Epigenetic and non-epigenetic mode of SIRT1 action during oocyte meiosis progression
Abstract Background SIRT1 histone deacetylase acts on many epigenetic and non-epigenetic targets. It is thought that SIRT1 is involved in oocyte maturation; therefore, the importance of the ooplasmic SIRT1 pool for the further fate of mature oocytes has been strongly suggested. We hypothesised that...
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| Format: | Article |
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BMC
2019-08-01
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| Series: | Journal of Animal Science and Biotechnology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40104-019-0372-3 |
| _version_ | 1818316564137508864 |
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| author | Jan Nevoral Lukas Landsmann Miriam Stiavnicka Petr Hosek Jiri Moravec Sarka Prokesova Hedvika Rimnacova Eliska Koutna Pavel Klein Kristyna Hoskova Tereza Zalmanova Tereza Fenclova Jaroslav Petr Milena Kralickova |
| author_facet | Jan Nevoral Lukas Landsmann Miriam Stiavnicka Petr Hosek Jiri Moravec Sarka Prokesova Hedvika Rimnacova Eliska Koutna Pavel Klein Kristyna Hoskova Tereza Zalmanova Tereza Fenclova Jaroslav Petr Milena Kralickova |
| author_sort | Jan Nevoral |
| collection | DOAJ |
| description | Abstract Background SIRT1 histone deacetylase acts on many epigenetic and non-epigenetic targets. It is thought that SIRT1 is involved in oocyte maturation; therefore, the importance of the ooplasmic SIRT1 pool for the further fate of mature oocytes has been strongly suggested. We hypothesised that SIRT1 plays the role of a signalling molecule in mature oocytes through selected epigenetic and non-epigenetic regulation. Results We observed SIRT1 re-localisation in mature oocytes and its association with spindle microtubules. In mature oocytes, SIRT1 distribution shows a spindle-like pattern, and spindle-specific SIRT1 action decreases α-tubulin acetylation. Based on the observation of the histone code in immature and mature oocytes, we suggest that SIRT1 is mostly predestined for an epigenetic mode of action in the germinal vesicles (GVs) of immature oocytes. Accordingly, BML-278-driven trimethylation of lysine K9 in histone H3 in mature oocytes is considered to be a result of GV epigenetic transformation. Conclusions Taken together, our observations point out the dual spatiotemporal SIRT1 action in oocytes, which can be readily switched from the epigenetic to non-epigenetic mode of action depending on the progress of meiosis. |
| first_indexed | 2024-12-13T09:23:26Z |
| format | Article |
| id | doaj.art-0e230c035bf14471bca8b2c951c9c485 |
| institution | Directory Open Access Journal |
| issn | 2049-1891 |
| language | English |
| last_indexed | 2024-12-13T09:23:26Z |
| publishDate | 2019-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Animal Science and Biotechnology |
| spelling | doaj.art-0e230c035bf14471bca8b2c951c9c4852022-12-21T23:52:41ZengBMCJournal of Animal Science and Biotechnology2049-18912019-08-0110111210.1186/s40104-019-0372-3Epigenetic and non-epigenetic mode of SIRT1 action during oocyte meiosis progressionJan Nevoral0Lukas Landsmann1Miriam Stiavnicka2Petr Hosek3Jiri Moravec4Sarka Prokesova5Hedvika Rimnacova6Eliska Koutna7Pavel Klein8Kristyna Hoskova9Tereza Zalmanova10Tereza Fenclova11Jaroslav Petr12Milena Kralickova13Biomedical Center, Faculty of Medicine in Pilsen, Charles UniversityBiomedical Center, Faculty of Medicine in Pilsen, Charles UniversityBiomedical Center, Faculty of Medicine in Pilsen, Charles UniversityBiomedical Center, Faculty of Medicine in Pilsen, Charles UniversityBiomedical Center, Faculty of Medicine in Pilsen, Charles UniversityBiomedical Center, Faculty of Medicine in Pilsen, Charles UniversityBiomedical Center, Faculty of Medicine in Pilsen, Charles UniversityBiomedical Center, Faculty of Medicine in Pilsen, Charles UniversityBiomedical Center, Faculty of Medicine in Pilsen, Charles UniversityInstitute of Animal ScienceInstitute of Animal ScienceBiomedical Center, Faculty of Medicine in Pilsen, Charles UniversityInstitute of Animal ScienceBiomedical Center, Faculty of Medicine in Pilsen, Charles UniversityAbstract Background SIRT1 histone deacetylase acts on many epigenetic and non-epigenetic targets. It is thought that SIRT1 is involved in oocyte maturation; therefore, the importance of the ooplasmic SIRT1 pool for the further fate of mature oocytes has been strongly suggested. We hypothesised that SIRT1 plays the role of a signalling molecule in mature oocytes through selected epigenetic and non-epigenetic regulation. Results We observed SIRT1 re-localisation in mature oocytes and its association with spindle microtubules. In mature oocytes, SIRT1 distribution shows a spindle-like pattern, and spindle-specific SIRT1 action decreases α-tubulin acetylation. Based on the observation of the histone code in immature and mature oocytes, we suggest that SIRT1 is mostly predestined for an epigenetic mode of action in the germinal vesicles (GVs) of immature oocytes. Accordingly, BML-278-driven trimethylation of lysine K9 in histone H3 in mature oocytes is considered to be a result of GV epigenetic transformation. Conclusions Taken together, our observations point out the dual spatiotemporal SIRT1 action in oocytes, which can be readily switched from the epigenetic to non-epigenetic mode of action depending on the progress of meiosis.http://link.springer.com/article/10.1186/s40104-019-0372-3EpigeneticsHistone codeIn vitro maturationOocyteSIRT1Sirtuin 1 |
| spellingShingle | Jan Nevoral Lukas Landsmann Miriam Stiavnicka Petr Hosek Jiri Moravec Sarka Prokesova Hedvika Rimnacova Eliska Koutna Pavel Klein Kristyna Hoskova Tereza Zalmanova Tereza Fenclova Jaroslav Petr Milena Kralickova Epigenetic and non-epigenetic mode of SIRT1 action during oocyte meiosis progression Journal of Animal Science and Biotechnology Epigenetics Histone code In vitro maturation Oocyte SIRT1 Sirtuin 1 |
| title | Epigenetic and non-epigenetic mode of SIRT1 action during oocyte meiosis progression |
| title_full | Epigenetic and non-epigenetic mode of SIRT1 action during oocyte meiosis progression |
| title_fullStr | Epigenetic and non-epigenetic mode of SIRT1 action during oocyte meiosis progression |
| title_full_unstemmed | Epigenetic and non-epigenetic mode of SIRT1 action during oocyte meiosis progression |
| title_short | Epigenetic and non-epigenetic mode of SIRT1 action during oocyte meiosis progression |
| title_sort | epigenetic and non epigenetic mode of sirt1 action during oocyte meiosis progression |
| topic | Epigenetics Histone code In vitro maturation Oocyte SIRT1 Sirtuin 1 |
| url | http://link.springer.com/article/10.1186/s40104-019-0372-3 |
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