From common biomass materials to high-performance tissue engineering scaffold: Biomimetic preparation, properties characterization, in vitro and in vivo evaluations
Converting common biomass materials to high-performance biomedical products could not only reduce the environmental pressure associated with the large-scale use of synthetic materials, but also increase the economic value. Chitosan as a very promising candidate has drawn considerable attention owing...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2024-05-01
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| Series: | Journal of Bioresources and Bioproducts |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2369969824000215 |
| _version_ | 1827284821196406784 |
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| author | Zongpu Xu Fang He Jing Yu Zhangze Yang Yu Zhu Rong Liao Ruyin Lyu Mei Yang Liangjun Zhu Mingying Yang |
| author_facet | Zongpu Xu Fang He Jing Yu Zhangze Yang Yu Zhu Rong Liao Ruyin Lyu Mei Yang Liangjun Zhu Mingying Yang |
| author_sort | Zongpu Xu |
| collection | DOAJ |
| description | Converting common biomass materials to high-performance biomedical products could not only reduce the environmental pressure associated with the large-scale use of synthetic materials, but also increase the economic value. Chitosan as a very promising candidate has drawn considerable attention owing to its abundant sources and remarkable bioactivities. However, pure chitosan materials usually exhibit insufficient mechanical properties and excessive swelling ratio, which seriously affected their in vivo stability and integrity when applied as tissue engineering scaffolds. Thus, simultaneously improving the mechanical strength and biological compatibility of pure chitosan (CS) scaffolds becomes very important. Here, inspired by the fiber-reinforced construction of natural extracellular matrix and the porous structure of cancellous bone, we built silk microfibers/chitosan composite scaffolds via ice-templating technique. This biomimetic strategy achieved 500% of mechanical improvement to pure chitosan, and meanwhile still maintaining high porosity (> 87%). In addition, the increased roughness of chitosan pore walls by embedded silk microfibers significantly promoted cell adhesion and proliferation. More importantly, after subcutaneous implantation in mice for four weeks, the composite scaffold showed greater structural integrity, as well as better collagenation, angiogenesis, and osteogenesis abilities, suggesting its great potential in biomedicine. |
| first_indexed | 2024-04-24T10:03:46Z |
| format | Article |
| id | doaj.art-0e1341f7e44e41769ca561b4ef751b60 |
| institution | Directory Open Access Journal |
| issn | 2369-9698 |
| language | English |
| last_indexed | 2024-04-24T10:03:46Z |
| publishDate | 2024-05-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Bioresources and Bioproducts |
| spelling | doaj.art-0e1341f7e44e41769ca561b4ef751b602024-04-13T04:21:26ZengKeAi Communications Co., Ltd.Journal of Bioresources and Bioproducts2369-96982024-05-0192185196From common biomass materials to high-performance tissue engineering scaffold: Biomimetic preparation, properties characterization, in vitro and in vivo evaluationsZongpu Xu0Fang He1Jing Yu2Zhangze Yang3Yu Zhu4Rong Liao5Ruyin Lyu6Mei Yang7Liangjun Zhu8Mingying Yang9Institute of Applied Bioresources, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China; Key Laboratory of Silkworm and Bee Resource Utilization and Innovation of Zhejiang Province, Zhejiang University, Hangzhou 310058, China; Corresponding author.Institute of Applied Bioresources, College of Animal Sciences, Zhejiang University, Hangzhou 310058, ChinaInstitute of Applied Bioresources, College of Animal Sciences, Zhejiang University, Hangzhou 310058, ChinaInstitute of Applied Bioresources, College of Animal Sciences, Zhejiang University, Hangzhou 310058, ChinaDepartment of Neurosurgery, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, ChinaInstitute of Applied Bioresources, College of Animal Sciences, Zhejiang University, Hangzhou 310058, ChinaInstitute of Applied Bioresources, College of Animal Sciences, Zhejiang University, Hangzhou 310058, ChinaInstitute of Applied Bioresources, College of Animal Sciences, Zhejiang University, Hangzhou 310058, ChinaInstitute of Applied Bioresources, College of Animal Sciences, Zhejiang University, Hangzhou 310058, ChinaInstitute of Applied Bioresources, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China; Key Laboratory of Silkworm and Bee Resource Utilization and Innovation of Zhejiang Province, Zhejiang University, Hangzhou 310058, ChinaConverting common biomass materials to high-performance biomedical products could not only reduce the environmental pressure associated with the large-scale use of synthetic materials, but also increase the economic value. Chitosan as a very promising candidate has drawn considerable attention owing to its abundant sources and remarkable bioactivities. However, pure chitosan materials usually exhibit insufficient mechanical properties and excessive swelling ratio, which seriously affected their in vivo stability and integrity when applied as tissue engineering scaffolds. Thus, simultaneously improving the mechanical strength and biological compatibility of pure chitosan (CS) scaffolds becomes very important. Here, inspired by the fiber-reinforced construction of natural extracellular matrix and the porous structure of cancellous bone, we built silk microfibers/chitosan composite scaffolds via ice-templating technique. This biomimetic strategy achieved 500% of mechanical improvement to pure chitosan, and meanwhile still maintaining high porosity (> 87%). In addition, the increased roughness of chitosan pore walls by embedded silk microfibers significantly promoted cell adhesion and proliferation. More importantly, after subcutaneous implantation in mice for four weeks, the composite scaffold showed greater structural integrity, as well as better collagenation, angiogenesis, and osteogenesis abilities, suggesting its great potential in biomedicine.http://www.sciencedirect.com/science/article/pii/S2369969824000215ChitosanBiomimetic strategyFiber-reinforced compositeMechanical propertyBiocompatibility |
| spellingShingle | Zongpu Xu Fang He Jing Yu Zhangze Yang Yu Zhu Rong Liao Ruyin Lyu Mei Yang Liangjun Zhu Mingying Yang From common biomass materials to high-performance tissue engineering scaffold: Biomimetic preparation, properties characterization, in vitro and in vivo evaluations Journal of Bioresources and Bioproducts Chitosan Biomimetic strategy Fiber-reinforced composite Mechanical property Biocompatibility |
| title | From common biomass materials to high-performance tissue engineering scaffold: Biomimetic preparation, properties characterization, in vitro and in vivo evaluations |
| title_full | From common biomass materials to high-performance tissue engineering scaffold: Biomimetic preparation, properties characterization, in vitro and in vivo evaluations |
| title_fullStr | From common biomass materials to high-performance tissue engineering scaffold: Biomimetic preparation, properties characterization, in vitro and in vivo evaluations |
| title_full_unstemmed | From common biomass materials to high-performance tissue engineering scaffold: Biomimetic preparation, properties characterization, in vitro and in vivo evaluations |
| title_short | From common biomass materials to high-performance tissue engineering scaffold: Biomimetic preparation, properties characterization, in vitro and in vivo evaluations |
| title_sort | from common biomass materials to high performance tissue engineering scaffold biomimetic preparation properties characterization in vitro and in vivo evaluations |
| topic | Chitosan Biomimetic strategy Fiber-reinforced composite Mechanical property Biocompatibility |
| url | http://www.sciencedirect.com/science/article/pii/S2369969824000215 |
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