Phosphoenolpyruvate phosphotransferase system components positively regulate Klebsiella biofilm formation
Background/Purpose: Klebsiella pneumoniae is one of the leading causes of device-related infections (DRIs), which are associated with attachment of bacteria to these devices to form a biofilm. The latter is composed of not only bacteria but also extracellular polymeric substances (EPSes) consisting...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2018-04-01
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| Series: | Journal of Microbiology, Immunology and Infection |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1684118217301159 |
| _version_ | 1819032504362860544 |
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| author | Yu-Tze Horng Chi-Jen Wang Wen-Ting Chung Huei-Jen Chao Yih-Yuan Chen Po-Chi Soo |
| author_facet | Yu-Tze Horng Chi-Jen Wang Wen-Ting Chung Huei-Jen Chao Yih-Yuan Chen Po-Chi Soo |
| author_sort | Yu-Tze Horng |
| collection | DOAJ |
| description | Background/Purpose: Klebsiella pneumoniae is one of the leading causes of device-related infections (DRIs), which are associated with attachment of bacteria to these devices to form a biofilm. The latter is composed of not only bacteria but also extracellular polymeric substances (EPSes) consisting of extracellular DNAs, polysaccharides, and other macromolecules. The phosphoenolpyruvate (PEP):carbohydrate phosphotransferase system (PTS) regulates diverse processes of bacterial physiology. In the genome of K. pneumoniae MGH 78578, we found an uncharacterized enzyme II complex homolog of PTS: KPN00353 (EIIA homolog), KPN00352 (EIIB homolog), and KPN00351 (EIIC homolog). The aim of this study was to characterize the potential physiological role of KPN00353, KPN00352, and KPN00351 in biofilm formation by K. pneumoniae. Methods/Results: We constructed the PTS mutants and recombinant strains carrying the gene(s) of PTS. The recombinant K. pneumoniae strain overexpressing KPN00353–KPN00352–KPN00351 produced more extracellular matrix than did the vector control according to transmission and scanning electron microscopy. Judging by quantification of biofilm formation, of extracellular DNA (eDNA), and of capsular polysaccharide, the recombinant strain overexpressing KPN00353-KPN00352-KPN00351 produced more biofilm and capsular polysaccharide after overnight culture and more eDNA in the log phase as compared to the vector control. Conclusion: The genes, KPN00353–KPN00352–KPN00351, encode a putative enzyme II complex in PTS and positively regulate biofilm formation by enhancing production of eDNA and capsular polysaccharide in K. pneumoniae. Five proteins related to chaperones, to the citric acid cycle, and to quorum sensing are upregulated by the KPN00353–KPN00352–KPN00351 system. Keywords: Klebsiella, PTS, Biofilm, eDNA, Polysaccharide |
| first_indexed | 2024-12-21T07:03:00Z |
| format | Article |
| id | doaj.art-0e1802c03acd4b138b541838482eb6a2 |
| institution | Directory Open Access Journal |
| issn | 1684-1182 |
| language | English |
| last_indexed | 2024-12-21T07:03:00Z |
| publishDate | 2018-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Microbiology, Immunology and Infection |
| spelling | doaj.art-0e1802c03acd4b138b541838482eb6a22022-12-21T19:12:10ZengElsevierJournal of Microbiology, Immunology and Infection1684-11822018-04-01512174183Phosphoenolpyruvate phosphotransferase system components positively regulate Klebsiella biofilm formationYu-Tze Horng0Chi-Jen Wang1Wen-Ting Chung2Huei-Jen Chao3Yih-Yuan Chen4Po-Chi Soo5Department of Laboratory Medicine and Biotechnology, Tzu Chi University, College of Medicine, 701 Section 3, Zhongyang Road, Hualien 97004, Taiwan, ROCDepartment of Laboratory Medicine and Biotechnology, Tzu Chi University, College of Medicine, 