Production of Bioactive Peptides from Baltic Herring (<i>Clupea harengus membras</i>): Dipeptidyl Peptidase-4 Inhibitory, Antioxidant and Antiproliferative Properties
This study aimed to produce bioactive protein hydrolysates from undervalued fish, namely Baltic herring, and its filleting by-products. Protein hydrolysates were produced with Alcalase and Flavourzyme to achieve effective hydrolysis. The hydrolysates were evaluated for chemical composition, molecula...
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MDPI AG
2022-09-01
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| author | Sari Mäkinen Jaakko Hiidenhovi Xin Huang Amanda dos Santos Lima Luciana Azevedo Jari Setälä Anna-Liisa Välimaa Pirjo Mattila Daniel Granato |
| author_facet | Sari Mäkinen Jaakko Hiidenhovi Xin Huang Amanda dos Santos Lima Luciana Azevedo Jari Setälä Anna-Liisa Välimaa Pirjo Mattila Daniel Granato |
| author_sort | Sari Mäkinen |
| collection | DOAJ |
| description | This study aimed to produce bioactive protein hydrolysates from undervalued fish, namely Baltic herring, and its filleting by-products. Protein hydrolysates were produced with Alcalase and Flavourzyme to achieve effective hydrolysis. The hydrolysates were evaluated for chemical composition, molecular weight distribution, antioxidant capacity, dipeptidyl-peptidase 4 (DPP4) inhibitory activity, effects on cell proliferation and surface hydrophobicity. The protein content of the hydrolysates was high, from 86% to 91% (dm), while the fat content was low, from 0.3% to 0.4% (dm). The hydrolysates showed high DPP4 inhibition activities with IC<sub>50</sub> values from 5.38 mg/mL to 7.92 mg/mL. The scavenging activity of the hydrolysates towards DPPH was low, but an intermediate Folin–Ciocalteu reducing capacity and Cu<sup>2+</sup> chelating ability was observed. The solid phase extraction with Sep-Pak C18 cartridges increased the DPP4 inhibition activity and antioxidant capacity, indicating peptides’ crucial role in the bioactivities. The cytotoxicity of the hydrolysates was evaluated on the HCT8, IMR90, and A549 cell lines. The hydrolysates inhibited cell growth in the cancer and normal cells, although they did not reduce cell viability and were not lethal. Overall, our results indicate that protein hydrolysates from Baltic herring have potential as health-promoting foods and nutraceuticals, especially for enhancing healthy blood glucose regulation. |
| first_indexed | 2024-03-09T23:02:39Z |
| format | Article |
| id | doaj.art-0e1692c33721401ca4248bc90a169287 |
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| language | English |
| last_indexed | 2024-03-09T23:02:39Z |
| publishDate | 2022-09-01 |
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| series | Molecules |
| spelling | doaj.art-0e1692c33721401ca4248bc90a1692872023-11-23T17:59:23ZengMDPI AGMolecules1420-30492022-09-012718581610.3390/molecules27185816Production of Bioactive Peptides from Baltic Herring (<i>Clupea harengus membras</i>): Dipeptidyl Peptidase-4 Inhibitory, Antioxidant and Antiproliferative PropertiesSari Mäkinen0Jaakko Hiidenhovi1Xin Huang2Amanda dos Santos Lima3Luciana Azevedo4Jari Setälä5Anna-Liisa Välimaa6Pirjo Mattila7Daniel Granato8Production Systems Unit—Natural Resources Institute Finland (Luke), FI-31600 Jokioinen, FinlandProduction Systems Unit—Natural Resources Institute Finland (Luke), FI-31600 Jokioinen, FinlandProduction Systems Unit—Natural Resources Institute Finland (Luke), FI-31600 Jokioinen, FinlandFaculty of Nutrition, Federal University of Alfenas, Rua Gabriel Monteiro da Silva, 714, Alfenas 37130-000, BrazilFaculty of Nutrition, Federal University of Alfenas, Rua Gabriel Monteiro da Silva, 714, Alfenas 37130-000, BrazilProduction Systems Unit—Natural