Co-infection of sexually transmitted pathogens and Human Papillomavirus in cervical samples of women of Brazil
Abstract Background Some sexually transmitted infectious agents, such as Chlamydia trachomatis and Herpes simplex, cause local inflammation, and could contribute to Human Papillomavirus (HPV) and cervical lesion progression. Thus, the aim of this study was to determine any association between the pr...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2017-12-01
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| Series: | BMC Infectious Diseases |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12879-017-2835-5 |
| _version_ | 1819113960659484672 |
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| author | Aline Teixeira Amorim Lucas Miranda Marques Guilherme Barreto Campos Tássia Neves Lobão Vanesca de Souza Lino Ricardo Cesar Cintra Maria Antonieta Andreoli Luisa Lina Villa Enrique Boccardo Antonio Carlos Ricardo Braga Junior Rossana Verónica Mendoza López Djanilson Barbosa dos Santos Gerson Maciel de Souza Carla Cristina Romano Jorge Timenetsky |
| author_facet | Aline Teixeira Amorim Lucas Miranda Marques Guilherme Barreto Campos Tássia Neves Lobão Vanesca de Souza Lino Ricardo Cesar Cintra Maria Antonieta Andreoli Luisa Lina Villa Enrique Boccardo Antonio Carlos Ricardo Braga Junior Rossana Verónica Mendoza López Djanilson Barbosa dos Santos Gerson Maciel de Souza Carla Cristina Romano Jorge Timenetsky |
| author_sort | Aline Teixeira Amorim |
| collection | DOAJ |
| description | Abstract Background Some sexually transmitted infectious agents, such as Chlamydia trachomatis and Herpes simplex, cause local inflammation, and could contribute to Human Papillomavirus (HPV) and cervical lesion progression. Thus, the aim of this study was to determine any association between the presence of microorganisms of gynecological importance, sexual behavior, clinical and demographical variables to the development and progress of cervical lesions. Methods One hundred and thirty-two women between 14 and 78 years and living at Vitória da Conquista, Bahia, Brazil, were included (62 individuals with cervical lesions and 70 without lesions). They answered a questionnaire to provide data for a socioeconomic and sexual activity profile. Samples of cervical swabs were collected and analyzed by PCR to detect genital microorganisms and HPV. Quantitative PCR was used to detect and quantify Ureaplasma urealyticum and Ureaplasma parvum. Univariate and multiple logistic regression were performed to measure the association with the cervical lesions, and an odds ratio (OR) with 95% confidence intervals (95%CI) were calculated. The Mann-Whitney U test was also used to compare the microorganism load in the case and control groups. The significance level was 5% in all hypotheses tested. Results Cervical lesions were associated with: women in a stable sexual relationship (OR = 14.21, 95%CI = 3.67–55.018), positive PCR for HPV (OR = 16.81, 95%CI = 4.19–67.42), Trichomonas vaginalis (OR = 8.566, 95%CI = 2.04–35.94) and Gardnerella vaginalis (OR = 6.13, 95%CI = 1.53–24.61), adjusted by age and qPCR for U. parvum. U. parvum load showed a statistical difference between the case and control groups (p-value = 0.002). Conclusion Variables such as stable relationship, HPV, T. vaginalis, G. vaginalis were associated with cervical lesions in epidemiological studies. U. parvum load was higher in woman with cervical lesions compared with women without lesions. Additional studies are needed to better understand the role of these factors in cervical lesion development. |
| first_indexed | 2024-12-22T04:37:42Z |
| format | Article |
| id | doaj.art-0e0a6b698a0843428549e517d28f7719 |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-12-22T04:37:42Z |
| publishDate | 2017-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-0e0a6b698a0843428549e517d28f77192022-12-21T18:38:50ZengBMCBMC Infectious Diseases1471-23342017-12-0117111210.1186/s12879-017-2835-5Co-infection of sexually transmitted pathogens and Human Papillomavirus in cervical samples of women of BrazilAline Teixeira Amorim0Lucas Miranda Marques1Guilherme Barreto Campos2Tássia Neves Lobão3Vanesca de Souza Lino4Ricardo Cesar Cintra5Maria Antonieta Andreoli6Luisa Lina Villa7Enrique Boccardo8Antonio Carlos Ricardo Braga Junior9Rossana Verónica Mendoza López10Djanilson Barbosa dos Santos11Gerson Maciel de Souza12Carla Cristina Romano13Jorge