E-test versus agar dilution for antibiotic susceptibility testing of Helicobacter pylori: a comparison study
Abstract Objective For evaluating the antibiotic resistance of Helicobacter pylori, the agar dilution method is the gold standard; however, using this method in daily practice is laborious. E-test has been proposed to be an uncomplicated method. This study was aimed at validating the E-test and dete...
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| Format: | Article |
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BMC
2020-01-01
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| Series: | BMC Research Notes |
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| Online Access: | https://doi.org/10.1186/s13104-019-4877-9 |
| _version_ | 1818453316822106112 |
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| author | Muhammad Miftahussurur Kartika Afrida Fauzia Iswan Abbas Nusi Poernomo Boedi Setiawan Ari Fahrial Syam Langgeng Agung Waskito Dalla Doohan Neneng Ratnasari Ali Khomsan I. Ketut Adnyana Junko Akada Yoshio Yamaoka |
| author_facet | Muhammad Miftahussurur Kartika Afrida Fauzia Iswan Abbas Nusi Poernomo Boedi Setiawan Ari Fahrial Syam Langgeng Agung Waskito Dalla Doohan Neneng Ratnasari Ali Khomsan I. Ketut Adnyana Junko Akada Yoshio Yamaoka |
| author_sort | Muhammad Miftahussurur |
| collection | DOAJ |
| description | Abstract Objective For evaluating the antibiotic resistance of Helicobacter pylori, the agar dilution method is the gold standard; however, using this method in daily practice is laborious. E-test has been proposed to be an uncomplicated method. This study was aimed at validating the E-test and detecting the presence of any bias between the agar dilution method and E-test. Results The agar dilution method and E-test were performed using five antibiotics for 72 strains of H. pylori obtained from clinical patients in Indonesia. The E-test’s results showed a higher prevalence of resistance to all the antibiotics tested but the difference was not significant. Results showed high essential agreement (> 90.0%) for all the antibiotics, but only 84.7% for metronidazole. The agreement for MIC value was acceptable for levofloxacin, clarithromycin, and metronidazole. For amoxicillin, it showed only fair agreement (0.25) by the Kappa analysis and significant difference by Passing-Bablok regression. Even though some discrepancies were found, the E-test has an acceptable agreement for levofloxacin, metronidazole, tetracycline, and clarithromycin but further confirmation may be necessary for amoxicillin. |
| first_indexed | 2024-12-14T21:37:03Z |
| format | Article |
| id | doaj.art-0e28a635440546d09837d38abb89a67b |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-14T21:37:03Z |
| publishDate | 2020-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-0e28a635440546d09837d38abb89a67b2022-12-21T22:46:33ZengBMCBMC Research Notes1756-05002020-01-011311610.1186/s13104-019-4877-9E-test versus agar dilution for antibiotic susceptibility testing of Helicobacter pylori: a comparison studyMuhammad Miftahussurur0Kartika Afrida Fauzia1Iswan Abbas Nusi2Poernomo Boedi Setiawan3Ari Fahrial Syam4Langgeng Agung Waskito5Dalla Doohan6Neneng Ratnasari7Ali Khomsan8I. Ketut Adnyana9Junko Akada10Yoshio Yamaoka11Division of Gastroentero-Hepatology, Department of Internal Medicine, Faculty of Medicine-Dr. Soetomo Teaching Hospital, Universitas AirlanggaInstitute of Tropical Disease, Universitas AirlanggaDivision of Gastroentero-Hepatology, Department of Internal Medicine, Faculty of Medicine-Dr. Soetomo Teaching Hospital, Universitas AirlanggaDivision of Gastroentero-Hepatology, Department of Internal Medicine, Faculty of Medicine-Dr. Soetomo Teaching Hospital, Universitas AirlanggaDivision of Gastroenterology, Department of Internal Medicine, Faculty of Medicine, University of IndonesiaInstitute of Tropical Disease, Universitas AirlanggaInstitute of Tropical Disease, Universitas AirlanggaDepartment of Internal Medicine, Faculty of Medicine Gadjah, Mada University-Dr. SardjitoDepartment of Community Nutrition, Bogor Agriculture UniversitySchool of Pharmacy, Bandung Institute of TechnologyDepartment of Environmental and Preventive Medicine, Oita University Faculty of MedicineDivision of Gastroentero-Hepatology, Department of Internal Medicine, Faculty of Medicine-Dr. Soetomo Teaching Hospital, Universitas AirlanggaAbstract Objective For evaluating the antibiotic resistance of Helicobacter pylori, the agar dilution method is the gold standard; however, using this method in daily practice is laborious. E-test has been proposed to be an uncomplicated method. This study was aimed at validating the E-test and detecting the presence of any bias between the agar dilution method and E-test. Results The agar dilution method and E-test were performed using five antibiotics for 72 strains of H. pylori obtained from clinical patients in Indonesia. The E-test’s results showed a higher prevalence of resistance to all the antibiotics tested but the difference was not significant. Results showed high essential agreement (> 90.0%) for all the antibiotics, but only 84.7% for metronidazole. The agreement for MIC value was acceptable for levofloxacin, clarithromycin, and metronidazole. For amoxicillin, it showed only fair agreement (0.25) by the Kappa analysis and significant difference by Passing-Bablok regression. Even though some discrepancies were found, the E-test has an acceptable agreement for levofloxacin, metronidazole, tetracycline, and clarithromycin but further confirmation may be necessary for amoxicillin.https://doi.org/10.1186/s13104-019-4877-9E-testAgar dilutionHelicobacter pyloriAntibiotic susceptibility test |
| spellingShingle | Muhammad Miftahussurur Kartika Afrida Fauzia Iswan Abbas Nusi Poernomo Boedi Setiawan Ari Fahrial Syam Langgeng Agung Waskito Dalla Doohan Neneng Ratnasari Ali Khomsan I. Ketut Adnyana Junko Akada Yoshio Yamaoka E-test versus agar dilution for antibiotic susceptibility testing of Helicobacter pylori: a comparison study BMC Research Notes E-test Agar dilution Helicobacter pylori Antibiotic susceptibility test |
| title | E-test versus agar dilution for antibiotic susceptibility testing of Helicobacter pylori: a comparison study |
| title_full | E-test versus agar dilution for antibiotic susceptibility testing of Helicobacter pylori: a comparison study |
| title_fullStr | E-test versus agar dilution for antibiotic susceptibility testing of Helicobacter pylori: a comparison study |
| title_full_unstemmed | E-test versus agar dilution for antibiotic susceptibility testing of Helicobacter pylori: a comparison study |
| title_short | E-test versus agar dilution for antibiotic susceptibility testing of Helicobacter pylori: a comparison study |
| title_sort | e test versus agar dilution for antibiotic susceptibility testing of helicobacter pylori a comparison study |
| topic | E-test Agar dilution Helicobacter pylori Antibiotic susceptibility test |
| url | https://doi.org/10.1186/s13104-019-4877-9 |
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