Improved fertility following a gonadotropin-releasing hormone treatment on day 2 of an estradiol and progesterone-based timed-artificial insemination protocol in lactating dairy cows
The present study evaluated the addition of gonadotropin-releasing hormone (GnRH) concomitant or 2 d after the beginning of protocols initiated with estradiol benzoate (EB). A total of 459 multiparous and 371 primiparous lactating Holstein cows were enrolled in the study. Weekly cohorts of cows were...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-05-01
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| Series: | JDS Communications |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666910222000497 |
| _version_ | 1797788366138245120 |
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| author | Carlos E.C. Consentini Tiago O. Carneiro Humberto Neri Emiliana O.S. Batista Lucas O. e Silva Alexandre H. Souza Roberto Sartori |
| author_facet | Carlos E.C. Consentini Tiago O. Carneiro Humberto Neri Emiliana O.S. Batista Lucas O. e Silva Alexandre H. Souza Roberto Sartori |
| author_sort | Carlos E.C. Consentini |
| collection | DOAJ |
| description | The present study evaluated the addition of gonadotropin-releasing hormone (GnRH) concomitant or 2 d after the beginning of protocols initiated with estradiol benzoate (EB). A total of 459 multiparous and 371 primiparous lactating Holstein cows were enrolled in the study. Weekly cohorts of cows were randomly assigned to 1 of 3 experimental groups that differed in the strategy to initiate the timed AI (TAI protocol. On d 0, all cows received a 1.55-g progesterone (P4) implant. Additionally, cows in the EBd0 group received 2 mg of EB i.m.; cows in the EBd0-GnRHd0 group were treated simultaneously on d 0 with 2 mg of EB plus 100 µg of gonadorelin diacetate tetrahydrate (GnRH) i.m.; and cows in the EBd0-GnRHd2 group received 2 mg of EB on d 0 and 100 µg of GnRH 48 h later (d 2). The remaining treatments in the protocol were similar among groups and included 0.53 mg (i.m.) of cloprostenol sodium (PGF2α) on d 7, followed by a second PGF2α treatment on d 9 (at the time of P4 implant withdrawal) and 1 mg of estradiol cypionate i.m. Then, TAI was performed on d 11 (48 h after P4 removal) in all experimental groups. We detected an effect of treatment on pregnancy per AI (P/AI) on d 30, in which cows from the EBd0-GnRHd2 group demonstrated greater fertility than EBd0 cows, whereas cows in the EBd0-GnRHd0 group did not differ among EBd0 and EBd0-GnRHd0 (40.5 vs. 30.4 vs. 34.4%, respectively). In summary, GnRH treatment at the beginning of an estradiol and P4-based TAI protocol increased fertility only when GnRH was given on d 2. Moreover, a more pronounced positive effect of this strategy was observed in particular classes of cows: multiparous cows, cows with greater milk production, and those receiving the first service. |
| first_indexed | 2024-03-13T01:35:33Z |
| format | Article |
| id | doaj.art-0e28ff4033764a709fd474be1364fd11 |
| institution | Directory Open Access Journal |
| issn | 2666-9102 |
| language | English |
| last_indexed | 2024-03-13T01:35:33Z |
| publishDate | 2022-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | JDS Communications |
| spelling | doaj.art-0e28ff4033764a709fd474be1364fd112023-07-04T05:10:55ZengElsevierJDS Communications2666-91022022-05-0133212216Improved fertility following a gonadotropin-releasing hormone treatment on day 2 of an estradiol and progesterone-based timed-artificial insemination protocol in lactating dairy cowsCarlos E.C. Consentini0Tiago O. Carneiro1Humberto Neri2Emiliana O.S. Batista3Lucas O. e Silva4Alexandre H. Souza5Roberto Sartori6Department of Animal Science, University of São Paulo, Piracicaba, SP, Brazil, 13418-900Bela Vista Farm, Tapiratiba, SP, Brazil, 13760-000Biotran Biotecnologia, Alfenas, MG, Brazil, 