Diallyl disulfide attenuates acetaminophen-induced renal injury in rats
Abstract This study investigated the protective effects of diallyl disulfide (DADS) against acetaminophen (AAP)-induced acute renal injury in male rats. We also investigated the effects of DADS on kidney injury molecule-1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NCAL), which are novel...
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| Format: | Article |
| Language: | English |
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BMC
2016-12-01
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| Series: | Laboratory Animal Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.5625/lar.2016.32.4.200 |
| _version_ | 1818131549009215488 |
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| author | Jin-Young Shin Ji-Hee Han Je-Won Ko Sung-Hyeuk Park Na-Rae Shin Tae-Yang Jung Hyun-A Kim Sung-Hwan Kim In-Sik Shin Jong-Choon Kim |
| author_facet | Jin-Young Shin Ji-Hee Han Je-Won Ko Sung-Hyeuk Park Na-Rae Shin Tae-Yang Jung Hyun-A Kim Sung-Hwan Kim In-Sik Shin Jong-Choon Kim |
| author_sort | Jin-Young Shin |
| collection | DOAJ |
| description | Abstract This study investigated the protective effects of diallyl disulfide (DADS) against acetaminophen (AAP)-induced acute renal injury in male rats. We also investigated the effects of DADS on kidney injury molecule-1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NCAL), which are novel biomarkers of nephrotoxicity in renal tissues, in response to AAP treatment. The following four experimental groups were evaluated: (1) vehicle control, (2) AAP (1,000 mg/kg), (3) AAP&DADS, and (4) DADS (50 mg/kg/day). AAP treatment caused acute kidney injury evidenced by increased serum blood urea nitrogen (BUN) levels and histopathological alterations. Additionally, Western blot and immunohistochemistry analysis showed increased expression of KIM-1 and NCAL proteins in renal tissues of AAP-treated rats. In contrast, DADS pretreatment significantly attenuated the AAP-induced nephrotoxic effects, including serum BUN level and expression of KIM-1 and NCAL proteins. Histopathological studies confirmed the renoprotective effect of DADS. The results suggest that DADS prevents AAP-induced acute nephrotoxicity, and that KIM-1 and NCAL may be useful biomarkers for the detection and monitoring of acute kidney injury associated with AAP exposure. |
| first_indexed | 2024-12-11T08:22:42Z |
| format | Article |
| id | doaj.art-0e16714d8fa542fdb800af453658ef49 |
| institution | Directory Open Access Journal |
| issn | 2233-7660 |
| language | English |
| last_indexed | 2024-12-11T08:22:42Z |
| publishDate | 2016-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Laboratory Animal Research |
| spelling | doaj.art-0e16714d8fa542fdb800af453658ef492022-12-22T01:14:37ZengBMCLaboratory Animal Research2233-76602016-12-0132420020710.5625/lar.2016.32.4.200Diallyl disulfide attenuates acetaminophen-induced renal injury in ratsJin-Young Shin0Ji-Hee Han1Je-Won Ko2Sung-Hyeuk Park3Na-Rae Shin4Tae-Yang Jung5Hyun-A Kim6Sung-Hwan Kim7In-Sik Shin8Jong-Choon Kim9Ministry of Food and Drug SafetyCollege of Veterinary Medicine, Chonnam National UniversityCollege of Veterinary Medicine, Chonnam National UniversityCollege of Veterinary Medicine, Chonnam National UniversityCollege of Veterinary Medicine, Chonnam National UniversityCollege of Veterinary Medicine, Chonnam National UniversityCollege of Veterinary Medicine, Chonnam National UniversityJeonbuk Department of Inhalation Research, Korea Institute of ToxicologyCollege of Veterinary Medicine, Chonnam National UniversityCollege of Veterinary Medicine, Chonnam National UniversityAbstract This study investigated the protective effects of diallyl disulfide (DADS) against acetaminophen (AAP)-induced acute renal injury in male rats. We also investigated the effects of DADS on kidney injury molecule-1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NCAL), which are novel biomarkers of nephrotoxicity in renal tissues, in response to AAP treatment. The following four experimental groups were evaluated: (1) vehicle control, (2) AAP (1,000 mg/kg), (3) AAP&DADS, and (4) DADS (50 mg/kg/day). AAP treatment caused acute kidney injury evidenced by increased serum blood urea nitrogen (BUN) levels and histopathological alterations. Additionally, Western blot and immunohistochemistry analysis showed increased expression of KIM-1 and NCAL proteins in renal tissues of AAP-treated rats. In contrast, DADS pretreatment significantly attenuated the AAP-induced nephrotoxic effects, including serum BUN level and expression of KIM-1 and NCAL proteins. Histopathological studies confirmed the renoprotective effect of DADS. The results suggest that DADS prevents AAP-induced acute nephrotoxicity, and that KIM-1 and NCAL may be useful biomarkers for the detection and monitoring of acute kidney injury associated with AAP exposure.http://link.springer.com/article/10.5625/lar.2016.32.4.200AcetaminophennephrotoxicityKIM-1NCALdiallyl disulfideprotective effect |
| spellingShingle | Jin-Young Shin Ji-Hee Han Je-Won Ko Sung-Hyeuk Park Na-Rae Shin Tae-Yang Jung Hyun-A Kim Sung-Hwan Kim In-Sik Shin Jong-Choon Kim Diallyl disulfide attenuates acetaminophen-induced renal injury in rats Laboratory Animal Research Acetaminophen nephrotoxicity KIM-1 NCAL diallyl disulfide protective effect |
| title | Diallyl disulfide attenuates acetaminophen-induced renal injury in rats |
| title_full | Diallyl disulfide attenuates acetaminophen-induced renal injury in rats |
| title_fullStr | Diallyl disulfide attenuates acetaminophen-induced renal injury in rats |
| title_full_unstemmed | Diallyl disulfide attenuates acetaminophen-induced renal injury in rats |
| title_short | Diallyl disulfide attenuates acetaminophen-induced renal injury in rats |
| title_sort | diallyl disulfide attenuates acetaminophen induced renal injury in rats |
| topic | Acetaminophen nephrotoxicity KIM-1 NCAL diallyl disulfide protective effect |
| url | http://link.springer.com/article/10.5625/lar.2016.32.4.200 |
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