Comparative Analysis of Genetic Similarity between Perennial Ryegrass Genotypes Investigated With AFLPs, ISSRs, RAPDs and SSRs
Perennial ryegrass (Lolium perenne L.) is the most important grass species used in temperate grassland agriculture. Our objective was to obtain an overview of the genetic relationships between 20 individual genotypes of perennial ryegrass of diverse origins, using amplified fragment length polymorph...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2006-09-01
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| Series: | Czech Journal of Genetics and Plant Breeding |
| Subjects: | |
| Online Access: | https://cjgpb.agriculturejournals.cz/artkey/cjg-200603-0002_comparative-analysis-of-genetic-similarity-between-perennial-ryegrass-genotypes-investigated-with-aflps-issrs.php |
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| author | Ulrich Kurt Posselt Philippe Barre Gintaras Brazauskas Lesley Beryl Turner |
| author_facet | Ulrich Kurt Posselt Philippe Barre Gintaras Brazauskas Lesley Beryl Turner |
| author_sort | Ulrich Kurt Posselt |
| collection | DOAJ |
| description | Perennial ryegrass (Lolium perenne L.) is the most important grass species used in temperate grassland agriculture. Our objective was to obtain an overview of the genetic relationships between 20 individual genotypes of perennial ryegrass of diverse origins, using amplified fragment length polymorphism (AFLP), inter-simple sequence repeat (ISSR), random amplified polymorphic DNA (RAPD) and two sets of simple sequence repeat (SSR) markers. All 20 individuals were uniquely fingerprinted by all four marker systems and comparisons were made on the basis of 85 markers each. Mean genetic similarities were estimated at 0.31, 0.43, 0.23 and 0.15 for AFLPs, ISSRs, RAPDs and SSRs, respectively. Cophenetic values resulted in good (AFLP and SSR-B = 0.88) to moderately good fits (ISSR = 0.76, RAPD = 0.70, and SSR-A = 0.79). Comparing the four marker systems to each other, AFLP and SSR-A were correlated best (r = 0.57). All other comparisons revealed rather low correlation coefficients in the Mantel Z test. With twice as many markers cophenetic values increased to a very good fit for AFLPs (0.90) and SSRs (0.92). |
| first_indexed | 2024-04-10T08:30:09Z |
| format | Article |
| id | doaj.art-0e14f3c1ed0049b7965e0774c8160064 |
| institution | Directory Open Access Journal |
| issn | 1212-1975 1805-9325 |
| language | English |
| last_indexed | 2024-04-10T08:30:09Z |
| publishDate | 2006-09-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Genetics and Plant Breeding |
| spelling | doaj.art-0e14f3c1ed0049b7965e0774c81600642023-02-23T03:29:14ZengCzech Academy of Agricultural SciencesCzech Journal of Genetics and Plant Breeding1212-19751805-93252006-09-01423879410.17221/3647-CJGPBcjg-200603-0002Comparative Analysis of Genetic Similarity between Perennial Ryegrass Genotypes Investigated With AFLPs, ISSRs, RAPDs and SSRsUlrich Kurt Posselt0Philippe Barre1Gintaras Brazauskas2Lesley Beryl Turner3State Plant Breeding Institute, University of Hohenheim, Stuttgart, GermanyNational Institute of Agronomic Research (INRA), Forage Genetics and Breeding Unit (UGAPF), Lusignan, FranceLithuanian Institute of Agriculture, Akademija, Kėdainiai, LithuaniaInstitute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth, Ceredigion, United KingdomPerennial ryegrass (Lolium perenne L.) is the most important grass species used in temperate grassland agriculture. Our objective was to obtain an overview of the genetic relationships between 20 individual genotypes of perennial ryegrass of diverse origins, using amplified fragment length polymorphism (AFLP), inter-simple sequence repeat (ISSR), random amplified polymorphic DNA (RAPD) and two sets of simple sequence repeat (SSR) markers. All 20 individuals were uniquely fingerprinted by all four marker systems and comparisons were made on the basis of 85 markers each. Mean genetic similarities were estimated at 0.31, 0.43, 0.23 and 0.15 for AFLPs, ISSRs, RAPDs and SSRs, respectively. Cophenetic values resulted in good (AFLP and SSR-B = 0.88) to moderately good fits (ISSR = 0.76, RAPD = 0.70, and SSR-A = 0.79). Comparing the four marker systems to each other, AFLP and SSR-A were correlated best (r = 0.57). All other comparisons revealed rather low correlation coefficients in the Mantel Z test. With twice as many markers cophenetic values increased to a very good fit for AFLPs (0.90) and SSRs (0.92).https://cjgpb.agriculturejournals.cz/artkey/cjg-200603-0002_comparative-analysis-of-genetic-similarity-between-perennial-ryegrass-genotypes-investigated-with-aflps-issrs.phpmarker comparisonaflpissrrapdssrlolium perenne l |
| spellingShingle | Ulrich Kurt Posselt Philippe Barre Gintaras Brazauskas Lesley Beryl Turner Comparative Analysis of Genetic Similarity between Perennial Ryegrass Genotypes Investigated With AFLPs, ISSRs, RAPDs and SSRs Czech Journal of Genetics and Plant Breeding marker comparison aflp issr rapd ssr lolium perenne l |
| title | Comparative Analysis of Genetic Similarity between Perennial Ryegrass Genotypes Investigated With AFLPs, ISSRs, RAPDs and SSRs |
| title_full | Comparative Analysis of Genetic Similarity between Perennial Ryegrass Genotypes Investigated With AFLPs, ISSRs, RAPDs and SSRs |
| title_fullStr | Comparative Analysis of Genetic Similarity between Perennial Ryegrass Genotypes Investigated With AFLPs, ISSRs, RAPDs and SSRs |
| title_full_unstemmed | Comparative Analysis of Genetic Similarity between Perennial Ryegrass Genotypes Investigated With AFLPs, ISSRs, RAPDs and SSRs |
| title_short | Comparative Analysis of Genetic Similarity between Perennial Ryegrass Genotypes Investigated With AFLPs, ISSRs, RAPDs and SSRs |
| title_sort | comparative analysis of genetic similarity between perennial ryegrass genotypes investigated with aflps issrs rapds and ssrs |
| topic | marker comparison aflp issr rapd ssr lolium perenne l |
| url | https://cjgpb.agriculturejournals.cz/artkey/cjg-200603-0002_comparative-analysis-of-genetic-similarity-between-perennial-ryegrass-genotypes-investigated-with-aflps-issrs.php |
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