Timenetsky14Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, ICB/USPDepartamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, ICB/USPDepartamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, ICB/USPDepartamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, ICB/USPDepartamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, ICB/USPDepartamento de Bioquímica, Universidade de São Paulo, USPFaculdade de Ciências Médicas da Santa Casa de São Paulo, FCMSCSP, A.C. Camargo Cancer CenterFaculdade de Medicina da Universidade de São Paulo, Instituto do Câncer do Estado de São PauloDepartamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, ICB/USPInstituto Multidisciplinar em Saúde/Campus Anísio Teixeira, Universidade Federal da Bahia – IMS/CAT-UFBAInstituto do Câncer do Estado de São PauloUniversidade Federal do Recôncavo da BahiaCentro de Oncologia Dr. Geraldo Mattos de SáUniversidade Estadual de Santa Cruz (UESC)Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, ICB/USPAbstract Background Some sexually transmitted infectious agents, such as Chlamydia trachomatis and Herpes simplex, cause local inflammation, and could contribute to Human Papillomavirus (HPV) and cervical lesion progression. Thus, the aim of this study was to determine any association between the presence of microorganisms of gynecological importance, sexual behavior, clinical and demographical variables to the development and progress of cervical lesions. Methods One hundred and thirty-two women between 14 and 78 years and living at Vitória da Conquista, Bahia, Brazil, were included (62 individuals with cervical lesions and 70 without lesions). They answered a questionnaire to provide data for a socioeconomic and sexual activity profile. Samples of cervical swabs were collected and analyzed by PCR to detect genital microorganisms and HPV. Quantitative PCR was used to detect and quantify Ureaplasma urealyticum and Ureaplasma parvum. Univariate and multiple logistic regression were performed to measure the association with the cervical lesions, and an odds ratio (OR) with 95% confidence intervals (95%CI) were calculated. The Mann-Whitney U test was also used to compare the microorganism load in the case and control groups. The significance level was 5% in all hypotheses tested. Results Cervical lesions were associated with: women in a stable sexual relationship (OR = 14.21, 95%CI = 3.67–55.018), positive PCR for HPV (OR = 16.81, 95%CI = 4.19–67.42), Trichomonas vaginalis (OR = 8.566, 95%CI = 2.04–35.94) and Gardnerella vaginalis (OR = 6.13, 95%CI = 1.53–24.61), adjusted by age and qPCR for U. parvum. U. parvum load showed a statistical difference between the case and control groups (p-value = 0.002). Conclusion Variables such as stable relationship, HPV, T. vaginalis, G. vaginalis were associated with cervical lesions in epidemiological studies. U. parvum load was higher in woman with cervical lesions compared with women without lesions. Additional studies are needed to better understand the role of these factors in cervical lesion development.http://link.springer.com/article/10.1186/s12879-017-2835-5Cervical cancerHPVU. ParvumSexually transmitted infections |
| spellingShingle | Aline Teixeira Amorim Lucas Miranda Marques Guilherme Barreto Campos Tássia Neves Lobão Vanesca de Souza Lino Ricardo Cesar Cintra Maria Antonieta Andreoli Luisa Lina Villa Enrique Boccardo Antonio Carlos Ricardo Braga Junior Rossana Verónica Mendoza López Djanilson Barbosa dos Santos Gerson Maciel de Souza Carla Cristina Romano Jorge Timenetsky Co-infection of sexually transmitted pathogens and Human Papillomavirus in cervical samples of women of Brazil BMC Infectious Diseases Cervical cancer HPV U. Parvum Sexually transmitted infections |
| title | Co-infection of sexually transmitted pathogens and Human Papillomavirus in cervical samples of women of Brazil |
| title_full | Co-infection of sexually transmitted pathogens and Human Papillomavirus in cervical samples of women of Brazil |
| title_fullStr | Co-infection of sexually transmitted pathogens and Human Papillomavirus in cervical samples of women of Brazil |
| title_full_unstemmed | Co-infection of sexually transmitted pathogens and Human Papillomavirus in cervical samples of women of Brazil |
| title_short | Co-infection of sexually transmitted pathogens and Human Papillomavirus in cervical samples of women of Brazil |
| title_sort | co infection of sexually transmitted pathogens and human papillomavirus in cervical samples of women of brazil |
| topic | Cervical cancer HPV U. Parvum Sexually transmitted infections |
| url | http://link.springer.com/article/10.1186/s12879-017-2835-5 |
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