Over-expression of CRTH2 indicates eosinophilic inflammation and poor prognosis in recurrent nasal polyps
BackgroundChronic rhinosinusitis with nasal polyps (CRSwNP) is often characterized by recurrent nasal polyp (NP) growth following surgical removal, but the mechanisms are still not clear. This study aimed to investigate the expression of chemoattractant receptor-homologous molecule expressed on Th2...
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.1046426/full |
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| author | Wenhui Chen Wenhui Chen Shaojuan He Xinyu Xie Xiaorong Yang Chen Duan Ping Ye Xuezhong Li Monica G. Lawrence Larry Borish Larry Borish Xin Feng |
| author_facet | Wenhui Chen Wenhui Chen Shaojuan He Xinyu Xie Xiaorong Yang Chen Duan Ping Ye Xuezhong Li Monica G. Lawrence Larry Borish Larry Borish Xin Feng |
| author_sort | Wenhui Chen |
| collection | DOAJ |
| description | BackgroundChronic rhinosinusitis with nasal polyps (CRSwNP) is often characterized by recurrent nasal polyp (NP) growth following surgical removal, but the mechanisms are still not clear. This study aimed to investigate the expression of chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTH2) receptor on NP and the role it plays in eosinophil inflammation and polyp recurrence.MethodsForty-one CRSwNPs patients and seventeen controls were enrolled in this study. mRNA was extracted from nasal tissues and evaluated for expression of CRTH2. Immunofluorescence staining was performed to confirm the distribution and expression of CRTH2 protein. CRTH2 expression on peripheral blood eosinophils was quantified by flow cytometry. The eosinophil count and clinical implications were also evaluated and their correlations with CRTH2 expression were analyzed.ResultsNasal polyps displayed increased expression of CRTH2 in mRNA level compared with control samples, with the highest expression observed in recurrent NP. Immunofluorescence confirmed over-expression of CRTH2 in recurrent NP and this was independent of the concurrent presence of asthma. CRTH2 expression was positively correlated with tissue eosinophil number (Spearman’s ρ=0.69, P<0.001) and the postoperative sino-nasal outcome test-22 (SNOT-22) score (Spearman’s ρ=0.67, P<0.001). Receiver operating characteristic (ROC) curves revealed CRTH2 was more predictive for NP recurrence compared to either eosinophil number and concomitant asthma, with an area under the ROC curve of 0.9107.ConclusionThe over-expression of CRTH2 in recurrent nasal polyps correlates with greater eosinophilic inflammation and poor prognosis which is independent of concomitant asthma. |
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| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-13T09:09:40Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Immunology |
| spelling | doaj.art-0e08f98bfb7c4c2aa7b73f68b68eef0b2022-12-22T02:52:55ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-11-011310.3389/fimmu.2022.10464261046426Over-expression of CRTH2 indicates eosinophilic inflammation and poor prognosis in recurrent nasal polypsWenhui Chen0Wenhui Chen1Shaojuan He2Xinyu Xie3Xiaorong Yang4Chen Duan5Ping Ye6Xuezhong Li7Monica G. Lawrence8Larry Borish9Larry Borish10Xin Feng11Department of Otorhinolaryngology, Qilu Hospital of Shandong University, National Health Commission (NHC) Key Laboratory of Otorhinolaryngology, Shandong University, Jinan, Shandong, ChinaDepartment of Otolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, ChinaDepartment of Otorhinolaryngology, Qilu Hospital of Shandong University, National Health Commission (NHC) Key Laboratory of Otorhinolaryngology, Shandong University, Jinan, Shandong, ChinaDepartment of Otorhinolaryngology, Qilu Hospital of Shandong University, National Health Commission (NHC) Key Laboratory of Otorhinolaryngology, Shandong University, Jinan, Shandong, ChinaClinical Epidemiology Unit, Qilu Hospital of Shandong University, Jinan, Shandong, ChinaDepartment of Otorhinolaryngology, Qilu Hospital of Shandong University, National Health Commission (NHC) Key Laboratory of Otorhinolaryngology, Shandong University, Jinan, Shandong, ChinaDepartment of Otorhinolaryngology, Qilu Hospital of