Long-read sequencing and de novo assembly of a Chinese genome
Short-read sequencing has inherent limitations in the characterisation of long repeat elements. Shi and Guo et al.combine single-molecule real-time sequencing and IrysChip to construct a Chinese reference genome that fills many gaps in the reference genome, and identify novel spliced genes.
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2016-06-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/ncomms12065 |
| _version_ | 1818841438031446016 |
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| author | Lingling Shi Yunfei Guo Chengliang Dong John Huddleston Hui Yang Xiaolu Han Aisi Fu Quan Li Na Li Siyi Gong Katherine E. Lintner Qiong Ding Zou Wang Jiang Hu Depeng Wang Feng Wang Lin Wang Gholson J. Lyon Yongtao Guan Yufeng Shen Oleg V. Evgrafov James A. Knowles Francoise Thibaud-Nissen Valerie Schneider Chack-Yung Yu Libing Zhou Evan E. Eichler Kwok-Fai So Kai Wang |
| author_facet | Lingling Shi Yunfei Guo Chengliang Dong John Huddleston Hui Yang Xiaolu Han Aisi Fu Quan Li Na Li Siyi Gong Katherine E. Lintner Qiong Ding Zou Wang Jiang Hu Depeng Wang Feng Wang Lin Wang Gholson J. Lyon Yongtao Guan Yufeng Shen Oleg V. Evgrafov James A. Knowles Francoise Thibaud-Nissen Valerie Schneider Chack-Yung Yu Libing Zhou Evan E. Eichler Kwok-Fai So Kai Wang |
| author_sort | Lingling Shi |
| collection | DOAJ |
| description | Short-read sequencing has inherent limitations in the characterisation of long repeat elements. Shi and Guo et al.combine single-molecule real-time sequencing and IrysChip to construct a Chinese reference genome that fills many gaps in the reference genome, and identify novel spliced genes. |
| first_indexed | 2024-12-19T04:26:05Z |
| format | Article |
| id | doaj.art-0e208353827a44778269d1b511fa9076 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-19T04:26:05Z |
| publishDate | 2016-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-0e208353827a44778269d1b511fa90762022-12-21T20:36:00ZengNature PortfolioNature Communications2041-17232016-06-017111010.1038/ncomms12065Long-read sequencing and de novo assembly of a Chinese genomeLingling Shi0Yunfei Guo1Chengliang Dong2John Huddleston3Hui Yang4Xiaolu Han5Aisi Fu6Quan Li7Na Li8Siyi Gong9Katherine E. Lintner10Qiong Ding11Zou Wang12Jiang Hu13Depeng Wang14Feng Wang15Lin Wang16Gholson J. Lyon17Yongtao Guan18Yufeng Shen19Oleg V. Evgrafov20James A. Knowles21Francoise Thibaud-Nissen22Valerie Schneider23Chack-Yung Yu24Libing Zhou25Evan E. Eichler26Kwok-Fai So27Kai Wang28Guangdong-Hongkong-Macau Institute of CNS Regeneration, Jinan UniversityZilkha Neurogenetic Institute, University of Southern CaliforniaZilkha Neurogenetic Institute, University of Southern CaliforniaDepartment of Genome Sciences, Howard Hughes Medical Institute, University of WashingtonZilkha Neurogenetic Institute, University of Southern CaliforniaGenetic, Molecular, and Cellular Biology Program, Keck School of Medicine, University of Southern CaliforniaWuhan Institute of BiotechnologyZilkha Neurogenetic Institute, University of Southern CaliforniaGuangdong-Hongkong-Macau Institute of CNS Regeneration, Jinan UniversityGuangdong-Hongkong-Macau Institute of CNS Regeneration, Jinan UniversityDepartment of Pediatrics, The Ohio State University, and The Research Institute at Nationwide Children's HospitalWuhan Institute of BiotechnologyWuhan Institute of BiotechnologyNextomics BiosciencesNextomics BiosciencesSchool of Chemical Engineering and Pharmacy, Wuhan Institute of TechnologyCenter for Tissue Engineering and Regenerative Medicine, Union Hospital, Huazhong University of Science and TechnologyStanley Institute for Cognitive Genomics, Cold Spring Harbor LaboratoryDepartment of Pediatrics, Department of Molecular and Human Genetics, USDA/ARS Children's Nutrition Research Center, Baylor College of MedicineDepartments of Systems Biology and Biomedical Informatics, Columbia UniversityZilkha Neurogenetic Institute, University of Southern CaliforniaZilkha Neurogenetic Institute, University of Southern CaliforniaNational Center for Biotechnology Information, U.S. National Library of MedicineNational Center for Biotechnology Information, U.S. National Library of MedicineDepartment of Pediatrics, The Ohio State University, and The Research Institute at Nationwide Children's HospitalGuangdong-Hongkong-Macau Institute of CNS Regeneration, Jinan UniversityDepartment of Genome Sciences, Howard Hughes Medical Institute, University of WashingtonGuangdong-Hongkong-Macau Institute of CNS Regeneration, Jinan UniversityZilkha Neurogenetic Institute, University of Southern CaliforniaShort-read sequencing has inherent limitations in the characterisation of long repeat elements. Shi and Guo et al.combine single-molecule real-time sequencing and IrysChip to construct a Chinese reference genome that fills many gaps in the reference genome, and identify novel spliced genes.https://doi.org/10.1038/ncomms12065 |
| spellingShingle | Lingling Shi Yunfei Guo Chengliang Dong John Huddleston Hui Yang Xiaolu Han Aisi Fu Quan Li Na Li Siyi Gong Katherine E. Lintner Qiong Ding Zou Wang Jiang Hu Depeng Wang Feng Wang Lin Wang Gholson J. Lyon Yongtao Guan Yufeng Shen Oleg V. Evgrafov James A. Knowles Francoise Thibaud-Nissen Valerie Schneider Chack-Yung Yu Libing Zhou Evan E. Eichler Kwok-Fai So Kai Wang Long-read sequencing and de novo assembly of a Chinese genome Nature Communications |
| title | Long-read sequencing and de novo assembly of a Chinese genome |
| title_full | Long-read sequencing and de novo assembly of a Chinese genome |
| title_fullStr | Long-read sequencing and de novo assembly of a Chinese genome |
| title_full_unstemmed | Long-read sequencing and de novo assembly of a Chinese genome |
| title_short | Long-read sequencing and de novo assembly of a Chinese genome |
| title_sort | long read sequencing and de novo assembly of a chinese genome |
| url | https://doi.org/10.1038/ncomms12065 |
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