Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections
An increasing amount of research has been conducted on immunoglobulin Y (IgY) because the use of IgY offers several advantages with respect to diagnostic testing, including its easy accessibility, low cost and translatability to large-scale production, in addition to the fact that it can be ethicall...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Fundação Oswaldo Cruz (FIOCRUZ)
2015-06-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000400577&lng=en&tlng=en |
| _version_ | 1797718334294196224 |
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| author | Gentil Arthur Bentes Natália Maria Lanzarini Lyana Rodrigues Pinto Lima Pedro Paulo de Abreu Manso Alexandre dos Santos da Silva Sergio da Silva e Mouta Junior Juliana Rodrigues Guimarães Marcia Terezinha Baroni de Moraes Marcelo Pelajo-Machado Marcelo Alves Pinto |
| author_facet | Gentil Arthur Bentes Natália Maria Lanzarini Lyana Rodrigues Pinto Lima Pedro Paulo de Abreu Manso Alexandre dos Santos da Silva Sergio da Silva e Mouta Junior Juliana Rodrigues Guimarães Marcia Terezinha Baroni de Moraes Marcelo Pelajo-Machado Marcelo Alves Pinto |
| author_sort | Gentil Arthur Bentes |
| collection | DOAJ |
| description | An increasing amount of research has been conducted on immunoglobulin Y (IgY) because the use of IgY offers several advantages with respect to diagnostic testing, including its easy accessibility, low cost and translatability to large-scale production, in addition to the fact that it can be ethically produced. In a previous work, immunoglobulin was produced and purified from egg yolks (IgY) reactive to hepatitis A virus (HAV) antigens. In the present work, this anti-HAV-specific IgY was used in an indirect immunofluorescence assay to detect viral antigens in liver biopsies that were obtained from experimentally infected cynomolgus monkeys. Fields that were positive for HAV antigen were detected in liver sections using confocal microscopy. In conclusion, egg yolks from immunised hens may be a reliable source for antibody production, which can be employed for immunological studies. |
| first_indexed | 2024-03-12T08:49:30Z |
| format | Article |
| id | doaj.art-0e1759e3c8de49c4893a791285495bb1 |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:49:30Z |
| publishDate | 2015-06-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-0e1759e3c8de49c4893a791285495bb12023-09-02T16:21:37ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602015-06-01110457757910.1590/0074-02760140457S0074-02762015000400577Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sectionsGentil Arthur BentesNatália Maria LanzariniLyana Rodrigues Pinto LimaPedro Paulo de Abreu MansoAlexandre dos Santos da SilvaSergio da Silva e Mouta JuniorJuliana Rodrigues GuimarãesMarcia Terezinha Baroni de MoraesMarcelo Pelajo-MachadoMarcelo Alves PintoAn increasing amount of research has been conducted on immunoglobulin Y (IgY) because the use of IgY offers several advantages with respect to diagnostic testing, including its easy accessibility, low cost and translatability to large-scale production, in addition to the fact that it can be ethically produced. In a previous work, immunoglobulin was produced and purified from egg yolks (IgY) reactive to hepatitis A virus (HAV) antigens. In the present work, this anti-HAV-specific IgY was used in an indirect immunofluorescence assay to detect viral antigens in liver biopsies that were obtained from experimentally infected cynomolgus monkeys. Fields that were positive for HAV antigen were detected in liver sections using confocal microscopy. In conclusion, egg yolks from immunised hens may be a reliable source for antibody production, which can be employed for immunological studies.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000400577&lng=en&tlng=enimmunoglobulin Yhepatitis A virusindirect immunofluorescence |
| spellingShingle | Gentil Arthur Bentes Natália Maria Lanzarini Lyana Rodrigues Pinto Lima Pedro Paulo de Abreu Manso Alexandre dos Santos da Silva Sergio da Silva e Mouta Junior Juliana Rodrigues Guimarães Marcia Terezinha Baroni de Moraes Marcelo Pelajo-Machado Marcelo Alves Pinto Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections Memorias do Instituto Oswaldo Cruz immunoglobulin Y hepatitis A virus indirect immunofluorescence |
| title | Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections |
| title_full | Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections |
| title_fullStr | Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections |
| title_full_unstemmed | Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections |
| title_short | Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections |
| title_sort | using immunoglobulin y as an alternative antibody for the detection of hepatitis a virus in frozen liver sections |
| topic | immunoglobulin Y hepatitis A virus indirect immunofluorescence |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000400577&lng=en&tlng=en |
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