Resources Institute Finland (Luke), FI-20520 Turku, FinlandProduction Systems Unit—Natural Resources Institute Finland (Luke), University of Oulu, FI-90014 Oulu, FinlandProduction Systems Unit—Natural Resources Institute Finland (Luke), FI-20520 Turku, FinlandBioactivity and Applications Lab, Department of Biological Sciences, Faculty of Science and Engineering, University of Limerick, V94 T9PX Limerick, IrelandThis study aimed to produce bioactive protein hydrolysates from undervalued fish, namely Baltic herring, and its filleting by-products. Protein hydrolysates were produced with Alcalase and Flavourzyme to achieve effective hydrolysis. The hydrolysates were evaluated for chemical composition, molecular weight distribution, antioxidant capacity, dipeptidyl-peptidase 4 (DPP4) inhibitory activity, effects on cell proliferation and surface hydrophobicity. The protein content of the hydrolysates was high, from 86% to 91% (dm), while the fat content was low, from 0.3% to 0.4% (dm). The hydrolysates showed high DPP4 inhibition activities with IC<sub>50</sub> values from 5.38 mg/mL to 7.92 mg/mL. The scavenging activity of the hydrolysates towards DPPH was low, but an intermediate Folin–Ciocalteu reducing capacity and Cu<sup>2+</sup> chelating ability was observed. The solid phase extraction with Sep-Pak C18 cartridges increased the DPP4 inhibition activity and antioxidant capacity, indicating peptides’ crucial role in the bioactivities. The cytotoxicity of the hydrolysates was evaluated on the HCT8, IMR90, and A549 cell lines. The hydrolysates inhibited cell growth in the cancer and normal cells, although they did not reduce cell viability and were not lethal. Overall, our results indicate that protein hydrolysates from Baltic herring have potential as health-promoting foods and nutraceuticals, especially for enhancing healthy blood glucose regulation.https://www.mdpi.com/1420-3049/27/18/5816bioactive peptidesantioxidantsantidiabetic effectscell-based experimentsreactive oxygen species |
| spellingShingle | Sari Mäkinen Jaakko Hiidenhovi Xin Huang Amanda dos Santos Lima Luciana Azevedo Jari Setälä Anna-Liisa Välimaa Pirjo Mattila Daniel Granato Production of Bioactive Peptides from Baltic Herring (<i>Clupea harengus membras</i>): Dipeptidyl Peptidase-4 Inhibitory, Antioxidant and Antiproliferative Properties Molecules bioactive peptides antioxidants antidiabetic effects cell-based experiments reactive oxygen species |
| title | Production of Bioactive Peptides from Baltic Herring (<i>Clupea harengus membras</i>): Dipeptidyl Peptidase-4 Inhibitory, Antioxidant and Antiproliferative Properties |
| title_full | Production of Bioactive Peptides from Baltic Herring (<i>Clupea harengus membras</i>): Dipeptidyl Peptidase-4 Inhibitory, Antioxidant and Antiproliferative Properties |
| title_fullStr | Production of Bioactive Peptides from Baltic Herring (<i>Clupea harengus membras</i>): Dipeptidyl Peptidase-4 Inhibitory, Antioxidant and Antiproliferative Properties |
| title_full_unstemmed | Production of Bioactive Peptides from Baltic Herring (<i>Clupea harengus membras</i>): Dipeptidyl Peptidase-4 Inhibitory, Antioxidant and Antiproliferative Properties |
| title_short | Production of Bioactive Peptides from Baltic Herring (<i>Clupea harengus membras</i>): Dipeptidyl Peptidase-4 Inhibitory, Antioxidant and Antiproliferative Properties |
| title_sort | production of bioactive peptides from baltic herring i clupea harengus membras i dipeptidyl peptidase 4 inhibitory antioxidant and antiproliferative properties |
| topic | bioactive peptides antioxidants antidiabetic effects cell-based experiments reactive oxygen species |
| url | https://www.mdpi.com/1420-3049/27/18/5816 |
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