A proteomic approach to analyzing responses of <it>Arabidopsis thaliana </it>root cells to different gravitational conditions using an agravitropic mutant, <it>pin2 </it>and its wild type
<p>Abstract</p> <p>Background</p> <p>Root gravitropsim has been proposed to require the coordinated, redistribution of the plant signaling molecule auxin within the root meristem, but the underlying molecular mechanisms are still unknown. PIN proteins are membrane trans...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2011-11-01
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| Series: | Proteome Science |
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| Online Access: | http://www.proteomesci.com/content/9/1/72 |
| _version_ | 1828424625837572096 |
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| author | Tan Chao Wang Hui Zhang Yue Qi Bin Xu Guoxin Zheng Huiqiong |
| author_facet | Tan Chao Wang Hui Zhang Yue Qi Bin Xu Guoxin Zheng Huiqiong |
| author_sort | Tan Chao |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Root gravitropsim has been proposed to require the coordinated, redistribution of the plant signaling molecule auxin within the root meristem, but the underlying molecular mechanisms are still unknown. PIN proteins are membrane transporters that mediate the efflux of auxin from cells. The PIN2 is important for the basipetal transport of auxin in roots and plays a critical role in the transmission of gravity signals perceived in the root cap to the root elongation zone. The loss of function <it>pin2 </it>mutant exhibits a gravity-insensitive root growth phenotype. By comparing the proteomes of wild type and the <it>pin2 </it>mutant root tips under different gravitational conditions, we hope to identify proteins involved in the gravity-related signal transduction.</p> <p>Results</p> <p>To identify novel proteins involved in the gravity signal transduction pathway we have carried out a comparative proteomic analysis of Arabidopsis <it>pin2 </it>mutant and wild type (WT) roots subjected to different gravitational conditions. These conditions included horizontal (H) and vertical (V) clinorotation, hypergravity (G) and the stationary control (S). Analysis of silver-stained two-dimensional SDS-PAGE gels revealed 28 protein spots that showed significant expression changes in altered gravity (H or G) compared to control roots (V and S). Whereas the majority of these proteins exhibited similar expression patterns in WT and <it>pin2 </it>roots, a significant number displayed different patterns of response between WT and <it>pin2 </it>roots. The latter group included 11 protein spots in the H samples and two protein spots in the G samples that exhibited an altered expression exclusively in WT but not in <it>pin2 </it>roots. One of these proteins was identified as annexin2, which was induced in the root cap columella cells under altered gravitational conditions.</p> <p>Conclusions</p> <p>The most interesting observation in this study is that distinctly different patterns of protein expression were found in WT and <it>pin</it>2 mutant roots subjected to altered gravity conditions. The data also demonstrate that PIN2 mutation not only affects the basipetal transport of auxin to the elongation zone, but also results in an altered expression of proteins in the root columella.</p> |
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| spelling | doaj.art-0e1b60f808e24931a33c1cba61b240732022-12-22T01:41:49ZengBMCProteome Science1477-59562011-11-01917210.1186/1477-5956-9-72A proteomic approach to analyzing responses of <it>Arabidopsis thaliana </it>root cells to different gravitational conditions using an agravitropic mutant, <it>pin2 </it>and its wild typeTan ChaoWang HuiZhang YueQi BinXu GuoxinZheng Huiqiong<p>Abstract</p> <p>Background</p> <p>Root gravitropsim has been proposed to require the coordinated, redistribution of the plant signaling molecule auxin within the root meristem, but the underlying molecular mechanisms are still unknown. PIN proteins are membrane transporters that mediate the efflux of auxin from cells. The PIN2 is important for the basipetal transport of auxin in roots and plays a critical role in the transmission of gravity signals perceived in the root cap to the root elongation zone. The loss of function <it>pin2 </it>mutant exhibits a gravity-insensitive root growth phenotype. By comparing the proteomes of wild type and the <it>pin2 </it>mutant root tips under different gravitational conditions, we hope to identify proteins involved in the gravity-related signal transduction.</p> <p>Results</p> <p>To identify novel proteins involved in the gravity signal transduction pathway we have carried out a comparative proteomic analysis of Arabidopsis <it>pin2 </it>mutant and wild type (WT) roots subjected to different gravitational conditions. These conditions included horizontal (H) and vertical (V) clinorotation, hypergravity (G) and the stationary control (S). Analysis of silver-stained two-dimensional SDS-PAGE gels revealed 28 protein spots that showed significant expression changes in altered gravity (H or G) compared to control roots (V and S). Whereas the majority of these proteins exhibited similar expression patterns in WT and <it>pin2 </it>roots, a significant number displayed different patterns of response between WT and <it>pin2 </it>roots. The latter group included 11 protein spots in the H samples and two protein spots in the G samples that exhibited an altered expression exclusively in WT but not in <it>pin2 </it>roots. One of these proteins was identified as annexin2, which was induced in the root cap columella cells under altered gravitational conditions.</p> <p>Conclusions</p> <p>The most interesting observation in this study is that distinctly different patterns of protein expression were found in WT and <it>pin</it>2 mutant roots subjected to altered gravity conditions. The data also demonstrate that PIN2 mutation not only affects the basipetal transport of auxin to the elongation zone, but also results in an altered expression of proteins in the root columella.</p>http://www.proteomesci.com/content/9/1/72proteomicsAnnexinclinorotationhypergravity<it>Arabidopsis thaliana</it><it>pin2 mutant</it>root tip |
| spellingShingle | Tan Chao Wang Hui Zhang Yue Qi Bin Xu Guoxin Zheng Huiqiong A proteomic approach to analyzing responses of <it>Arabidopsis thaliana </it>root cells to different gravitational conditions using an agravitropic mutant, <it>pin2 </it>and its wild type Proteome Science proteomics Annexin clinorotation hypergravity <it>Arabidopsis thaliana</it> <it>pin2 mutant</it> root tip |
| title | A proteomic approach to analyzing responses of <it>Arabidopsis thaliana </it>root cells to different gravitational conditions using an agravitropic mutant, <it>pin2 </it>and its wild type |
| title_full | A proteomic approach to analyzing responses of <it>Arabidopsis thaliana </it>root cells to different gravitational conditions using an agravitropic mutant, <it>pin2 </it>and its wild type |
| title_fullStr | A proteomic approach to analyzing responses of <it>Arabidopsis thaliana </it>root cells to different gravitational conditions using an agravitropic mutant, <it>pin2 </it>and its wild type |
| title_full_unstemmed | A proteomic approach to analyzing responses of <it>Arabidopsis thaliana </it>root cells to different gravitational conditions using an agravitropic mutant, <it>pin2 </it>and its wild type |
| title_short | A proteomic approach to analyzing responses of <it>Arabidopsis thaliana </it>root cells to different gravitational conditions using an agravitropic mutant, <it>pin2 </it>and its wild type |
| title_sort | proteomic approach to analyzing responses of it arabidopsis thaliana it root cells to different gravitational conditions using an agravitropic mutant it pin2 it and its wild type |
| topic | proteomics Annexin clinorotation hypergravity <it>Arabidopsis thaliana</it> <it>pin2 mutant</it> root tip |
| url | http://www.proteomesci.com/content/9/1/72 |
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