The study of protein recruitment to UV-induced DNA lesions can be distorted by photoconversion of DNA dyes like Hoechst or DAPI [version 1; referees: 1 approved, 2 approved with reservations]
A common approach used to assess DNA repair factor binding in mammalian cells is to induce DNA damage with a UV laser and follow the movement of GFP-tagged proteins to the site of damage. Often these measurements are performed in the presence of the blue DNA intercalating dye Hoechst or DAPI, which...
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| Format: | Artikel |
| Sprache: | English |
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F1000 Research Ltd
2019-01-01
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| Schriftenreihe: | F1000Research |
| Online Zugang: | https://f1000research.com/articles/8-104/v1 |