37132-346Adventist University Center of São Paulo, Engenheiro Coelho, São Paulo, Brazil, 13165-970Department of Animal Science, University of São Paulo, Piracicaba, SP, Brazil, 13418-900Cargill Animal Nutrition and Health, Campinas, SP, Brazil, 13091-611Department of Animal Science, University of São Paulo, Piracicaba, SP, Brazil, 13418-900; Corresponding author:The present study evaluated the addition of gonadotropin-releasing hormone (GnRH) concomitant or 2 d after the beginning of protocols initiated with estradiol benzoate (EB). A total of 459 multiparous and 371 primiparous lactating Holstein cows were enrolled in the study. Weekly cohorts of cows were randomly assigned to 1 of 3 experimental groups that differed in the strategy to initiate the timed AI (TAI protocol. On d 0, all cows received a 1.55-g progesterone (P4) implant. Additionally, cows in the EBd0 group received 2 mg of EB i.m.; cows in the EBd0-GnRHd0 group were treated simultaneously on d 0 with 2 mg of EB plus 100 µg of gonadorelin diacetate tetrahydrate (GnRH) i.m.; and cows in the EBd0-GnRHd2 group received 2 mg of EB on d 0 and 100 µg of GnRH 48 h later (d 2). The remaining treatments in the protocol were similar among groups and included 0.53 mg (i.m.) of cloprostenol sodium (PGF2α) on d 7, followed by a second PGF2α treatment on d 9 (at the time of P4 implant withdrawal) and 1 mg of estradiol cypionate i.m. Then, TAI was performed on d 11 (48 h after P4 removal) in all experimental groups. We detected an effect of treatment on pregnancy per AI (P/AI) on d 30, in which cows from the EBd0-GnRHd2 group demonstrated greater fertility than EBd0 cows, whereas cows in the EBd0-GnRHd0 group did not differ among EBd0 and EBd0-GnRHd0 (40.5 vs. 30.4 vs. 34.4%, respectively). In summary, GnRH treatment at the beginning of an estradiol and P4-based TAI protocol increased fertility only when GnRH was given on d 2. Moreover, a more pronounced positive effect of this strategy was observed in particular classes of cows: multiparous cows, cows with greater milk production, and those receiving the first service.http://www.sciencedirect.com/science/article/pii/S2666910222000497 |
| spellingShingle | Carlos E.C. Consentini Tiago O. Carneiro Humberto Neri Emiliana O.S. Batista Lucas O. e Silva Alexandre H. Souza Roberto Sartori Improved fertility following a gonadotropin-releasing hormone treatment on day 2 of an estradiol and progesterone-based timed-artificial insemination protocol in lactating dairy cows JDS Communications |
| title | Improved fertility following a gonadotropin-releasing hormone treatment on day 2 of an estradiol and progesterone-based timed-artificial insemination protocol in lactating dairy cows |
| title_full | Improved fertility following a gonadotropin-releasing hormone treatment on day 2 of an estradiol and progesterone-based timed-artificial insemination protocol in lactating dairy cows |
| title_fullStr | Improved fertility following a gonadotropin-releasing hormone treatment on day 2 of an estradiol and progesterone-based timed-artificial insemination protocol in lactating dairy cows |
| title_full_unstemmed | Improved fertility following a gonadotropin-releasing hormone treatment on day 2 of an estradiol and progesterone-based timed-artificial insemination protocol in lactating dairy cows |
| title_short | Improved fertility following a gonadotropin-releasing hormone treatment on day 2 of an estradiol and progesterone-based timed-artificial insemination protocol in lactating dairy cows |
| title_sort | improved fertility following a gonadotropin releasing hormone treatment on day 2 of an estradiol and progesterone based timed artificial insemination protocol in lactating dairy cows |
| url | http://www.sciencedirect.com/science/article/pii/S2666910222000497 |
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