected by combining RSA and PCR test findings.http://www.pvj.com.pk/pdf-files/31_2/117-119.pdfBroilersM. synoviaePCRRapid Serum Agglutination16SrRNA gene |
| spellingShingle | S. Ehtisham-ul-Haque*, S. U. Rahman, M. Siddique and A. S. Qureshi1 Involvement of Mycoplasma synoviae in Respiratory Distress Cases of Broilers Pakistan Veterinary Journal Broilers M. synoviae PCR Rapid Serum Agglutination 16SrRNA gene |
| title | Involvement of Mycoplasma synoviae in Respiratory Distress Cases of Broilers |
| title_full | Involvement of Mycoplasma synoviae in Respiratory Distress Cases of Broilers |
| title_fullStr | Involvement of Mycoplasma synoviae in Respiratory Distress Cases of Broilers |
| title_full_unstemmed | Involvement of Mycoplasma synoviae in Respiratory Distress Cases of Broilers |
| title_short | Involvement of Mycoplasma synoviae in Respiratory Distress Cases of Broilers |
| title_sort | involvement of mycoplasma synoviae in respiratory distress cases of broilers |
| topic | Broilers M. synoviae PCR Rapid Serum Agglutination 16SrRNA gene |
| url | http://www.pvj.com.pk/pdf-files/31_2/117-119.pdf |
| work_keys_str_mv | AT sehtishamulhaquesurahmanmsiddiqueandasqureshi1 involvementofmycoplasmasynoviaeinrespiratorydistresscasesofbroilers |