Characterization and Genomic Analysis of the Naphthalene-Degrading <i>Delftia tsuruhatensis</i> ULwDis3 Isolated from Seawater
Strains of the genus <i>Delftia</i> are poorly studied microorganisms. In this work, the complete genome of the naphthalene-degrading <i>Delftia tsuruhatensis</i> strain ULwDis3 isolated from seawater of the Gulf of Finland of the Baltic Sea was assembled. For the first time,...
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MDPI AG
2023-04-01
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| Series: | Microorganisms |
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| author | Olesya I. Sazonova Anastasia A. Ivanova Yanina A. Delegan Rostislav A. Streletskii Diana D. Vershinina Sergei L. Sokolov Anna A. Vetrova |
| author_facet | Olesya I. Sazonova Anastasia A. Ivanova Yanina A. Delegan Rostislav A. Streletskii Diana D. Vershinina Sergei L. Sokolov Anna A. Vetrova |
| author_sort | Olesya I. Sazonova |
| collection | DOAJ |
| description | Strains of the genus <i>Delftia</i> are poorly studied microorganisms. In this work, the complete genome of the naphthalene-degrading <i>Delftia tsuruhatensis</i> strain ULwDis3 isolated from seawater of the Gulf of Finland of the Baltic Sea was assembled. For the first time, genes encoding naphthalene cleavage pathways via salicylate and gentisate were identified in a strain of the genus <i>Delftia</i>. The genes are part of one operon (<i>nag</i> genes). Three open reading frames (ORFs) were found in the genome of <i>D. tsuruhatensis</i> strain ULwDis3 that encode gentisate 1.2-dioxygenase. One of the ORFs is part of the <i>nag</i> operon. The physiological and biochemical characteristics of the strain ULwDis3 when cultured in mineral medium with naphthalene as the sole source of carbon and energy were also studied. It was found that after 22 h of growth, the strain stopped consuming naphthalene, and at the same time, naphthalene 1.2-dioxygenase and salicylate 5-hydroxylase activities were not detected. Later, a decrease in the number of living cells and the death of the culture were observed. Gentisate 1.2-dioxygenase activity was detected from the time of gentisate formation until culture death. |
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| format | Article |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-11T04:42:11Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-0e1157a466b94ac1a248f2746fdb9a862023-11-17T20:34:53ZengMDPI AGMicroorganisms2076-26072023-04-01114109210.3390/microorganisms11041092Characterization and Genomic Analysis of the Naphthalene-Degrading <i>Delftia tsuruhatensis</i> ULwDis3 Isolated from SeawaterOlesya I. Sazonova0Anastasia A. Ivanova1Yanina A. Delegan2Rostislav A. Streletskii3Diana D. Vershinina4Sergei L. Sokolov5Anna A. Vetrova6Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”, 142290 Pushchino, RussiaFederal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”, 142290 Pushchino, RussiaFederal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”, 142290 Pushchino, RussiaLaboratory of Ecological Soil Science, Faculty of Soil Science, Lomonosov Moscow State University, 119991 Moscow, RussiaFederal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”, 142290 Pushchino, RussiaFederal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”, 142290 Pushchino, RussiaFederal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”, 142290 Pushchino, RussiaStrains of the genus <i>Delftia</i> are poorly studied microorganisms. In this work, the complete genome of the naphthalene-degrading <i>Delftia tsuruhatensis</i> strain ULwDis3 isolated from seawater of the Gulf of Finland of the Baltic Sea was assembled. For the first time, genes encoding naphthalene cleavage pathways via salicylate and gentisate were identified in a strain of the genus <i>Delftia</i>. The genes are part of one operon (<i>nag</i> genes). Three open reading frames (ORFs) were found in the genome of <i>D. tsuruhatensis</i> strain ULwDis3 that encode gentisate 1.2-dioxygenase. One of the ORFs is part of the <i>nag</i> operon. The physiological and biochemical characteristics of the strain ULwDis3 when cultured in mineral medium with naphthalene as the sole source of carbon and energy were also studied. It was found that after 22 h of growth, the strain stopped consuming naphthalene, and at the same time, naphthalene 1.2-dioxygenase and salicylate 5-hydroxylase activities were not detected. Later, a decrease in the number of living cells and the death of the culture were observed. Gentisate 1.2-dioxygenase activity was detected from the time of gentisate formation until culture death.https://www.mdpi.com/2076-2607/11/4/1092<i>Delftia tsuruhatensis</i>genomenaphthalene biodegradation<i>nag</i> operon |
| spellingShingle | Olesya I. Sazonova Anastasia A. Ivanova Yanina A. Delegan Rostislav A. Streletskii Diana D. Vershinina Sergei L. Sokolov Anna A. Vetrova Characterization and Genomic Analysis of the Naphthalene-Degrading <i>Delftia tsuruhatensis</i> ULwDis3 Isolated from Seawater Microorganisms <i>Delftia tsuruhatensis</i> genome naphthalene biodegradation <i>nag</i> operon |
| title | Characterization and Genomic Analysis of the Naphthalene-Degrading <i>Delftia tsuruhatensis</i> ULwDis3 Isolated from Seawater |
| title_full | Characterization and Genomic Analysis of the Naphthalene-Degrading <i>Delftia tsuruhatensis</i> ULwDis3 Isolated from Seawater |
| title_fullStr | Characterization and Genomic Analysis of the Naphthalene-Degrading <i>Delftia tsuruhatensis</i> ULwDis3 Isolated from Seawater |
| title_full_unstemmed | Characterization and Genomic Analysis of the Naphthalene-Degrading <i>Delftia tsuruhatensis</i> ULwDis3 Isolated from Seawater |
| title_short | Characterization and Genomic Analysis of the Naphthalene-Degrading <i>Delftia tsuruhatensis</i> ULwDis3 Isolated from Seawater |
| title_sort | characterization and genomic analysis of the naphthalene degrading i delftia tsuruhatensis i ulwdis3 isolated from seawater |
| topic | <i>Delftia tsuruhatensis</i> genome naphthalene biodegradation <i>nag</i> operon |
| url | https://www.mdpi.com/2076-2607/11/4/1092 |
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