701 Section 3, Zhongyang Road, Hualien 97004, Taiwan, ROCDepartment of Laboratory Medicine and Biotechnology, Tzu Chi University, College of Medicine, 701 Section 3, Zhongyang Road, Hualien 97004, Taiwan, ROCDepartment of Laboratory Medicine, Buddhist Tzu Chi General Hospital, Hualien, Taiwan, ROCDepartment of Biochemical Science and Technology, National Chiayi University, Chiayi 60004, Taiwan, ROCDepartment of Laboratory Medicine and Biotechnology, Tzu Chi University, College of Medicine, 701 Section 3, Zhongyang Road, Hualien 97004, Taiwan, ROC; Corresponding author. Department of Laboratory Medicine and Biotechnology, College of Medicine, Tzu Chi University, No. 701, Section 3, Zhongyang Rd., Hualien 97004, Taiwan, ROC. Fax: +886 3 8571917.Background/Purpose: Klebsiella pneumoniae is one of the leading causes of device-related infections (DRIs), which are associated with attachment of bacteria to these devices to form a biofilm. The latter is composed of not only bacteria but also extracellular polymeric substances (EPSes) consisting of extracellular DNAs, polysaccharides, and other macromolecules. The phosphoenolpyruvate (PEP):carbohydrate phosphotransferase system (PTS) regulates diverse processes of bacterial physiology. In the genome of K. pneumoniae MGH 78578, we found an uncharacterized enzyme II complex homolog of PTS: KPN00353 (EIIA homolog), KPN00352 (EIIB homolog), and KPN00351 (EIIC homolog). The aim of this study was to characterize the potential physiological role of KPN00353, KPN00352, and KPN00351 in biofilm formation by K. pneumoniae. Methods/Results: We constructed the PTS mutants and recombinant strains carrying the gene(s) of PTS. The recombinant K. pneumoniae strain overexpressing KPN00353–KPN00352–KPN00351 produced more extracellular matrix than did the vector control according to transmission and scanning electron microscopy. Judging by quantification of biofilm formation, of extracellular DNA (eDNA), and of capsular polysaccharide, the recombinant strain overexpressing KPN00353-KPN00352-KPN00351 produced more biofilm and capsular polysaccharide after overnight culture and more eDNA in the log phase as compared to the vector control. Conclusion: The genes, KPN00353–KPN00352–KPN00351, encode a putative enzyme II complex in PTS and positively regulate biofilm formation by enhancing production of eDNA and capsular polysaccharide in K. pneumoniae. Five proteins related to chaperones, to the citric acid cycle, and to quorum sensing are upregulated by the KPN00353–KPN00352–KPN00351 system. Keywords: Klebsiella, PTS, Biofilm, eDNA, Polysaccharidehttp://www.sciencedirect.com/science/article/pii/S1684118217301159 |
| spellingShingle | Yu-Tze Horng Chi-Jen Wang Wen-Ting Chung Huei-Jen Chao Yih-Yuan Chen Po-Chi Soo Phosphoenolpyruvate phosphotransferase system components positively regulate Klebsiella biofilm formation Journal of Microbiology, Immunology and Infection |
| title | Phosphoenolpyruvate phosphotransferase system components positively regulate Klebsiella biofilm formation |
| title_full | Phosphoenolpyruvate phosphotransferase system components positively regulate Klebsiella biofilm formation |
| title_fullStr | Phosphoenolpyruvate phosphotransferase system components positively regulate Klebsiella biofilm formation |
| title_full_unstemmed | Phosphoenolpyruvate phosphotransferase system components positively regulate Klebsiella biofilm formation |
| title_short | Phosphoenolpyruvate phosphotransferase system components positively regulate Klebsiella biofilm formation |
| title_sort | phosphoenolpyruvate phosphotransferase system components positively regulate klebsiella biofilm formation |
| url | http://www.sciencedirect.com/science/article/pii/S1684118217301159 |
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