Screening of differentially expressed microRNAs of essential hypertension in Uyghur population
Abstract Background Essential hypertension can cause many kinds of cardiovascular diseases. The pathogenesis of essential hypertension is very complex, and the mechanism is still unclear. The microRNAs have been identified as novel biomarkers for pre-diagnosis and prognosis of hypertension. However,...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-04-01
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| Series: | Lipids in Health and Disease |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12944-019-1028-1 |
| _version_ | 1819162158985904128 |
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| author | Yuanzheng Ye Jianzhong Yang Wenkui Lv Yanmei Lu Ling Zhang Ying Zhang Zulifeiya Musha Ping Fan Bin Yang Xianhui Zhou Baopeng Tang |
| author_facet | Yuanzheng Ye Jianzhong Yang Wenkui Lv Yanmei Lu Ling Zhang Ying Zhang Zulifeiya Musha Ping Fan Bin Yang Xianhui Zhou Baopeng Tang |
| author_sort | Yuanzheng Ye |
| collection | DOAJ |
| description | Abstract Background Essential hypertension can cause many kinds of cardiovascular diseases. The pathogenesis of essential hypertension is very complex, and the mechanism is still unclear. The microRNAs have been identified as novel biomarkers for pre-diagnosis and prognosis of hypertension. However, the kinds of microRNAs that can be used as specific biomarkers for hypertension are unknown. Methods and results Plasma samples were isolated from Uyghur subjects with essential hypertension and the healthy individuals. Microarray was used to identify differentially expressed microRNAs. The microarray data were clustered and annotated with online software. The target genes of differentially expressed microRNAs were also analyzed. The microarray results were further verified by quantitative real-time PCR. We identified 257 microRNAs that were differentially expressed between patients with essential hypertension and the healthy individuals. These microRNAs had a total of 6580 target genes. The 47 microRNAs that had target genes, including 24 up-regulated and 23 down-regulated microRNAs, were further screened out to construct a reference set of potential microRNA biomarkers. Most of the 47 microRNAs were located at chromosome 19 (40 microRNAs) and chromosome 1 (45 microRNAs). Their target genes were mainly enriched in metal ion binding, transcription regulation, cell adhesion and junction, indicating that these candidate microRNAs may regulate mineral ion binding and cell communication process of essential hypertension. The quantitative real-time PCR results of miR-198 and miR-1183 (which were the two most significantly up-regulated microRNAs by microarray), and, miR-30e-5p and miR-144-3p (which were the two most significantly down-regulated microRNAs by microarray) were consistent with the microarray results. Conclusions A reference set of potential microRNA biomarkers that may be involved in essential hypertension is constructed. Our study may provide experimental evidence for further studying the mechanism of essential hypertension. |
| first_indexed | 2024-12-22T17:23:48Z |
| format | Article |
| id | doaj.art-0e08c66ce39242e883af1a56e99adc17 |
| institution | Directory Open Access Journal |
| issn | 1476-511X |
| language | English |
| last_indexed | 2024-12-22T17:23:48Z |
| publishDate | 2019-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Lipids in Health and Disease |
| spelling | doaj.art-0e08c66ce39242e883af1a56e99adc172022-12-21T18:18:46ZengBMCLipids in Health and Disease1476-511X2019-04-0118111310.1186/s12944-019-1028-1Screening of differentially expressed microRNAs of essential hypertension in Uyghur populationYuanzheng Ye0Jianzhong Yang1Wenkui Lv2Yanmei Lu3Ling Zhang4Ying Zhang5Zulifeiya Musha6Ping Fan7Bin Yang8Xianhui Zhou9Baopeng Tang10Heart Center, the First Affiliated Hospital of Xinjiang Medical UniversityHeart Center, the First Affiliated Hospital of Xinjiang Medical UniversityHeart Center, the First Affiliated Hospital of Xinjiang Medical UniversityHeart Center, the First Affiliated Hospital of Xinjiang Medical UniversityHeart Center, the First Affiliated Hospital of Xinjiang Medical UniversityHeart Center, the First Affiliated Hospital of Xinjiang Medical UniversityHeart Center, the First Affiliated Hospital of Xinjiang Medical UniversityHeart Center, the First Affiliated Hospital of Xinjiang Medical UniversityHeart Center, the First Affiliated Hospital of Xinjiang Medical UniversityHeart Center, the First Affiliated Hospital of Xinjiang Medical UniversityHeart Center, the First Affiliated Hospital of Xinjiang Medical UniversityAbstract Background Essential hypertension can cause many kinds of cardiovascular diseases. The pathogenesis of essential hypertension is very complex, and the mechanism is still unclear. The microRNAs have been identified as novel biomarkers for pre-diagnosis and prognosis of hypertension. However, the kinds of microRNAs that can be used as specific biomarkers for hypertension are unknown. Methods and results Plasma samples were isolated from Uyghur subjects with essential hypertension and the healthy individuals. Microarray was used to identify differentially expressed microRNAs. The microarray data were clustered and annotated with online software. The target genes of differentially expressed microRNAs were also analyzed. The microarray results were further verified by quantitative real-time PCR. We identified 257 microRNAs that were differentially expressed between patients with essential hypertension and the healthy individuals. These microRNAs had a total of 6580 target genes. The 47 microRNAs that had target genes, including 24 up-regulated and 23 down-regulated microRNAs, were further screened out to construct a reference set of potential microRNA biomarkers. Most of the 47 microRNAs were located at chromosome 19 (40 microRNAs) and chromosome 1 (45 microRNAs). Their target genes were mainly enriched in metal ion binding, transcription regulation, cell adhesion and junction, indicating that these candidate microRNAs may regulate mineral ion binding and cell communication process of essential hypertension. The quantitative real-time PCR results of miR-198 and miR-1183 (which were the two most significantly up-regulated microRNAs by microarray), and, miR-30e-5p and miR-144-3p (which were the two most significantly down-regulated microRNAs by microarray) were consistent with the microarray results. Conclusions A reference set of potential microRNA biomarkers that may be involved in essential hypertension is constructed. Our study may provide experimental evidence for further studying the mechanism of essential hypertension.http://link.springer.com/article/10.1186/s12944-019-1028-1Essential hypertensionUyghur populationmicroRNABiomarker |
| spellingShingle | Yuanzheng Ye Jianzhong Yang Wenkui Lv Yanmei Lu Ling Zhang Ying Zhang Zulifeiya Musha Ping Fan Bin Yang Xianhui Zhou Baopeng Tang Screening of differentially expressed microRNAs of essential hypertension in Uyghur population Lipids in Health and Disease Essential hypertension Uyghur population microRNA Biomarker |
| title | Screening of differentially expressed microRNAs of essential hypertension in Uyghur population |
| title_full | Screening of differentially expressed microRNAs of essential hypertension in Uyghur population |
| title_fullStr | Screening of differentially expressed microRNAs of essential hypertension in Uyghur population |
| title_full_unstemmed | Screening of differentially expressed microRNAs of essential hypertension in Uyghur population |
| title_short | Screening of differentially expressed microRNAs of essential hypertension in Uyghur population |
| title_sort | screening of differentially expressed micrornas of essential hypertension in uyghur population |
| topic | Essential hypertension Uyghur population microRNA Biomarker |
| url | http://link.springer.com/article/10.1186/s12944-019-1028-1 |
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