Shandong University, National Health Commission (NHC) Key Laboratory of Otorhinolaryngology, Shandong University, Jinan, Shandong, ChinaDepartment of Otorhinolaryngology, Qilu Hospital of Shandong University, National Health Commission (NHC) Key Laboratory of Otorhinolaryngology, Shandong University, Jinan, Shandong, ChinaDepartment of Medicine, University of Virginia Health System, Charlottesville, VA, United StatesDepartment of Medicine, University of Virginia Health System, Charlottesville, VA, United StatesDepartment of Microbiology, University of Virginia Health System, Charlottesville, VA, United StatesDepartment of Otorhinolaryngology, Qilu Hospital of Shandong University, National Health Commission (NHC) Key Laboratory of Otorhinolaryngology, Shandong University, Jinan, Shandong, ChinaBackgroundChronic rhinosinusitis with nasal polyps (CRSwNP) is often characterized by recurrent nasal polyp (NP) growth following surgical removal, but the mechanisms are still not clear. This study aimed to investigate the expression of chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTH2) receptor on NP and the role it plays in eosinophil inflammation and polyp recurrence.MethodsForty-one CRSwNPs patients and seventeen controls were enrolled in this study. mRNA was extracted from nasal tissues and evaluated for expression of CRTH2. Immunofluorescence staining was performed to confirm the distribution and expression of CRTH2 protein. CRTH2 expression on peripheral blood eosinophils was quantified by flow cytometry. The eosinophil count and clinical implications were also evaluated and their correlations with CRTH2 expression were analyzed.ResultsNasal polyps displayed increased expression of CRTH2 in mRNA level compared with control samples, with the highest expression observed in recurrent NP. Immunofluorescence confirmed over-expression of CRTH2 in recurrent NP and this was independent of the concurrent presence of asthma. CRTH2 expression was positively correlated with tissue eosinophil number (Spearman’s ρ=0.69, P<0.001) and the postoperative sino-nasal outcome test-22 (SNOT-22) score (Spearman’s ρ=0.67, P<0.001). Receiver operating characteristic (ROC) curves revealed CRTH2 was more predictive for NP recurrence compared to either eosinophil number and concomitant asthma, with an area under the ROC curve of 0.9107.ConclusionThe over-expression of CRTH2 in recurrent nasal polyps correlates with greater eosinophilic inflammation and poor prognosis which is independent of concomitant asthma.https://www.frontiersin.org/articles/10.3389/fimmu.2022.1046426/fullchemoattractant receptor-homologous molecule expressed on Th2 cells (CRTh2)chronic rhinosinusitis with nasal polypseosinophilsprognosisrecurrent nasal polyp |
| spellingShingle | Wenhui Chen Wenhui Chen Shaojuan He Xinyu Xie Xiaorong Yang Chen Duan Ping Ye Xuezhong Li Monica G. Lawrence Larry Borish Larry Borish Xin Feng Over-expression of CRTH2 indicates eosinophilic inflammation and poor prognosis in recurrent nasal polyps Frontiers in Immunology chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTh2) chronic rhinosinusitis with nasal polyps eosinophils prognosis recurrent nasal polyp |
| title | Over-expression of CRTH2 indicates eosinophilic inflammation and poor prognosis in recurrent nasal polyps |
| title_full | Over-expression of CRTH2 indicates eosinophilic inflammation and poor prognosis in recurrent nasal polyps |
| title_fullStr | Over-expression of CRTH2 indicates eosinophilic inflammation and poor prognosis in recurrent nasal polyps |
| title_full_unstemmed | Over-expression of CRTH2 indicates eosinophilic inflammation and poor prognosis in recurrent nasal polyps |
| title_short | Over-expression of CRTH2 indicates eosinophilic inflammation and poor prognosis in recurrent nasal polyps |
| title_sort | over expression of crth2 indicates eosinophilic inflammation and poor prognosis in recurrent nasal polyps |
| topic | chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTh2) chronic rhinosinusitis with nasal polyps eosinophils prognosis recurrent nasal polyp |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.1046426/full |
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