Genotyping of Rotavirus by Using RT-PCR Methods
<div>There is a great diversity of rotavirus genotypes circulating worldwide, with dominant genotypes changing from year to year. Rotavirus genotyping was performed by using reverse transcription PCR with type-specifi c-primers. Since rotavirus is a RNA virus that has high mutation rate, there...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universitas Gadjah Mada, Yogyakarta
2015-11-01
|
| Series: | Indonesian Journal of Biotechnology |
| Online Access: | http://journal.ugm.ac.id/ijbiotech/article/view/7863 |
| _version_ | 1811283179226529792 |
|---|---|
| author | Hera Nirwati Tri Wibawa Abu Tholib Aman Yati Soenarto |
| author_facet | Hera Nirwati Tri Wibawa Abu Tholib Aman Yati Soenarto |
| author_sort | Hera Nirwati |
| collection | DOAJ |
| description | <div>There is a great diversity of rotavirus genotypes circulating worldwide, with dominant genotypes changing from year to year. Rotavirus genotyping was performed by using reverse transcription PCR with type-specifi c-primers. Since rotavirus is a RNA virus that has high mutation rate, there was a possibility of technical diffi culty in genotyping due to mutation in the primer binding sites. During Indonesian rotavirus surveillance study 2006-2009, it was reported that 17% of samples subjected for G type and 21% of samples</div><div>subjected for P type were untypeable. The objective of this study was to identify genotypes of the samples that were untypeable previously using RT-PCR based on the method described by Das et al. (1994) and Gentsch et al. (1992). There were 30 samples subjected to G type and 61 samples subjected to P type to be re-typed using method described by Gouvea et al. (1990) and Simmond et al. (2008) for G and P typing, respectively. By using another set of primer, the genotype of all samples was identifi ed. This study highlights the importance of a constant reconsideration of primer sequences employed for the molecular typing of rotaviruses.</div><div><br /></div><div>Key words: rotavirus, G typing, P typing</div> |
| first_indexed | 2024-04-13T02:06:49Z |
| format | Article |
| id | doaj.art-0e0d7c649e524c0980ab905561b48071 |
| institution | Directory Open Access Journal |
| issn | 0853-8654 2089-2241 |
| language | English |
| last_indexed | 2024-04-13T02:06:49Z |
| publishDate | 2015-11-01 |
| publisher | Universitas Gadjah Mada, Yogyakarta |
| record_format | Article |
| series | Indonesian Journal of Biotechnology |
| spelling | doaj.art-0e0d7c649e524c0980ab905561b480712022-12-22T03:07:26ZengUniversitas Gadjah Mada, YogyakartaIndonesian Journal of Biotechnology0853-86542089-22412015-11-011816579Genotyping of Rotavirus by Using RT-PCR MethodsHera Nirwati0Tri Wibawa1Abu Tholib Aman2Yati Soenarto3Department of Microbiology, Faculty of Medicine, Universitas Gadjah Mada, YogyakartaDepartment of Microbiology, Faculty of Medicine, Universitas Gadjah Mada, YogyakartaDepartment of Microbiology, Faculty of Medicine, Universitas Gadjah Mada, YogyakartaDepartments of Pediatric, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta Department of Pediatric, Dr.Sardjito General Hospital, Yogyakarta<div>There is a great diversity of rotavirus genotypes circulating worldwide, with dominant genotypes changing from year to year. Rotavirus genotyping was performed by using reverse transcription PCR with type-specifi c-primers. Since rotavirus is a RNA virus that has high mutation rate, there was a possibility of technical diffi culty in genotyping due to mutation in the primer binding sites. During Indonesian rotavirus surveillance study 2006-2009, it was reported that 17% of samples subjected for G type and 21% of samples</div><div>subjected for P type were untypeable. The objective of this study was to identify genotypes of the samples that were untypeable previously using RT-PCR based on the method described by Das et al. (1994) and Gentsch et al. (1992). There were 30 samples subjected to G type and 61 samples subjected to P type to be re-typed using method described by Gouvea et al. (1990) and Simmond et al. (2008) for G and P typing, respectively. By using another set of primer, the genotype of all samples was identifi ed. This study highlights the importance of a constant reconsideration of primer sequences employed for the molecular typing of rotaviruses.</div><div><br /></div><div>Key words: rotavirus, G typing, P typing</div>http://journal.ugm.ac.id/ijbiotech/article/view/7863 |
| spellingShingle | Hera Nirwati Tri Wibawa Abu Tholib Aman Yati Soenarto Genotyping of Rotavirus by Using RT-PCR Methods Indonesian Journal of Biotechnology |
| title | Genotyping of Rotavirus by Using RT-PCR Methods |
| title_full | Genotyping of Rotavirus by Using RT-PCR Methods |
| title_fullStr | Genotyping of Rotavirus by Using RT-PCR Methods |
| title_full_unstemmed | Genotyping of Rotavirus by Using RT-PCR Methods |
| title_short | Genotyping of Rotavirus by Using RT-PCR Methods |
| title_sort | genotyping of rotavirus by using rt pcr methods |
| url | http://journal.ugm.ac.id/ijbiotech/article/view/7863 |
| work_keys_str_mv | AT heranirwati genotypingofrotavirusbyusingrtpcrmethods AT triwibawa genotypingofrotavirusbyusingrtpcrmethods AT abutholibaman genotypingofrotavirusbyusingrtpcrmethods AT yatisoenarto genotypingofrotavirusbyusingrtpcrmethods |