Ablation of Red Stable Transfected Claudin Expressing Canine Prostate Adenocarcinoma and Transitional Cell Carcinoma Cell Lines by C-CPE Gold-Nanoparticle-Mediated Laser Intervention
Claudin (CLDN) proteins are commonly expressed in cancers and targeted in novel therapeutic approaches. The C-terminal of <i>Clostridium perfringens</i> enterotoxin (C-CPE) efficiently binds several claudins. In this study, recombinant C-CPE conjugated to gold nanoparticles (AuNPs) has b...
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| Language: | English |
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MDPI AG
2021-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/22/12289 |
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| author | Suhayla Alnajjar Ingo Nolte Annegret Becker Jan Torben Schille Nares Trakooljul Marcus Frank Anaclet Ngezahayo Hugo Murua Escobar |
| author_facet | Suhayla Alnajjar Ingo Nolte Annegret Becker Jan Torben Schille Nares Trakooljul Marcus Frank Anaclet Ngezahayo Hugo Murua Escobar |
| author_sort | Suhayla Alnajjar |
| collection | DOAJ |
| description | Claudin (CLDN) proteins are commonly expressed in cancers and targeted in novel therapeutic approaches. The C-terminal of <i>Clostridium perfringens</i> enterotoxin (C-CPE) efficiently binds several claudins. In this study, recombinant C-CPE conjugated to gold nanoparticles (AuNPs) has been used for prostate adenocarcinoma (PAC) and transitional cell carcinoma (TCC) cell killing in vitro using gold-nanoparticle-mediated laser perforation (GNOME-LP). A PAC and TCC cell lines, as well as red fluorescence variants, allowing deep tissue imaging, were used. CLDN-3, -4, and -7 expression was confirmed by qPCR and immunofluorescences. The binding of C-CPE-AuNPs complexes on the cell surface was examined by scanning electron microscopy (SEM). Further, transcriptome analysis was carried out to evaluate the effect of C-CPE binder on the biological response of treated cells. Directed C-CPE-AuNP binding verified the capability to target CLDN receptors. Transcriptome analysis showed that C-CPE binding may activate immune and inflammatory responses but does not directly affect cell survival. Cancer cells ablation was demonstrated using a combination of GNOME-LP and C-CPE-AuNPs treatment reducing tumor cell viability to less than 10% depending on cell line. The fluorescent cell lines and the verified proof of concept in vitro provide the basis for perspective xenograft studies in an animal model. |
| first_indexed | 2024-03-10T05:25:17Z |
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| id | doaj.art-0e116d1d8c1843d6b0d3ee07971c7aac |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T05:25:17Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-0e116d1d8c1843d6b0d3ee07971c7aac2023-11-22T23:39:59ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-11-0122221228910.3390/ijms222212289Ablation of Red Stable Transfected Claudin Expressing Canine Prostate Adenocarcinoma and Transitional Cell Carcinoma Cell Lines by C-CPE Gold-Nanoparticle-Mediated Laser InterventionSuhayla Alnajjar0Ingo Nolte1Annegret Becker2Jan Torben Schille3Nares Trakooljul4Marcus Frank5Anaclet Ngezahayo6Hugo Murua Escobar7Small Animal Clinic, University of Veterinary Medicine Hannover, 30559 Hannover, GermanySmall Animal Clinic, University of Veterinary Medicine Hannover, 30559 Hannover, GermanyInstitute of Cell Biology and Biophysics, Leibniz University Hannover, 30419 Hannover, GermanySmall Animal Clinic, University of Veterinary Medicine Hannover, 30559 Hannover, GermanyResearch Institute for Farm Animal Biology (FBN), Institute of Genome Biology, 18196 Dummerstorf, GermanyDivision of Medicine, Medical Biology and Electron Microscopy Centre, University of Rostock, 18057 Rostock, GermanyInstitute of Cell Biology and Biophysics, Leibniz University Hannover, 30419 Hannover, GermanyDivision of Medicine, Haematology, Oncology and Palliative Medicine, University of Rostock, 18057 Rostock, GermanyClaudin (CLDN) proteins are commonly expressed in cancers and targeted in novel therapeutic approaches. The C-terminal of <i>Clostridium perfringens</i> enterotoxin (C-CPE) efficiently binds several claudins. In this study, recombinant C-CPE conjugated to gold nanoparticles (AuNPs) has been used for prostate adenocarcinoma (PAC) and transitional cell carcinoma (TCC) cell killing in vitro using gold-nanoparticle-mediated laser perforation (GNOME-LP). A PAC and TCC cell lines, as well as red fluorescence variants, allowing deep tissue imaging, were used. CLDN-3, -4, and -7 expression was confirmed by qPCR and immunofluorescences. The binding of C-CPE-AuNPs complexes on the cell surface was examined by scanning electron microscopy (SEM). Further, transcriptome analysis was carried out to evaluate the effect of C-CPE binder on the biological response of treated cells. Directed C-CPE-AuNP binding verified the capability to target CLDN receptors. Transcriptome analysis showed that C-CPE binding may activate immune and inflammatory responses but does not directly affect cell survival. Cancer cells ablation was demonstrated using a combination of GNOME-LP and C-CPE-AuNPs treatment reducing tumor cell viability to less than 10% depending on cell line. The fluorescent cell lines and the verified proof of concept in vitro provide the basis for perspective xenograft studies in an animal model.https://www.mdpi.com/1422-0067/22/22/12289cell linesprostate cancerC-CPEgold nanoparticleGNOME-LP |
| spellingShingle | Suhayla Alnajjar Ingo Nolte Annegret Becker Jan Torben Schille Nares Trakooljul Marcus Frank Anaclet Ngezahayo Hugo Murua Escobar Ablation of Red Stable Transfected Claudin Expressing Canine Prostate Adenocarcinoma and Transitional Cell Carcinoma Cell Lines by C-CPE Gold-Nanoparticle-Mediated Laser Intervention International Journal of Molecular Sciences cell lines prostate cancer C-CPE gold nanoparticle GNOME-LP |
| title | Ablation of Red Stable Transfected Claudin Expressing Canine Prostate Adenocarcinoma and Transitional Cell Carcinoma Cell Lines by C-CPE Gold-Nanoparticle-Mediated Laser Intervention |
| title_full | Ablation of Red Stable Transfected Claudin Expressing Canine Prostate Adenocarcinoma and Transitional Cell Carcinoma Cell Lines by C-CPE Gold-Nanoparticle-Mediated Laser Intervention |
| title_fullStr | Ablation of Red Stable Transfected Claudin Expressing Canine Prostate Adenocarcinoma and Transitional Cell Carcinoma Cell Lines by C-CPE Gold-Nanoparticle-Mediated Laser Intervention |
| title_full_unstemmed | Ablation of Red Stable Transfected Claudin Expressing Canine Prostate Adenocarcinoma and Transitional Cell Carcinoma Cell Lines by C-CPE Gold-Nanoparticle-Mediated Laser Intervention |
| title_short | Ablation of Red Stable Transfected Claudin Expressing Canine Prostate Adenocarcinoma and Transitional Cell Carcinoma Cell Lines by C-CPE Gold-Nanoparticle-Mediated Laser Intervention |
| title_sort | ablation of red stable transfected claudin expressing canine prostate adenocarcinoma and transitional cell carcinoma cell lines by c cpe gold nanoparticle mediated laser intervention |
| topic | cell lines prostate cancer C-CPE gold nanoparticle GNOME-LP |
| url | https://www.mdpi.com/1422-0067/22/22/12289 |
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